Restriction map for pF1KB5556
 (Linear) (Six-base) MAPSORT of: KIBB5556.seq  Check: 4906  from: 1  to: 993



KIBB5556.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     638     993 
   Size:        638     355
 
AccI GT'mk_AC

Cuts at:      0     506     993 
   Size:        506     487
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     459     694     993 
   Size:        166     293     235     299

  Fragments arranged by size: 

                 299     293     235     166
 
AhdI GACnn_n'nnGTC

Cuts at:      0     741     993 
   Size:        741     252
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     672     993 
   Size:        291     381     321

  Fragments arranged by size: 

                 381     321     291
 
AvaI C'yCGr_G

Cuts at:      0     220     697     993 
   Size:        220     477     296

  Fragments arranged by size: 

                 477     296     220
 
BanI G'GyrC_C

Cuts at:      0     558     956     993 
   Size:        558     398      37

  Fragments arranged by size: 

                 558     398      37
 
BbeI G_GCGC'C

Cuts at:      0     562     993 
   Size:        562     431
 
BbvCI CC'TCA_GC

Cuts at:      0     517     993 
   Size:        517     476
 
BglI GCCn_nnn'nGGC

Cuts at:      0     448     583     993 
   Size:        448     135     410

  Fragments arranged by size: 

                 448     410     135
 
BlpI GC'TnA_GC

Cuts at:      0     541     993 
   Size:        541     452
 
Bme1580I G_kGCm'C

Cuts at:      0     260     298     698     993 
   Size:        260      38     400     295

  Fragments arranged by size: 

                 400     295     260      38
 
BmgBI CAC'GTC

Cuts at:      0     195     392     993 
   Size:        195     197     601

  Fragments arranged by size: 

                 601     197     195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     127     279     993 
   Size:        127     152     714

  Fragments arranged by size: 

                 714     152     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     841     859     993 
   Size:        247     594      18     134

  Fragments arranged by size: 

                 594     247     134      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     517     901     993 
   Size:        517     384      92

  Fragments arranged by size: 

                 517     384      92
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     993 
   Size:        190     803
 
BsaHI Gr'CG_yC

Cuts at:      0     214     559     883     993 
   Size:        214     345     324     110

  Fragments arranged by size: 

                 345     324     214     110
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     868     898     993 
   Size:        868      30      95

  Fragments arranged by size: 

                 868      95      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     169     694     993 
   Size:        169     525     299

  Fragments arranged by size: 

                 525     299     169
 
BseYI C'CCAG_C

Cuts at:      0     382     602     635     993 
   Size:        382     220      33     358

  Fragments arranged by size: 

                 382     358     220      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     472     670     993 
   Size:        346     126     198     323

  Fragments arranged by size: 

                 346     323     198     126
 
BsiEI CG_ry'CG

Cuts at:      0     578     993 
   Size:        578     415
 
Bsp1286I G_dGCh'C

Cuts at:      0     260     298     698     993 
   Size:        260      38     400     295

  Fragments arranged by size: 

                 400     295     260      38
 
BspHI T'CATG_A

Cuts at:      0     935     993 
   Size:        935      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     638     993 
   Size:        638     355
 
BsrBI CCG'CTC

Cuts at:      0      68     753     993 
   Size:         68     685     240

  Fragments arranged by size: 

                 685     240      68
 
BsrFI r'CCGG_y

Cuts at:      0     405     501     577     993 
   Size:        405      96      76     416

  Fragments arranged by size: 

                 416     405      96      76
 
BssHII G'CGCG_C

Cuts at:      0     345     536     993 
   Size:        345     191     457

  Fragments arranged by size: 

                 457     345     191
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     630     981     993 
   Size:        630     351      12

  Fragments arranged by size: 

                 630     351      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     738     993 
   Size:        738     255
 
BstYI r'GATC_y

Cuts at:      0      95     993 
   Size:         95     898
 
BtgI C'CryG_G

Cuts at:      0     434     658     887     993 
   Size:        434     224     229     106

  Fragments arranged by size: 

                 434     229     224     106
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     774     993 
   Size:        774     219
 
EaeI y'GGCC_r

Cuts at:      0     125     353     575     890     993 
   Size:        125     228     222     315     103

  Fragments arranged by size: 

                 315     228     222     125     103
 
EagI C'GGCC_G

Cuts at:      0     575     993 
   Size:        575     418
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     778     993 
   Size:        778     215
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     247     459     694     841     859     993 
   Size:        166      81     212     235     147      18     134

  Fragments arranged by size: 

                 235     212     166     147     134      81      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     676     993 
   Size:        676     317
 
EcoO109I rG'GnC_Cy

Cuts at:      0     669     993 
   Size:        669     324
 
FseI GG_CCGG'CC

Cuts at:      0     581     993 
   Size:        581     412
 
HaeII r_GCGC'y

Cuts at:      0     234     482     562     993 
   Size:        234     248      80     431

  Fragments arranged by size: 

                 431     248     234      80
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     727     759     868     900     993 
   Size:        727      32     109      32      93

  Fragments arranged by size: 

                 727     109      93      32      32
 
HincII GTy'rAC

Cuts at:      0     411     948     993 
   Size:        411     537      45

  Fragments arranged by size: 

                 537     411      45
 
HpaI GTT'AAC

Cuts at:      0     948     993 
   Size:        948      45
 
KasI G'GCGC_C

Cuts at:      0     558     993 
   Size:        558     435
 
MslI CAynn'nnrTG

Cuts at:      0     736     993 
   Size:        736     257
 
MspA1I CmG'CkG

Cuts at:      0     436     461     467     548     660     993 
   Size:        436      25       6      81     112     333

  Fragments arranged by size: 

                 436     333     112      81      25       6
 
NaeI GCC'GGC

Cuts at:      0     579     993 
   Size:        579     414
 
NarI GG'CG_CC

Cuts at:      0     559     993 
   Size:        559     434
 
NgoMIV G'CCGG_C

Cuts at:      0     577     993 
   Size:        577     416
 
NspI r_CATG'y

Cuts at:      0     900     993 
   Size:        900      93
 
PstI C_TGCA'G

Cuts at:      0     668     993 
   Size:        668     325
 
SacII CC_GC'GG

Cuts at:      0     437     661     993 
   Size:        437     224     332

  Fragments arranged by size: 

                 437     332     224
 
SfcI C'TryA_G

Cuts at:      0      81     664     993 
   Size:         81     583     329

  Fragments arranged by size: 

                 583     329      81
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     583     993 
   Size:        583     410
 
SfoI GGC'GCC

Cuts at:      0     560     993 
   Size:        560     433
 
SmlI C'TyrA_G

Cuts at:      0     205     993 
   Size:        205     788
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     298     944     993 
   Size:        298     646      49

  Fragments arranged by size: 

                 646     298      49
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     949     993 
   Size:        949      44
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI    AlwNI     AvaI     BanI     BbeI
    BbvCI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstXI    BstYI
     BtgI    BtgZI     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I
     FseI    HaeII    Hin4I   HincII     HpaI     KasI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PstI    SacII     SfcI     SfiI
     SfoI     SmlI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FspI
    FspAI  HindIII     KpnI     MfeI     MluI     MmeI     MscI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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