Restriction map for pF1KB5541
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvrII       
BalI        BbeI        BciVI       BlnI        BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BsrBI       BsrGI       BssHII      BstBI       BstEII      
BstPI       ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       FseI        HaeII       HindIII     
HpaI        KasI        KpnI        MluI        MluNI       
MroI        MscI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    RcaI        RsrII       SalI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SpeI        SspBI       SspI        SwaI        Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AflII        1321  CTTAAG               C^TTAAG
AocI          559  CCTGAGG              CC^T[ACGT]AGG
ApaI          952  GGGCCC               GGGCC^C
AspI          411  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1974  TGCGCA               TGC^GCA
BclI          998  TGATCA               T^GATCA
BfaI         2503  CTAG                 C^TAG
BfrI         1321  CTTAAG               C^TTAAG
BglII        2694  AGATCT               A^GATCT
BlpI         2226  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2226  GCTGAGC              GC^T[ACGT]AGC
BsaBI         720  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        2570  CGATCG               CG[GA][CT]^CG
BsmI          525  GAATGC               GAATGC(1/-1)
BspLU11I      198  ACATGT               A^CATGT
Bst1107I      246  GTATAC               GTA^TAC
BstAPI       1479  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       246  GTATAC               GTA^TAC
Bsu36I        559  CCTGAGG              CC^T[ACGT]AGG
CelII        2226  GCTGAGC              GC^T[ACGT]AGC
DraI          165  TTTAAA               TTT^AAA
EaeI         1787  TGGCCG               [CT]^GGCC[GA]
Eco81I        559  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      1402  ATGCAT               ATGCA^T
FbaI          998  TGATCA               T^GATCA
FspI         1974  TGCGCA               TGC^GCA
KspI          924  CCGCGG               CCGC^GG
MaeI         2503  CTAG                 C^TAG
MamI          720  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1751  CAATTG               C^AATTG
MunI         1751  CAATTG               C^AATTG
NaeI            3  GCCGGC               GCC^GGC
NgoMIV          3  GCCGGC               G^CCGGC
NsiI         1402  ATGCAT               ATGCA^T
PflFI         411  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI         2570  CGATCG               CGAT^CG
SacII         924  CCGCGG               CCGC^GG
SapI          205  GAAGAGC              GCTCTTC(1/4)
SbfI          183  CCTGCAGG             CCTGCA^GG
SexAI        1214  ACCTGGT              A^CC[AT]GGT
SnaBI        2296  TACGTA               TAC^GTA
Sse8387I      183  CCTGCAGG             CCTGCA^GG
StuI          273  AGGCCT               AGG^CCT
TaqI         1925  TCGA                 T^CGA
Tth111I       411  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1925  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          246  GTATAC               GT^[AC][GT]AC
             1113  GTCTAC              
AccII         925  CGCG                 CG^CG
             1780  CGCG                
AcsI         1703  AAATTT               [GA]^AATT[CT]
             2662  AAATTC              
AflIII        198  ACATGT               A^C[GA][CT]GT
             1535  ACACGT              
AlwNI        1459  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1480  CAGCAGCTG           
ApoI         1703  AAATTT               [GA]^AATT[CT]
             2662  AAATTC              
BbrPI        2414  CACGTG               CAC^GTG
             2473  CACGTG              
BbsI         2233  GAAGAC               GAAGAC(2/6)
             2401  GAAGAC              
BbvCI        2462  CCTCAGC              CCTCAGC(-5/-2)
             1741  GCTGAGG             
BglI            3  GCCGGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1627  GCCTCCAAGGC         
BpuAI        2233  GAAGAC               GAAGAC(2/6)
             2401  GAAGAC              
BsaI          742  GAGACC               GGTCTC(1/5)
             2060  GAGACC              
BsaWI         394  ACCGGA               [AT]^CCGG[AT]
              518  TCCGGT              
BsmBI        2801  CGTCTC               CGTCTC(1/5)
             2758  GAGACG              
BspMI        2654  ACCTGC               ACCTGC(4/8)
              186  GCAGGT              
BsrDI        1917  CATTGC               GCAATG(2/0)
             2409  CATTGC              
BsrFI           3  GCCGGC               [GA]^CCGG[CT]
             1901  ACCGGC              
BstUI         925  CGCG                 CG^CG
             1780  CGCG                
Cfr10I          3  GCCGGC               [GA]^CCGG[CT]
             1901  ACCGGC              
DraIII        589  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              789  CACAAAGTG           
HincII        105  GTCAAC               GT[CT]^[GA]AC
             1219  GTTGAC              
HindII        105  GTCAAC               GT[CT]^[GA]AC
             1219  GTTGAC              
MseI          166  TTAA                 T^TAA
             1322  TTAA                
MvnI          925  CGCG                 CG^CG
             1780  CGCG                
NcoI         1734  CCATGG               C^CATGG
             2840  CCATGG              
PmaCI        2414  CACGTG               CAC^GTG
             2473  CACGTG              
PmlI         2414  CACGTG               CAC^GTG
             2473  CACGTG              
PvuII        1483  CAGCTG               CAG^CTG
             1840  CAGCTG              
SmlI         1062  CTTGAG               C^T[CT][GA]AG
             1321  CTTAAG              
SphI          126  GCATGC               GCATG^C
             1400  GCATGC              
Tru9I         166  TTAA                 T^TAA
             1322  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AatII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BamHI       
BanI        BanII       BbvI        BcgI        BcnI        
BmyI        BpmI        BsaAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BssSI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       XcmI        
XhoII       
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