Restriction map for pF1KB5633
 (Linear) (Six-base) MAPSORT of: KIBB5633.seq  Check: 2452  from: 1  to: 1566



KIBB5633.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556    1168    1334    1566 
   Size:        556     612     166     232

  Fragments arranged by size: 

                 612     556     232     166
 
AfeI AGC'GCT

Cuts at:      0      84    1204    1566 
   Size:         84    1120     362

  Fragments arranged by size: 

                1120     362      84
 
AleI CACnn'nnGTG

Cuts at:      0    1041    1566 
   Size:       1041     525
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1059    1192    1566 
   Size:       1059     133     374

  Fragments arranged by size: 

                1059     374     133
 
AlwNI CAG_nnn'CTG

Cuts at:      0      77    1566 
   Size:         77    1489
 
ApoI r'AATT_y

Cuts at:      0     511     879    1052    1566 
   Size:        511     368     173     514

  Fragments arranged by size: 

                 514     511     368     173
 
AseI AT'TA_AT

Cuts at:      0     521    1566 
   Size:        521    1045
 
AvaI C'yCGr_G

Cuts at:      0    1153    1566 
   Size:       1153     413
 
BamHI G'GATC_C

Cuts at:      0      31    1566 
   Size:         31    1535
 
BanII G_rGCy'C

Cuts at:      0     989    1566 
   Size:        989     577
 
BclI T'GATC_A

Cuts at:      0    1305    1566 
   Size:       1305     261
 
BfrBI ATG'CAT

Cuts at:      0    1086    1431    1566 
   Size:       1086     345     135

  Fragments arranged by size: 

                1086     345     135
 
BlpI GC'TnA_GC

Cuts at:      0    1199    1566 
   Size:       1199     367
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     792    1566 
   Size:        792     774
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120    1566 
   Size:        120    1446
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463    1030    1566 
   Size:        463     567     536

  Fragments arranged by size: 

                 567     536     463
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1500    1566 
   Size:       1500      66
 
BsaWI w'CCGG_w

Cuts at:      0     875    1566 
   Size:        875     691
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1518    1548    1566 
   Size:       1518      30      18

  Fragments arranged by size: 

                1518      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     166    1120    1566 
   Size:        166     954     446

  Fragments arranged by size: 

                 954     446     166
 
BsiHKAI G_wGCw'C

Cuts at:      0     989    1566 
   Size:        989     577
 
BsmI GAATG_Cn'

Cuts at:      0     211    1088    1566 
   Size:        211     877     478

  Fragments arranged by size: 

                 877     478     211
 
Bsp1286I G_dGCh'C

Cuts at:      0     989    1566 
   Size:        989     577
 
BspEI T'CCGG_A

Cuts at:      0     875    1566 
   Size:        875     691
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     292    1566 
   Size:        292    1274
 
BsrDI GCAATG_nn'

Cuts at:      0    1053    1566 
   Size:       1053     513
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     275    1566 
   Size:        275    1291
 
BstBI TT'CG_AA

Cuts at:      0    1002    1566 
   Size:       1002     564
 
BstYI r'GATC_y

Cuts at:      0      31     155     605    1566 
   Size:         31     124     450     961

  Fragments arranged by size: 

                 961     450     124      31
 
BtgI C'CryG_G

Cuts at:      0      65    1393    1566 
   Size:         65    1328     173

  Fragments arranged by size: 

                1328     173      65
 
BtsI GCAGTG_nn'

Cuts at:      0     245    1048    1566 
   Size:        245     803     518

  Fragments arranged by size: 

                 803     518     245
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1249    1284    1488    1523    1566 
   Size:       1249      35     204      35      43

  Fragments arranged by size: 

                1249     204      43      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     463    1566 
   Size:        463    1103
 
EaeI y'GGCC_r

Cuts at:      0      62      68     255    1566 
   Size:         62       6     187    1311

  Fragments arranged by size: 

                1311     187      62       6
 
EarI CTCTTCn'nnn_

Cuts at:      0    1097    1334    1566 
   Size:       1097     237     232

  Fragments arranged by size: 

                1097     237     232
 
EcoICRI GAG'CTC

Cuts at:      0     987    1566 
   Size:        987     579
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     556    1168    1334    1566 
   Size:        120     436     612     166     232

  Fragments arranged by size: 

                 612     436     232     166     120
 
HaeII r_GCGC'y

Cuts at:      0      86    1206    1566 
   Size:         86    1120     360

  Fragments arranged by size: 

                1120     360      86
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     369     401     669     701    1373    1405    1566 
   Size:        369      32     268      32     672      32     161

  Fragments arranged by size: 

                 672     369     268     161      32      32      32
 
HincII GTy'rAC

Cuts at:      0     321    1566 
   Size:        321    1245
 
MscI TGG'CCA

Cuts at:      0      64      70    1566 
   Size:         64       6    1496

  Fragments arranged by size: 

                1496      64       6
 
MslI CAynn'nnrTG

Cuts at:      0     811    1041    1566 
   Size:        811     230     525

  Fragments arranged by size: 

                 811     525     230
 
MspA1I CmG'CkG

Cuts at:      0      26    1566 
   Size:         26    1540
 
NcoI C'CATG_G

Cuts at:      0      65    1566 
   Size:         65    1501
 
NsiI A_TGCA'T

Cuts at:      0    1088    1433    1566 
   Size:       1088     345     133

  Fragments arranged by size: 

                1088     345     133
 
NspI r_CATG'y

Cuts at:      0    1173    1566 
   Size:       1173     393
 
PfoI T'CCnGG_A

Cuts at:      0     884     938    1566 
   Size:        884      54     628

  Fragments arranged by size: 

                 884     628      54
 
PvuII CAG'CTG

Cuts at:      0      26    1566 
   Size:         26    1540
 
SacI G_AGCT'C

Cuts at:      0     989    1566 
   Size:        989     577
 
ScaI AGT'ACT

Cuts at:      0     735    1551    1566 
   Size:        735     816      15

  Fragments arranged by size: 

                 816     735      15
 
SfcI C'TryA_G

Cuts at:      0      78     707    1566 
   Size:         78     629     859

  Fragments arranged by size: 

                 859     629      78
 
SmaI CCC'GGG

Cuts at:      0    1155    1566 
   Size:       1155     411
 
SmlI C'TyrA_G

Cuts at:      0     442    1009    1566 
   Size:        442     567     557

  Fragments arranged by size: 

                 567     557     442
 
SphI G_CATG'C

Cuts at:      0    1173    1566 
   Size:       1173     393
 
SspI AAT'ATT

Cuts at:      0     525    1566 
   Size:        525    1041
 
StyI C'CwwG_G

Cuts at:      0      65     547    1267    1566 
   Size:         65     482     720     299

  Fragments arranged by size: 

                 720     482     299      65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      97     811    1566 
   Size:         97     714     755

  Fragments arranged by size: 

                 755     714      97
 
TatI w'GTAC_w

Cuts at:      0     733    1549    1566 
   Size:        733     816      17

  Fragments arranged by size: 

                 816     733      17
 
XbaI T'CTAG_A

Cuts at:      0     696    1566 
   Size:        696     870
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     144     219    1566 
   Size:        144      75    1347

  Fragments arranged by size: 

                1347     144      75
 
XmaI C'CCGG_G

Cuts at:      0    1153    1566 
   Size:       1153     413
 
XmnI GAAnn'nnTTC

Cuts at:      0     893    1566 
   Size:        893     673
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     AlfI    AlwNI     ApoI     AseI     AvaI
    BamHI    BanII     BclI    BfrBI     BlpI     BmrI     BpmI    BpuEI
     BsaI    BsaWI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrDI   BstAPI    BstBI    BstYI     BtgI     BtsI    CspCI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    HaeII    Hin4I   HincII
     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PfoI    PvuII
     SacI     ScaI     SfcI     SmaI     SmlI     SphI     SspI     StyI
    TaqII     TatI     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     StuI     SwaI     TstI  Tth111I     XhoI     ZraI
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