Restriction map for pF1KB5640
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AviII       AvrII       BamHI       
BbrPI       BbvCI       BcgI        BfrI        BglII       
BlnI        BsaAI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MfeI        MflI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfcI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          976  GTCTAC               GT^[AC][GT]AC
AcyI          263  GGCGCC               G[GA]^CG[CT]C
AflIII        151  ACATGT               A^C[GA][CT]GT
AlwI         1391  GGATC                GGATC(4/5)
ApaI          709  GGGCCC               GGGCC^C
AspI          478  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          167  CTCGGG               C^[CT]CG[GA]G
BalI            2  TGGCCA               TGG^CCA
BbeI          263  GGCGCC               GGCGC^C
BciVI         360  GGATAC               GTATCC(6/5)
BfaI         1465  CTAG                 C^TAG
BglI          334  GCCACCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1148  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1148  GCTCAGC              GC^T[ACGT]AGC
BsaBI          99  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         263  GGCGCC               G[GA]^CG[CT]C
BsaI         1031  GAGACC               GGTCTC(1/5)
BsmBI         352  GAGACG               CGTCTC(1/5)
BsmI         1138  GAATGC               GAATGC(1/-1)
BsoBI         167  CTCGGG               C^[CT]CG[GA]G
BspLU11I      151  ACATGT               A^CATGT
BspMI         972  GCAGGT               ACCTGC(4/8)
BstAPI        232  GCACAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1148  GCTCAGC              GC^T[ACGT]AGC
DraI         1438  TTTAAA               TTT^AAA
DraII        1059  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EarI         1171  CTCTTC               CTCTTC(1/4)
EcoNI         242  CCTTCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1059  GGGGCCT              [GA]G^G[ACGT]CC[CT]
HaeII         263  GGCGCC               [GA]GCGC^[CT]
Hin1I         263  GGCGCC               G[GA]^CG[CT]C
KasI          263  GGCGCC               G^GCGCC
Ksp632I      1171  CTCTTC               CTCTTC(1/4)
MaeI         1465  CTAG                 C^TAG
MamI           99  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MslI         1491  CACAGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        304  CAGCTG               C[AC]G^C[GT]G
NarI          263  GGCGCC               GG^CGCC
PflFI         478  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1426  CCATTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         304  CAGCTG               CAG^CTG
SapI         1170  GCTCTTC              GCTCTTC(1/4)
SexAI         314  ACCTGGT              A^CC[AT]GGT
SfoI          263  GGCGCC               GGC^GCC
Tsp509I       729  AATT                 ^AATT
Tth111I       478  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1426  CCATTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII          62  CGCG                 CG^CG
              331  CGCG                
AvaII         327  GGACC                G^G[AT]CC
              603  GGTCC               
BanI          263  GGCGCC               G^G[CT][GA]CC
              573  GGCACC              
BbsI         1159  GAAGAC               GAAGAC(2/6)
             1189  GTCTTC              
BclI          656  TGATCA               T^GATCA
             1071  TGATCA              
BcnI          668  CCGGG                CC^[GC]GG
              809  CCCGG               
BpuAI        1159  GAAGAC               GAAGAC(2/6)
             1189  GTCTTC              
BseRI         967  GAGGAG               GAGGAG(10/8)
             1228  GAGGAG              
BsgI          230  CTGCAC               GTGCAG(16/14)
              298  CTGCAC              
BsmAI         352  GAGAC                GTCTC(1/5)
             1031  GAGAC               
BsmFI         326  GGGAC                GGGAC(10/14)
              807  GTCCC               
BstUI          62  CGCG                 CG^CG
              331  CGCG                
FbaI          656  TGATCA               T^GATCA
             1071  TGATCA              
MaeII         209  ACGT                 A^CGT
              482  ACGT                
MseI          794  TTAA                 T^TAA
             1439  TTAA                
MvnI           62  CGCG                 CG^CG
              331  CGCG                
NaeI           51  GCCGGC               GCC^GGC
              266  GCCGGC              
NciI          668  CCGGG                CC^[GC]GG
              809  CCCGG               
NgoMIV         51  GCCGGC               G^CCGGC
              266  GCCGGC              
NspI          151  ACATGT               [GA]CATG^[CT]
             1211  ACATGC              
TfiI          124  GAATC                G^A[AT]TC
              704  GATTC               
Tru9I         794  TTAA                 T^TAA
             1439  TTAA                
XcmI           68  CCATCCGCAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              953  CCAGGGTGTTCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BpmI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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