Restriction map for pF1KB5508
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BbrPI       BbsI        
BbvCI       BclI        BfaI        BfrI        BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MaeII       MamI        MfeI        
MluI        MroI        MseI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

BalI         1523  TGGCCA               TGG^CCA
BciVI         467  GTATCC               GTATCC(6/5)
BglI         1610  GCCACAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI         1225  GTGCAG               GTGCAG(16/14)
BsiEI         515  CGGCCG               CG[GA][CT]^CG
BsmAI         673  GAGAC                GTCTC(1/5)
BsmBI         673  GAGACG               CGTCTC(1/5)
BssHII        701  GCGCGC               G^CGCGC
BssSI         787  CTCGTG               CACGAG(-5/-1)
BstXI         176  CCATGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI          515  CGGCCG               C^GGCCG
EclXI         515  CGGCCG               C^GGCCG
Eco52I        515  CGGCCG               C^GGCCG
FseI          189  GGCCGGCC             GGCCGG^CC
HinfI        1023  GATTC                G^A[ACGT]TC
KspI          498  CCGCGG               CCGC^GG
MluNI        1523  TGGCCA               TGG^CCA
MscI         1523  TGGCCA               TGG^CCA
NspI         1496  GCATGC               [GA]CATG^[CT]
PflMI        1415  CCAAGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII         498  CCGCGG               CCGC^GG
SexAI         887  ACCTGGT              A^CC[AT]GGT
SmaI          462  CCCGGG               CCC^GGG
SmlI           73  CTCAAG               C^T[CT][GA]AG
SphI         1496  GCATGC               GCATG^C
TfiI         1023  GATTC                G^A[AT]TC
Van91I       1415  CCAAGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         462  CCCGGG               C^CCGGG
XmaI          462  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          694  GTCTAC               GT^[AC][GT]AC
              754  GTCTAC              
AlwI          639  GATCC                GGATC(4/5)
              993  GATCC               
ApaI          899  GGGCCC               GGGCC^C
             1257  GGGCCC              
AviII         570  TGCGCA               TGC^GCA
              872  TGCGCA              
BbeI          803  GGCGCC               GGCGC^C
             1380  GGCGCC              
BcgI           92  CGAAGCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              332  GCAACGCCCTCG        
BglII         584  AGATCT               A^GATCT
             1364  AGATCT              
BlpI         1398  GCTGAGC              GC^T[ACGT]AGC
             1473  GCTCAGC             
Bpu1102I     1398  GCTGAGC              GC^T[ACGT]AGC
             1473  GCTCAGC             
BspMI         230  ACCTGC               ACCTGC(4/8)
             1574  ACCTGC              
BsrBI        1270  GAGCGG               CCGCTC(-3/-3)
             1401  GAGCGG              
BstEII         60  GGTGACC              G^GT[ACGT]ACC
             1335  GGTGACC             
BstPI          60  GGTGACC              G^GT[ACGT]ACC
             1335  GGTGACC             
CelII        1398  GCTGAGC              GC^T[ACGT]AGC
             1473  GCTCAGC             
EcoNI         939  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1533  CCTCATCGAGG         
EcoO65I        60  GGTGACC              G^GT[ACGT]ACC
             1335  GGTGACC             
FspI          570  TGCGCA               TGC^GCA
              872  TGCGCA              
KasI          803  GGCGCC               G^GCGCC
             1380  GGCGCC              
MaeIII         61  GTGAC                ^GT[ACGT]AC
             1336  GTGAC               
MslI         1212  CACCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1248  CACCCACATG          
NaeI          141  GCCGGC               GCC^GGC
              190  GCCGGC              
NarI          803  GGCGCC               GG^CGCC
             1380  GGCGCC              
NcoI          176  CCATGG               C^CATGG
             1289  CCATGG              
NgoMIV        141  GCCGGC               G^CCGGC
              190  GCCGGC              
SfoI          803  GGCGCC               GGC^GCC
             1380  GGCGCC              
Tsp45I         61  GTGAC                ^GT[GC]AC
             1336  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        Ksp632I     MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        TspRI       TthHB8I     XcmI        
XhoII       
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