Restriction map for pF1KB5634
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BamHI       
BbrPI       BcgI        BfrI        BlnI        BsaAI       
BsaBI       BsiEI       BsiWI       Bsp1407I    BspLU11I    
BsrBI       BsrDI       BsrGI       Bst1107I    BstBI       
BstZ17I     Bsu36I      CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FseI        HincII      HindII      HpaI        KpnI        
MamI        MfeI        MluI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1309  GTCTAC               GT^[AC][GT]AC
AccIII       1871  TCCGGA               T^CCGGA
AcsI         1543  AAATTC               [GA]^AATT[CT]
AflIII        302  ACGTGT               A^C[GA][CT]GT
AlwI         1911  GATCC                GGATC(4/5)
ApaI         2101  GGGCCC               GGGCC^C
ApoI         1543  AAATTC               [GA]^AATT[CT]
AspHI        1984  GTGCTC               G[AT]GC[AT]^C
AviII        2055  TGCGCA               TGC^GCA
BbvCI        2226  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1216  GGATAC               GTATCC(6/5)
BclI         1838  TGATCA               T^GATCA
BfaI         1202  CTAG                 C^TAG
BglII        1496  AGATCT               A^GATCT
BlpI         2311  GCTGAGC              GC^T[ACGT]AGC
BpmI          391  CTGGAG               CTGGAG(16/14)
Bpu1102I     2311  GCTGAGC              GC^T[ACGT]AGC
BsaI          551  GAGACC               GGTCTC(1/5)
BseAI        1871  TCCGGA               T^CCGGA
BsiHKAI      1984  GTGCTC               G[AT]GC[AT]^C
BsmBI         299  GAGACG               CGTCTC(1/5)
BsmI          234  GAATGC               GAATGC(1/-1)
BspDI         604  ATCGAT               AT^CGAT
BspEI        1871  TCCGGA               T^CCGGA
BsrFI         125  ACCGGC               [GA]^CCGG[CT]
BssHII       1886  GCGCGC               G^CGCGC
BssSI        1915  CACGAG               CACGAG(-5/-1)
BstEII       1926  GGTCACC              G^GT[ACGT]ACC
BstPI        1926  GGTCACC              G^GT[ACGT]ACC
CelII        2311  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        125  ACCGGC               [GA]^CCGG[CT]
ClaI          604  ATCGAT               AT^CGAT
DrdI         1300  GACAGAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          172  GAAGAG               CTCTTC(1/4)
EcoO65I      1926  GGTCACC              G^GT[ACGT]ACC
FbaI         1838  TGATCA               T^GATCA
FspI         2055  TGCGCA               TGC^GCA
HindIII       639  AAGCTT               A^AGCTT
Ksp632I       172  GAAGAG               CTCTTC(1/4)
MaeI         1202  CTAG                 C^TAG
MroI         1871  TCCGGA               T^CCGGA
PflMI        1794  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         501  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1492  CTGCAG               CTGCA^G
SfcI         1492  CTGCAG               C^T[GA][CT]AG
SgrAI         124  CACCGGCG             C[GA]^CCGG[CT]G
Van91I       1794  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

BalI         1181  TGGCCA               TGG^CCA
             1479  TGGCCA              
BbeI           34  GGCGCC               GGCGC^C
              128  GGCGCC              
BbsI          805  GAAGAC               GAAGAC(2/6)
              274  GTCTTC              
BglI         2016  GCCCCCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2070  GCCACCGAGGC         
BpuAI         805  GAAGAC               GAAGAC(2/6)
              274  GTCTTC              
BsaWI         869  ACCGGA               [AT]^CCGG[AT]
             1871  TCCGGA              
BsgI         2182  CTGCAC               GTGCAG(16/14)
             2062  GTGCAG              
BspHI          11  TCATGA               T^CATGA
              423  TCATGA              
BstAPI         73  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              523  GCAGTGGATGC         
BstXI         725  CCACCGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1340  CCAAAGAACTGG        
BstYI        1496  AGATCT               [GA]^GATC[CT]
             1910  AGATCC              
HaeII          34  GGCGCC               [GA]GCGC^[CT]
              128  GGCGCC              
KasI           34  GGCGCC               G^GCGCC
              128  GGCGCC              
MflI         1496  AGATCT               [GA]^GATC[CT]
             1910  AGATCC              
MluNI        1181  TGGCCA               TGG^CCA
             1479  TGGCCA              
MscI         1181  TGGCCA               TGG^CCA
             1479  TGGCCA              
MseI         2279  TTAA                 T^TAA
             2300  TTAA                
NarI           34  GGCGCC               GG^CGCC
              128  GGCGCC              
NcoI         1184  CCATGG               C^CATGG
             1476  CCATGG              
RcaI           11  TCATGA               T^CATGA
              423  TCATGA              
SfoI           34  GGCGCC               GGC^GCC
              128  GGCGCC              
SmaI         2104  CCCGGG               CCC^GGG
             2333  CCCGGG              
TfiI         1351  GATTC                G^A[AT]TC
             1378  GAATC               
Tru9I        2279  TTAA                 T^TAA
             2300  TTAA                
Tsp509I       610  AATT                 ^AATT
             1544  AATT                
XcmI          311  CCATTGAGAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              962  CCATGAAGCCTCTGG     
XhoII        1496  AGATCT               [GA]^GATC[CT]
             1910  AGATCC              
XmaCI        2104  CCCGGG               C^CCGGG
             2333  CCCGGG              
XmaI         2104  CCCGGG               C^CCGGG
             2333  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BmyI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KspI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PvuII       
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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