Restriction map for pF1KB5522
 (Linear) (Six-base) MAPSORT of: KIBB5522.seq  Check: 8666  from: 1  to: 1146



KIBB5522.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     855    1146 
   Size:        855     291
 
AfeI AGC'GCT

Cuts at:      0     424    1146 
   Size:        424     722
 
AlwNI CAG_nnn'CTG

Cuts at:      0     497    1146 
   Size:        497     649
 
ApaI G_GGCC'C

Cuts at:      0       8    1146 
   Size:          8    1138
 
ApoI r'AATT_y

Cuts at:      0     223    1015    1045    1146 
   Size:        223     792      30     101

  Fragments arranged by size: 

                 792     223     101      30
 
AvaI C'yCGr_G

Cuts at:      0     993    1146 
   Size:        993     153
 
BanI G'GyrC_C

Cuts at:      0     807     913    1146 
   Size:        807     106     233

  Fragments arranged by size: 

                 807     233     106
 
BanII G_rGCy'C

Cuts at:      0       8    1146 
   Size:          8    1138
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     618     783     859    1112    1146 
   Size:        618     165      76     253      34

  Fragments arranged by size: 

                 618     253     165      76      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     805    1146 
   Size:        805     341
 
Bme1580I G_kGCm'C

Cuts at:      0       8     810     994    1146 
   Size:          8     802     184     152

  Fragments arranged by size: 

                 802     184     152       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     339    1146 
   Size:        339     807
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1146 
   Size:        202     944
 
Bpu10I CC'TnA_GC

Cuts at:      0     784    1146 
   Size:        784     362
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     748    1146 
   Size:        748     398
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      51    1102    1146 
   Size:         51    1051      44

  Fragments arranged by size: 

                1051      51      44
 
BsaWI w'CCGG_w

Cuts at:      0     968    1146 
   Size:        968     178
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1057    1087    1146 
   Size:       1057      30      59

  Fragments arranged by size: 

                1057      59      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     394    1146 
   Size:        394     752
 
BseYI C'CCAG_C

Cuts at:      0     500     803    1146 
   Size:        500     303     343

  Fragments arranged by size: 

                 500     343     303
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     600    1146 
   Size:        600     546
 
BsiHKAI G_wGCw'C

Cuts at:      0     905    1146 
   Size:        905     241
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     810     905     994    1146 
   Size:          8     802      95      89     152

  Fragments arranged by size: 

                 802     152      95      89       8
 
BspEI T'CCGG_A

Cuts at:      0     968    1146 
   Size:        968     178
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     311    1146 
   Size:        311     835
 
BsrBI CCG'CTC

Cuts at:      0     588     813     877     998    1146 
   Size:        588     225      64     121     148

  Fragments arranged by size: 

                 588     225     148     121      64
 
BsrFI r'CCGG_y

Cuts at:      0    1036    1146 
   Size:       1036     110
 
BtgI C'CryG_G

Cuts at:      0     620    1146 
   Size:        620     526
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     396     780    1017    1146 
   Size:        396     384     237     129

  Fragments arranged by size: 

                 396     384     237     129
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1116    1146 
   Size:       1116      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     484     616     820     874    1143    1146 
   Size:        484     132     204      54     269       3

  Fragments arranged by size: 

                 484     269     204     132      54       3
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     463    1146 
   Size:        463     683
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     855    1146 
   Size:        202     653     291

  Fragments arranged by size: 

                 653     291     202
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     681    1146 
   Size:        681     465
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4    1146 
   Size:          4    1142
 
EcoRI G'AATT_C

Cuts at:      0    1045    1146 
   Size:       1045     101
 
EcoRV GAT'ATC

Cuts at:      0      69    1146 
   Size:         69    1077
 
HaeII r_GCGC'y

Cuts at:      0     426     497     760    1146 
   Size:        426      71     263     386

  Fragments arranged by size: 

                 426     386     263      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1016    1048    1057    1089    1146 
   Size:       1016      32       9      32      57

  Fragments arranged by size: 

                1016      57      32      32       9
 
HincII GTy'rAC

Cuts at:      0      60    1134    1146 
   Size:         60    1074      12

  Fragments arranged by size: 

                1074      60      12
 
MspA1I CmG'CkG

Cuts at:      0      21     569    1146 
   Size:         21     548     577

  Fragments arranged by size: 

                 577     548      21
 
NaeI GCC'GGC

Cuts at:      0    1038    1146 
   Size:       1038     108
 
NgoMIV G'CCGG_C

Cuts at:      0    1036    1146 
   Size:       1036     110
 
PfoI T'CCnGG_A

Cuts at:      0     179    1146 
   Size:        179     967
 
PspOMI G'GGCC_C

Cuts at:      0       4    1146 
   Size:          4    1142
 
PstI C_TGCA'G

Cuts at:      0     667    1146 
   Size:        667     479
 
PvuII CAG'CTG

Cuts at:      0     569    1146 
   Size:        569     577
 
SapI GCTCTTCn'nnn_

Cuts at:      0     820    1146 
   Size:        820     326
 
ScaI AGT'ACT

Cuts at:      0     883    1146 
   Size:        883     263
 
SfcI C'TryA_G

Cuts at:      0     663    1146 
   Size:        663     483
 
SmlI C'TyrA_G

Cuts at:      0     763    1146 
   Size:        763     383
 
StyI C'CwwG_G

Cuts at:      0     677     725    1146 
   Size:        677      48     421

  Fragments arranged by size: 

                 677     421      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      33     511    1146 
   Size:         33     478     635

  Fragments arranged by size: 

                 635     478      33
 
TatI w'GTAC_w

Cuts at:      0     239     659     881    1146 
   Size:        239     420     222     265

  Fragments arranged by size: 

                 420     265     239     222
 
Tth111I GACn'n_nGTC

Cuts at:      0      55    1146 
   Size:         55    1091
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      18    1146 
   Size:         18    1128
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BbsI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrBI    BsrFI     BtgI    BtgZI     DrdI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII
   MspA1I     NaeI   NgoMIV     PfoI   PspOMI     PstI    PvuII     SapI
     ScaI     SfcI     SmlI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaBI
    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
  EcoICRI     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI
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