Restriction map for pF1KB5522
 (Circular) (Six-base) MAPSORT of: pF1KB5522.seq  Check: 4707  from: 1  to: 4259



pF1KB5522  4259 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:25  ..

AccI GT'mk_AC

Cuts at:   1297    1297 
   Size:       4259
 
Acc65I G'GTAC_C

Cuts at:   1275    1275 
   Size:       4259
 
AclI AA'CG_TT

Cuts at:   4099    4099 
   Size:       4259
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    963    2084    2516    2869    3531     963 
   Size:       1121     432     353     662    1691

  Fragments arranged by size: 

                1691    1121     662     432     353
 
AfeI AGC'GCT

Cuts at:    532     532 
   Size:       4259
 
AflIII A'CryG_T

Cuts at:   1611    3411    3692    1611 
   Size:       1800     281    2178

  Fragments arranged by size: 

                2178    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:    605    1564    3002     605 
   Size:        959    1438    1862

  Fragments arranged by size: 

                1862    1438     959
 
ApaI G_GGCC'C

Cuts at:    116     116 
   Size:       4259
 
ApaLI G'TGCA_C

Cuts at:   3097    3097 
   Size:       4259
 
ApoI r'AATT_y

Cuts at:    331    1123    1153    1263    3612    3868     331 
   Size:        792      30     110    2349     256     722

  Fragments arranged by size: 

                2349     792     722     256     110      30
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4259
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4259
 
AvaI C'yCGr_G

Cuts at:   1101    1279    1101 
   Size:        178    4081
 
BamHI G'GATC_C

Cuts at:   1284    1284 
   Size:       4259
 
BanI G'GyrC_C

Cuts at:    915    1021    1275    1689    1937    1972     915 
   Size:        106     254     414     248      35    3202

  Fragments arranged by size: 

                3202     414     254     248     106      35
 
BanII G_rGCy'C

Cuts at:    116    1273    2303     116 
   Size:       1157    1030    2072

  Fragments arranged by size: 

                2072    1157    1030
 
BbeI G_GCGC'C

Cuts at:   1693    1941    1693 
   Size:        248    4011
 
BbsI GAAGACnn'nnnn_

Cuts at:    726     891     967    1220     726 
   Size:        165      76     253    3765

  Fragments arranged by size: 

                3765     253     165      76
 
BciVI GTATCCnnnnn_n'

Cuts at:   2157    3213    4259    2157 
   Size:       1056    1046    2157

  Fragments arranged by size: 

                2157    1056    1046
 
BclI T'GATC_A

Cuts at:   1779    1779 
   Size:       4259
 
BglI GCCn_nnn'nGGC

Cuts at:    913     913 
   Size:       4259
 
BglII A'GATC_T

Cuts at:   1774    3811    1774 
   Size:       2037    2222
 
BlpI GC'TnA_GC

Cuts at:   1371    1371 
   Size:       4259
 
Bme1580I G_kGCm'C

Cuts at:    116     918    1102    1884    1977    3101     116 
   Size:        802     184     782      93    1124    1274

  Fragments arranged by size: 

                1274    1124     802     782     184      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    447    1471    1552    1887    4031     447 
   Size:       1024      81     335    2144     675

  Fragments arranged by size: 

                2144    1024     675     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2626    2626 
   Size:       4259
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    310     310 
   Size:       4259
 
Bpu10I CC'TnA_GC

Cuts at:    892     892 
   Size:       4259
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    856    1435    2314    3028    3326     856 
   Size:        579     879     714     298    1789

  Fragments arranged by size: 

                1789     879     714     579     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    159    1210    3963     159 
   Size:       1051    2753     455

  Fragments arranged by size: 

                2753    1051     455
 
BsaAI yAC'GTr

Cuts at:   1612    1612 
   Size:       4259
 
BsaBI GATnn'nnATC

Cuts at:      6    1773    3810       6 
   Size:       1767    2037     455

  Fragments arranged by size: 

                2037    1767     455
 
BsaHI Gr'CG_yC

Cuts at:   1690    1938    2640    4136    1690 
   Size:        248     702    1496    1813

  Fragments arranged by size: 

                1813    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1076    1448    1969    2647    3058    3205    3479    1076 
   Size:        372     521     678     411     147     274    1856

  Fragments arranged by size: 

                1856     678     521     411     372     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1165    1195    1165 
   Size:         30    4229
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    502     502 
   Size:       4259
 
BseYI C'CCAG_C

Cuts at:    608     911    1684    2442    3107    3515     608 
   Size:        303     773     758     665     408    1352

  Fragments arranged by size: 

                1352     773     758     665     408     303
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    708     708 
   Size:       4259
 
BsiEI CG_ry'CG

Cuts at:    104    1801    1847    3077     104 
   Size:       1697      46    1230    1286

  Fragments arranged by size: 

                1697    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1013    1273    2051    2241    3101    1013 
   Size:        260     778     190     860    2171

  Fragments arranged by size: 

                2171     860     778     260     190
 
Bsp1286I G_dGCh'C

Cuts at:    116     918    1013    1102    1273    1884    1977    2051    2241 
   Size:        802      95      89     171     611      93      74     190

Cuts at:   2241    2303    3101     116 
   Size:         62     798    1274

  Fragments arranged by size: 

                1274     802     798     611     190     171      95      93
                  89      74      62
 
BspEI T'CCGG_A

Cuts at:   1076    1076 
   Size:       4259
 
BspHI T'CATG_A

Cuts at:   2691    2691 
   Size:       4259
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    419    1309    1523    1825    2206     419 
   Size:        890     214     302     381    2472

  Fragments arranged by size: 

                2472     890     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     696     921     985    1106    2551    2605    4089    4254 
   Size:        646     225      64     121    1445      54    1484     165

Cuts at:   4254      50 
   Size:         55

  Fragments arranged by size: 

                1484    1445     646     225     165     121      64      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   2171    2171 
   Size:       4259
 
BsrFI r'CCGG_y

Cuts at:   1144    2257    2438    1144 
   Size:       1113     181    2965

  Fragments arranged by size: 

                2965    1113     181
 
BssSI C'ACGA_G

Cuts at:   2530    3238    2530 
   Size:        708    3551
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1636    1636 
   Size:       4259
 
BstBI TT'CG_AA

Cuts at:   2620    2620 
   Size:       4259
 
BstYI r'GATC_y

Cuts at:      1    1284    1751    1774    2109    2355    2661    2673    2759 
   Size:       1283     467      23     335     246     306      12      86

Cuts at:   2759    3811       1 
   Size:       1052     449

  Fragments arranged by size: 

                1283    1052     467     449     335     306     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     728     107 
   Size:        621    3638
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    504     888    1125    2209    2390    2553     504 
   Size:        384     237    1084     181     163    2210

  Fragments arranged by size: 

                2210    1084     384     237     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1502    1502 
   Size:       4259
 
DraI TTT'AAA

Cuts at:   1258    1258 
   Size:       4259
 
DrdI GACnn_nn'nnGTC

Cuts at:   1224    1965    3309    1224 
   Size:        741    1344    2174

  Fragments arranged by size: 

                2174    1344     741
 
EaeI y'GGCC_r

Cuts at:   1844    2018    2409    2436    3781    1844 
   Size:        174     391      27    1345    2322

  Fragments arranged by size: 

                2322    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1844    1844 
   Size:       4259
 
EarI CTCTTCn'nnn_

Cuts at:    592     724     928     982    1251    2282    2492     592 
   Size:        132     204      54     269    1031     210    2359

  Fragments arranged by size: 

                2359    1031     269     210     204     132      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    571    2467    3209    3355    4069     571 
   Size:       1896     742     146     714     761

  Fragments arranged by size: 

                1896     761     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1271    1271 
   Size:       4259
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    310     963    2084    2516    2869    3531     310 
   Size:        653    1121     432     353     662    1038

  Fragments arranged by size: 

                1121    1038     662     653     432     353
 
EcoNI CCTnn'n_nnAGG

Cuts at:    789     789 
   Size:       4259
 
EcoO109I rG'GnC_Cy

Cuts at:    112    1398     112 
   Size:       1286    2973
 
EcoRI G'AATT_C

Cuts at:   1153    1263    1153 
   Size:        110    4149
 
EcoRV GAT'ATC

Cuts at:    177     177 
   Size:       4259
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2278    2278 
   Size:       4259
 
FspI TGC'GCA

Cuts at:   2040    2040 
   Size:       4259
 
HaeII r_GCGC'y

Cuts at:    534     605     868    1693    1941    3171    3942     534 
   Size:         71     263     825     248    1230     771     851

  Fragments arranged by size: 

                1230     851     825     771     263     248      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1124    1156    1165    1197    2101    2131    2133    2163    1124 
   Size:         32       9      32     904      30       2      30    3220

  Fragments arranged by size: 

                3220     904      32      32      30      30       9       2
            
 
HincII GTy'rAC

Cuts at:    168    1242    1298     168 
   Size:       1074      56    3129

  Fragments arranged by size: 

                3129    1074      56
 
KasI G'GCGC_C

Cuts at:   1689    1937    1689 
   Size:        248    4011
 
KpnI G_GTAC'C

Cuts at:   1279    1279 
   Size:       4259
 
MluI A'CGCG_T

Cuts at:   3692    3692 
   Size:       4259
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3018    3202    3018 
   Size:        184    4075
 
MscI TGG'CCA

Cuts at:   2020    2020 
   Size:       4259
 
MslI CAynn'nnrTG

Cuts at:   2248    2375    2248 
   Size:        127    4132
 
MspA1I CmG'CkG

Cuts at:    129     677    1370    1684    2044    2828    3073     129 
   Size:        548     693     314     360     784     245    1315

  Fragments arranged by size: 

                1315     784     693     548     360     314     245
 
NaeI GCC'GGC

Cuts at:   1146    2440    1146 
   Size:       1294    2965
 
NarI GG'CG_CC

Cuts at:   1690    1938    1690 
   Size:        248    4011
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4259
 
NgoMIV G'CCGG_C

Cuts at:   1144    2438    1144 
   Size:       1294    2965
 
NspI r_CATG'y

Cuts at:   1312    1812    3415    1312 
   Size:        500    1603    2156

  Fragments arranged by size: 

                2156    1603     500
 
PciI A'CATG_T

Cuts at:   3411    3411 
   Size:       4259
 
PfoI T'CCnGG_A

Cuts at:    287     287 
   Size:       4259
 
PmeI GTTT'AAAC

Cuts at:   1258    1258 
   Size:       4259
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2705    2737    2705 
   Size:         32    4227
 
PspOMI G'GGCC_C

Cuts at:    112     112 
   Size:       4259
 
PstI C_TGCA'G

Cuts at:    775    1306    1991     775 
   Size:        531     685    3043

  Fragments arranged by size: 

                3043     685     531
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4259
 
PvuII CAG'CTG

Cuts at:    677    1684    2044     677 
   Size:       1007     360    2892

  Fragments arranged by size: 

                2892    1007     360
 
RsrII CG'GwC_CG

Cuts at:   2454    2454 
   Size:       4259
 
SacI G_AGCT'C

Cuts at:   1273    1273 
   Size:       4259
 
SalI G'TCGA_C

Cuts at:   1296    1296 
   Size:       4259
 
SapI GCTCTTCn'nnn_

Cuts at:    928    2282    2492     928 
   Size:       1354     210    2695

  Fragments arranged by size: 

                2695    1354     210
 
SbfI CC_TGCA'GG

Cuts at:   1306    1306 
   Size:       4259
 
ScaI AGT'ACT

Cuts at:    991    3776     991 
   Size:       2785    1474
 
SfcI C'TryA_G

Cuts at:     33     771    1302    1987    2955    3146      33 
   Size:        738     531     685     968     191    1146

  Fragments arranged by size: 

                1146     968     738     685     531     191
 
SfoI GGC'GCC

Cuts at:   1691    1939    1691 
   Size:        248    4011
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4259
 
SmaI CCC'GGG

Cuts at:   1281    1281 
   Size:       4259
 
SmlI C'TyrA_G

Cuts at:    871    1414    2329    3043    3305     871 
   Size:        543     915     714     262    1825

  Fragments arranged by size: 

                1825     915     714     543     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4259
 
SphI G_CATG'C

Cuts at:   1312    1812    1312 
   Size:        500    3759
 
StyI C'CwwG_G

Cuts at:    107     785     833    1393    1747     107 
   Size:        678      48     560     354    2619

  Fragments arranged by size: 

                2619     678     560     354      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    141     619    1842    1975    2434    2643     141 
   Size:        478    1223     133     459     209    1757

  Fragments arranged by size: 

                1757    1223     478     459     209     133
 
TatI w'GTAC_w

Cuts at:    347     767     989    3774     347 
   Size:        420     222    2785     832

  Fragments arranged by size: 

                2785     832     420     222
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3776    3808    3776 
   Size:         32    4227
 
Tth111I GACn'n_nGTC

Cuts at:    163    2056     163 
   Size:       1893    2366
 
XbaI T'CTAG_A

Cuts at:   1290    1290 
   Size:       4259
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    126    1754     126 
   Size:       1628    2631
 
XmaI C'CCGG_G

Cuts at:   1279    1279 
   Size:       4259
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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