Restriction map for pF1KB5522
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvaII       AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoT22I     FbaI        FseI        
FspI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MscI        MslI        
MunI        NarI        NciI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII         114  CGCG                 CG^CG
AccIII        968  TCCGGA               T^CCGGA
AlwI          971  GGATC                GGATC(4/5)
AlwNI         492  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       422  AGCGCT               AGC^GCT
ApaI            4  GGGCCC               GGGCC^C
AspHI         901  GTGCTC               G[AT]GC[AT]^C
AspI           52  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          993  CCCGAG               C^[CT]CG[GA]G
BanII           4  GGGCCC               G[GA]GC[CT]^C
BglI          799  GCCTGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          181  CTGGAG               CTGGAG(16/14)
BsaWI         968  TCCGGA               [AT]^CCGG[AT]
BseAI         968  TCCGGA               T^CCGGA
BseRI         403  CTCCTC               GAGGAG(10/8)
BsgI          615  CTGCAC               GTGCAG(16/14)
BsiHKAI       901  GTGCTC               G[AT]GC[AT]^C
BsoBI         993  CCCGAG               C^[CT]CG[GA]G
BspEI         968  TCCGGA               T^CCGGA
BspMI         302  ACCTGC               ACCTGC(4/8)
BsrFI        1036  GCCGGC               [GA]^CCGG[CT]
BsrI          345  CCAGT                ACTGG(1/-1)
BstUI         114  CGCG                 CG^CG
Cfr10I       1036  GCCGGC               [GA]^CCGG[CT]
DpnII         972  GATC                 ^GATC
DraII           3  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI         1110  GACCATTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          620  CCACGG               C^C[GA][CT]GG
Eco47III      422  AGCGCT               AGC^GCT
EcoNI         677  CCTTGGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I        3  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1045  GAATTC               G^AATTC
EcoRV          67  GATATC               GAT^ATC
HphI          849  GGTGA                GGTGA(8/7)
MaeIII        860  GTGAC                ^GT[ACGT]AC
MboI          972  GATC                 ^GATC
MroI          968  TCCGGA               T^CCGGA
MseI          134  TTAA                 T^TAA
MvnI          114  CGCG                 CG^CG
NaeI         1036  GCCGGC               GCC^GGC
NdeII         972  GATC                 ^GATC
NgoMIV       1036  GCCGGC               G^CCGGC
PflFI          52  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          663  CTGCAG               CTGCA^G
PvuII         567  CAGCTG               CAG^CTG
SapI          813  GCTCTTC              GCTCTTC(1/4)
Sau3AI        972  GATC                 ^GATC
ScaI          881  AGTACT               AGT^ACT
SfcI          663  CTGCAG               C^T[GA][CT]AG
SmlI          763  CTCAAG               C^T[CT][GA]AG
TfiI          668  GAATC                G^A[AT]TC
Tru9I         134  TTAA                 T^TAA
Tsp45I        860  GTGAC                ^GT[GC]AC
Tth111I        52  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           11  CCAGCGTCCCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BanI          807  GGCACC               G^G[CT][GA]CC
              913  GGCACC              
BsaI         1108  GAGACC               GGTCTC(1/5)
               45  GGTCTC              
BsmFI        1097  GGGAC                GGGAC(10/14)
               16  GTCCC               
EcoT14I       677  CCTTGG               C^C[AT][AT]GG
              725  CCAAGG              
HincII         58  GTCAAC               GT[CT]^[GA]AC
             1132  GTCAAC              
HindII         58  GTCAAC               GT[CT]^[GA]AC
             1132  GTCAAC              
MspA1I         19  CCGCTG               C[AC]G^C[GT]G
              567  CAGCTG              
PleI          687  GACTC                GAGTC(4/5)
             1003  GACTC               
SfaNI         951  GATGC                GCATC(5/9)
              551  GCATC               
StyI          677  CCTTGG               C^C[AT][AT]GG
              725  CCAAGG              
TspRI         346  CAGTG                CA[GC]TG[ACGT][ACGT]^
              555  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbsI        BbvI        BmyI        BpuAI       BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrBI       
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        EarI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
ItaI        Ksp632I     MaeII       MboII       MnlI        
MspI        MvaI        MwoI        NlaIII      NlaIV       
RsaI        Sau96I      ScrFI       TseI        Tsp509I     
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