Restriction map for pF1KB5640
 (Linear) (Six-base) MAPSORT of: KIBB5640.seq  Check: 4254  from: 1  to: 1536



KIBB5640.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0     977    1536 
   Size:        977     559
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     841    1279    1375    1536 
   Size:        628     213     438      96     161

  Fragments arranged by size: 

                 628     438     213     161      96
 
AflIII A'CryG_T

Cuts at:      0     151    1536 
   Size:        151    1385
 
AleI CACnn'nnGTG

Cuts at:      0    1495    1536 
   Size:       1495      41
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      73     105    1536 
   Size:         73      32    1431

  Fragments arranged by size: 

                1431      73      32
 
ApaI G_GGCC'C

Cuts at:      0     713    1536 
   Size:        713     823
 
AvaI C'yCGr_G

Cuts at:      0     167    1536 
   Size:        167    1369
 
BanI G'GyrC_C

Cuts at:      0     263     573    1536 
   Size:        263     310     963

  Fragments arranged by size: 

                 963     310     263
 
BanII G_rGCy'C

Cuts at:      0     256     509     713    1150    1172    1536 
   Size:        256     253     204     437      22     364

  Fragments arranged by size: 

                 437     364     256     253     204      22
 
BbeI G_GCGC'C

Cuts at:      0     267    1536 
   Size:        267    1269
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1166    1182    1536 
   Size:       1166      16     354

  Fragments arranged by size: 

                1166     354      16
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     354    1536 
   Size:        354    1182
 
BclI T'GATC_A

Cuts at:      0     656    1071    1536 
   Size:        656     415     465

  Fragments arranged by size: 

                 656     465     415
 
BglI GCCn_nnn'nGGC

Cuts at:      0     340    1536 
   Size:        340    1196
 
BlpI GC'TnA_GC

Cuts at:      0    1149    1536 
   Size:       1149     387
 
Bme1580I G_kGCm'C

Cuts at:      0     713     906    1536 
   Size:        713     193     630

  Fragments arranged by size: 

                 713     630     193
 
BmgBI CAC'GTC

Cuts at:      0     210    1536 
   Size:        210    1326
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     370     421     662     856     936    1536 
   Size:        100     270      51     241     194      80     600

  Fragments arranged by size: 

                 600     270     241     194     100      80      51
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1025    1536 
   Size:       1025     511
 
BsaBI GATnn'nnATC

Cuts at:      0     103    1536 
   Size:        103    1433
 
BsaHI Gr'CG_yC

Cuts at:      0     264    1536 
   Size:        264    1272
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      73     103    1536 
   Size:         73      30    1433

  Fragments arranged by size: 

                1433      73      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     982    1243    1536 
   Size:        982     261     293

  Fragments arranged by size: 

                 982     293     261
 
BseYI C'CCAG_C

Cuts at:      0     840    1536 
   Size:        840     696
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     283    1536 
   Size:        215      68    1253

  Fragments arranged by size: 

                1253     215      68
 
BsmI GAATG_Cn'

Cuts at:      0    1144    1536 
   Size:       1144     392
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     346    1536 
   Size:        346    1190
 
Bsp1286I G_dGCh'C

Cuts at:      0     256     509     713     906    1150    1172    1536 
   Size:        256     253     204     193     244      22     364

  Fragments arranged by size: 

                 364     256     253     244     204     193      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     963    1536 
   Size:        963     573
 
BsrFI r'CCGG_y

Cuts at:      0      51     266     766    1536 
   Size:         51     215     500     770

  Fragments arranged by size: 

                 770     500     215      51
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     238    1536 
   Size:        238    1298
 
BtgI C'CryG_G

Cuts at:      0      20     259     535     599    1025    1154    1536 
   Size:         20     239     276      64     426     129     382

  Fragments arranged by size: 

                 426     382     276     239     129      64      20
 
BtsI GCAGTG_nn'

Cuts at:      0     899    1536 
   Size:        899     637
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     762     797    1536 
   Size:        762      35     739

  Fragments arranged by size: 

                 762     739      35
 
DraI TTT'AAA

Cuts at:      0    1440    1536 
   Size:       1440      96
 
EaeI y'GGCC_r

Cuts at:      0       2     341    1481    1536 
   Size:          2     339    1140      55

  Fragments arranged by size: 

                1140     339      55       2
 
EarI CTCTTCn'nnn_

Cuts at:      0    1177    1536 
   Size:       1177     359
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1011    1536 
   Size:       1011     525
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     370     421     628     662     841     856     936 
   Size:        100     270      51     207      34     179      15      80

Cuts at:    936    1279    1375    1536 
   Size:        343      96     161

  Fragments arranged by size: 

                 343     270     207     179     161     100      96      80
                  51      34      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     246    1536 
   Size:        246    1290
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1060    1536 
   Size:       1060     476
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     777    1188    1536 
   Size:        777     411     348

  Fragments arranged by size: 

                 777     411     348
 
HaeII r_GCGC'y

Cuts at:      0     267    1536 
   Size:        267    1269
 
KasI G'GCGC_C

Cuts at:      0     263    1536 
   Size:        263    1273
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     762    1536 
   Size:        762     774
 
MscI TGG'CCA

Cuts at:      0       4    1536 
   Size:          4    1532
 
MslI CAynn'nnrTG

Cuts at:      0    1495    1536 
   Size:       1495      41
 
MspA1I CmG'CkG

Cuts at:      0     306    1536 
   Size:        306    1230
 
NaeI GCC'GGC

Cuts at:      0      53     268    1536 
   Size:         53     215    1268

  Fragments arranged by size: 

                1268     215      53
 
NarI GG'CG_CC

Cuts at:      0     264    1536 
   Size:        264    1272
 
NcoI C'CATG_G

Cuts at:      0     259     535     599    1154    1536 
   Size:        259     276      64     555     382

  Fragments arranged by size: 

                 555     382     276     259      64
 
NgoMIV G'CCGG_C

Cuts at:      0      51     266    1536 
   Size:         51     215    1270

  Fragments arranged by size: 

                1270     215      51
 
NspI r_CATG'y

Cuts at:      0     155    1215    1536 
   Size:        155    1060     321

  Fragments arranged by size: 

                1060     321     155
 
PciI A'CATG_T

Cuts at:      0     151    1536 
   Size:        151    1385
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1432    1536 
   Size:       1432     104
 
PfoI T'CCnGG_A

Cuts at:      0     678     808    1536 
   Size:        678     130     728

  Fragments arranged by size: 

                 728     678     130
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      73     105     655     687    1536 
   Size:         73      32     550      32     849

  Fragments arranged by size: 

                 849     550      73      32      32
 
PspOMI G'GGCC_C

Cuts at:      0     709    1536 
   Size:        709     827
 
PvuII CAG'CTG

Cuts at:      0     306    1536 
   Size:        306    1230
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1177    1536 
   Size:       1177     359
 
SexAI A'CCwGG_T

Cuts at:      0     314    1536 
   Size:        314    1222
 
SfoI GGC'GCC

Cuts at:      0     265    1536 
   Size:        265    1271
 
StyI C'CwwG_G

Cuts at:      0     259     338     535     599     927    1154    1536 
   Size:        259      79     197      64     328     227     382

  Fragments arranged by size: 

                 382     328     259     227     197      79      64
 
Tth111I GACn'n_nGTC

Cuts at:      0     481    1536 
   Size:        481    1055
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      75     960    1536 
   Size:         75     885     576

  Fragments arranged by size: 

                 885     576      75
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AloI     ApaI     AvaI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BpmI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI     BsmI    BsmBI Bsp1286I    BspMI    BsrFI   BstAPI     BtgI
     BtsI    CspCI     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI
     PpiI   PspOMI    PvuII     SapI    SexAI     SfoI     StyI  Tth111I
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BfrBI    BglII     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaWI    BsiEI  BsiHKAI    BsiWI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII
     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI
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