Restriction map for pF1KB5571
 (Linear) (Six-base) MAPSORT of: KIBB5571.seq  Check: 440  from: 1  to: 2754



KIBB5571.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:      0    2344    2754 
   Size:       2344     410
 
Acc65I G'GTAC_C

Cuts at:      0    1423    2754 
   Size:       1423    1331
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     842    2183    2754 
   Size:        842    1341     571

  Fragments arranged by size: 

                1341     842     571
 
AleI CACnn'nnGTG

Cuts at:      0    2174    2754 
   Size:       2174     580
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1556    1588    2754 
   Size:       1556      32    1166

  Fragments arranged by size: 

                1556    1166      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2641    2754 
   Size:       2641     113
 
ApoI r'AATT_y

Cuts at:      0     390    1813    2754 
   Size:        390    1423     941

  Fragments arranged by size: 

                1423     941     390
 
AseI AT'TA_AT

Cuts at:      0    2156    2754 
   Size:       2156     598
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     915     948    2754 
   Size:        915      33    1806

  Fragments arranged by size: 

                1806     915      33
 
BanI G'GyrC_C

Cuts at:      0    1423    2754 
   Size:       1423    1331
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     830    1060    2183    2319    2754 
   Size:        830     230    1123     136     435

  Fragments arranged by size: 

                1123     830     435     230     136
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     916    2754 
   Size:        916    1838
 
BclI T'GATC_A

Cuts at:      0     689    2754 
   Size:        689    2065
 
BfrBI ATG'CAT

Cuts at:      0    2198    2754 
   Size:       2198     556
 
BglII A'GATC_T

Cuts at:      0    2118    2382    2754 
   Size:       2118     264     372

  Fragments arranged by size: 

                2118     372     264
 
BlpI GC'TnA_GC

Cuts at:      0    1878    2754 
   Size:       1878     876
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1986    2754 
   Size:       1986     768
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     359     948     957    1475    2754 
   Size:         89     270     589       9     518    1279

  Fragments arranged by size: 

                1279     589     518     270      89       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1007    1384    1826    2316    2754 
   Size:       1007     377     442     490     438

  Fragments arranged by size: 

                1007     490     442     438     377
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1925    2754 
   Size:       1925     829
 
BsaBI GATnn'nnATC

Cuts at:      0    1119    1184    2754 
   Size:       1119      65    1570

  Fragments arranged by size: 

                1570    1119      65
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1047    1077    1473    1503    2754 
   Size:       1047      30     396      30    1251

  Fragments arranged by size: 

                1251    1047     396      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2000    2383    2754 
   Size:       2000     383     371

  Fragments arranged by size: 

                2000     383     371
 
BseYI C'CCAG_C

Cuts at:      0    2348    2754 
   Size:       2348     406
 
BsiHKAI G_wGCw'C

Cuts at:      0    2266    2754 
   Size:       2266     488
 
BsmI GAATG_Cn'

Cuts at:      0     609    1294    1882    2754 
   Size:        609     685     588     872

  Fragments arranged by size: 

                 872     685     609     588
 
Bsp1286I G_dGCh'C

Cuts at:      0    2266    2754 
   Size:       2266     488
 
BsrBI CCG'CTC

Cuts at:      0    2373    2754 
   Size:       2373     381
 
BsrDI GCAATG_nn'

Cuts at:      0    1057    2754 
   Size:       1057    1697
 
BsrGI T'GTAC_A

Cuts at:      0     111    1038    1760    2754 
   Size:        111     927     722     994

  Fragments arranged by size: 

                 994     927     722     111
 
BssSI C'ACGA_G

Cuts at:      0    1045    2754 
   Size:       1045    1709
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     604    2623    2754 
   Size:        604    2019     131

  Fragments arranged by size: 

                2019     604     131
 
BstEII G'GTnAC_C

Cuts at:      0    1997    2754 
   Size:       1997     757
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     995    2754 
   Size:        995    1759
 
BstYI r'GATC_y

Cuts at:      0      75    1008    1284    1968    2118    2335    2382    2581 
   Size:         75     933     276     684     150     217      47     199

Cuts at:   2581    2754 
   Size:        173

  Fragments arranged by size: 

                 933     684     276     217     199     173     150      75
                  47
 
Bsu36I CC'TnA_GG

Cuts at:      0     224    2754 
   Size:        224    2530
 
BtgI C'CryG_G

Cuts at:      0    2282    2754 
   Size:       2282     472
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1417    2754 
   Size:       1417    1337
 
BtsI GCAGTG_nn'

Cuts at:      0     131    2181    2235    2754 
   Size:        131    2050      54     519

  Fragments arranged by size: 

                2050     519     131      54
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1030    1065    2754 
   Size:       1030      35    1689

  Fragments arranged by size: 

                1689    1030      35
 
DraI TTT'AAA

Cuts at:      0     479    2754 
   Size:        479    2275
 
EaeI y'GGCC_r

Cuts at:      0    2374    2754 
   Size:       2374     380
 
EarI CTCTTCn'nnn_

Cuts at:      0    1904    2754 
   Size:       1904     850
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     659    2754 
   Size:        659    2095
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     359     842     948     957    1475    2183    2754 
   Size:         89     270     483     106       9     518     708     571

  Fragments arranged by size: 

                 708     571     518     483     270     106      89       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     613    1559    2754 
   Size:        613     946    1195

  Fragments arranged by size: 

                1195     946     613
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1564    2754 
   Size:       1564    1190
 
EcoRI G'AATT_C

Cuts at:      0    1813    2754 
   Size:       1813     941
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     691     723     817     849     961     993    1047    1079 
   Size:        691      32      94      32     112      32      54      32

Cuts at:   1079    1152    1184    2110    2142    2544    2576    2754 
   Size:         73      32     926      32     402      32     178

  Fragments arranged by size: 

                 926     691     402     178     112      94      73      54
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0       5     576    2754 
   Size:          5     571    2178

  Fragments arranged by size: 

                2178     571       5
 
KpnI G_GTAC'C

Cuts at:      0    1427    2754 
   Size:       1427    1327
 
MslI CAynn'nnrTG

Cuts at:      0     179    1864    2174    2405    2754 
   Size:        179    1685     310     231     349

  Fragments arranged by size: 

                1685     349     310     231     179
 
MspA1I CmG'CkG

Cuts at:      0    1234    2754 
   Size:       1234    1520
 
NsiI A_TGCA'T

Cuts at:      0    2200    2754 
   Size:       2200     554
 
NspI r_CATG'y

Cuts at:      0    1314    2202    2703    2748    2754 
   Size:       1314     888     501      45       6

  Fragments arranged by size: 

                1314     888     501      45       6
 
PpuMI rG'GwC_Cy

Cuts at:      0    1564    2754 
   Size:       1564    1190
 
PsiI TTA'TAA

Cuts at:      0     757    2754 
   Size:        757    1997
 
PstI C_TGCA'G

Cuts at:      0     138    2458    2619    2754 
   Size:        138    2320     161     135

  Fragments arranged by size: 

                2320     161     138     135
 
PvuII CAG'CTG

Cuts at:      0    1234    2754 
   Size:       1234    1520
 
ScaI AGT'ACT

Cuts at:      0    2440    2754 
   Size:       2440     314
 
SfcI C'TryA_G

Cuts at:      0      59     134    1029    2298    2454    2615    2754 
   Size:         59      75     895    1269     156     161     139

  Fragments arranged by size: 

                1269     895     161     156     139      75      59
 
SmlI C'TyrA_G

Cuts at:      0    1022    1363    1841    2331    2754 
   Size:       1022     341     478     490     423

  Fragments arranged by size: 

                1022     490     478     423     341
 
SpeI A'CTAG_T

Cuts at:      0    1747    2754 
   Size:       1747    1007
 
SphI G_CATG'C

Cuts at:      0    2703    2754 
   Size:       2703      51
 
SspI AAT'ATT

Cuts at:      0     294    2754 
   Size:        294    2460
 
StyI C'CwwG_G

Cuts at:      0     609    1336    2014    2754 
   Size:        609     727     678     740

  Fragments arranged by size: 

                 740     727     678     609
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1308    2754 
   Size:       1308    1446
 
TatI w'GTAC_w

Cuts at:      0     111    1038    1760    2438    2754 
   Size:        111     927     722     678     316

  Fragments arranged by size: 

                 927     722     678     316     111
 
XbaI T'CTAG_A

Cuts at:      0      72    2754 
   Size:         72    2682
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AleI     AloI    AlwNI     ApoI     AseI
     BaeI     BanI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI
     BmrI     BpmI    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    Hin4I   HincII     KpnI     MslI   MspA1I     NsiI     NspI    PpuMI
     PsiI     PstI    PvuII     ScaI     SfcI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI
    BanII     BbeI    BbvCI     BcgI     BglI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BsaAI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrFI   BssHII    BstBI  BstZ17I
     ClaI   DraIII     DrdI     EagI  EcoICRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     StuI     SwaI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
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