Restriction map for pF1KB5508
 (Linear) (Six-base) MAPSORT of: KIBB5508.seq  Check: 41  from: 1  to: 1629



KIBB5508.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AccI GT'mk_AC

Cuts at:      0     695     755    1629 
   Size:        695      60     874

  Fragments arranged by size: 

                 874     695      60
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     961    1141    1629 
   Size:         34     927     180     488

  Fragments arranged by size: 

                 927     488     180      34
 
AleI CACnn'nnGTG

Cuts at:      0    1216    1629 
   Size:       1216     413
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     112    1629 
   Size:        112    1517
 
AlwNI CAG_nnn'CTG

Cuts at:      0     231     435     684     852    1083    1629 
   Size:        231     204     249     168     231     546

  Fragments arranged by size: 

                 546     249     231     231     204     168
 
ApaI G_GGCC'C

Cuts at:      0     903    1261    1629 
   Size:        903     358     368

  Fragments arranged by size: 

                 903     368     358
 
AvaI C'yCGr_G

Cuts at:      0      43     462     739    1629 
   Size:         43     419     277     890

  Fragments arranged by size: 

                 890     419     277      43
 
BanI G'GyrC_C

Cuts at:      0     246     319     478     803    1246    1380    1590    1629 
   Size:        246      73     159     325     443     134     210      39

  Fragments arranged by size: 

                 443     325     246     210     159     134      73      39
            
 
BanII G_rGCy'C

Cuts at:      0     275     903    1261    1312    1621    1629 
   Size:        275     628     358      51     309       8

  Fragments arranged by size: 

                 628     358     309     275      51       8
 
BbeI G_GCGC'C

Cuts at:      0     807    1384    1629 
   Size:        807     577     245

  Fragments arranged by size: 

                 807     577     245
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      81     115     321     355    1629 
   Size:         81      34     206      34    1274

  Fragments arranged by size: 

                1274     206      81      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     478    1629 
   Size:        478    1151
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1616    1629 
   Size:       1616      13
 
BglII A'GATC_T

Cuts at:      0     584    1364    1629 
   Size:        584     780     265

  Fragments arranged by size: 

                 780     584     265
 
BlpI GC'TnA_GC

Cuts at:      0    1399    1474    1629 
   Size:       1399      75     155

  Fragments arranged by size: 

                1399     155      75
 
Bme1580I G_kGCm'C

Cuts at:      0      30     251     322     329     417     736     794     903 
   Size:         30     221      71       7      88     319      58     109

Cuts at:    903    1249    1261    1629 
   Size:        346      12     368

  Fragments arranged by size: 

                 368     346     319     221     109      88      71      58
                  30      12       7
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     972    1326    1629 
   Size:        972     354     303

  Fragments arranged by size: 

                 972     354     303
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     169     256     457     601     838    1156    1225 
   Size:        127      42      87     201     144     237     318      69

Cuts at:   1225    1629 
   Size:        404

  Fragments arranged by size: 

                 404     318     237     201     144     127      87      69
                  42
 
Bpu10I CC'TnA_GC

Cuts at:      0     269    1629 
   Size:        269    1360
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    1629 
   Size:         58    1571
 
BsaHI Gr'CG_yC

Cuts at:      0     804    1381    1608    1629 
   Size:        804     577     227      21

  Fragments arranged by size: 

                 804     577     227      21
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     748     778    1603    1629 
   Size:        748      30     825      26

  Fragments arranged by size: 

                 825     748      30      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     775     835    1213    1318    1629 
   Size:        124     651      60     378     105     311

  Fragments arranged by size: 

                 651     378     311     124     105      60
 
BseYI C'CCAG_C

Cuts at:      0     416     717     776    1455    1629 
   Size:        416     301      59     679     174

  Fragments arranged by size: 

                 679     416     301     174      59
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1246    1629 
   Size:       1246     383
 
BsiEI CG_ry'CG

Cuts at:      0     518    1629 
   Size:        518    1111
 
BsiHKAI G_wGCw'C

Cuts at:      0     116     653    1213    1629 
   Size:        116     537     560     416

  Fragments arranged by size: 

                 560     537     416     116
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     667    1629 
   Size:        667     962
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     116     251     275     322     329     417     653 
   Size:         30      86     135      24      47       7      88     236

Cuts at:    653     736     794     903    1213    1249    1261    1312    1621 
   Size:         83      58     109     310      36      12      51     309

Cuts at:   1621    1629 
   Size:          8

  Fragments arranged by size: 

                 310     309     236     135     109      88      86      83
                  58      51      47      36      30      24      12       8
                   7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     239    1583    1629 
   Size:        239    1344      46

  Fragments arranged by size: 

                1344     239      46
 
BsrBI CCG'CTC

Cuts at:      0    1272    1403    1629 
   Size:       1272     131     226

  Fragments arranged by size: 

                1272     226     131
 
BsrFI r'CCGG_y

Cuts at:      0     141     190     800    1629 
   Size:        141      49     610     829

  Fragments arranged by size: 

                 829     610     141      49
 
BssHII G'CGCG_C

Cuts at:      0     701    1629 
   Size:        701     928
 
BssSI C'ACGA_G

Cuts at:      0     787    1629 
   Size:        787     842
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     246     687    1083    1629 
   Size:        246     441     396     546

  Fragments arranged by size: 

                 546     441     396     246
 
BstEII G'GTnAC_C

Cuts at:      0      60    1335    1629 
   Size:         60    1275     294

  Fragments arranged by size: 

                1275     294      60
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     183    1629 
   Size:        183    1446
 
BstYI r'GATC_y

Cuts at:      0     584     638    1364    1629 
   Size:        584      54     726     265

  Fragments arranged by size: 

                 726     584     265      54
 
BtgI C'CryG_G

Cuts at:      0     176     498    1289    1557    1629 
   Size:        176     322     791     268      72

  Fragments arranged by size: 

                 791     322     268     176      72
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1152    1493    1629 
   Size:       1152     341     136

  Fragments arranged by size: 

                1152     341     136
 
EaeI y'GGCC_r

Cuts at:      0     188     192     293     515    1523    1629 
   Size:        188       4     101     222    1008     106

  Fragments arranged by size: 

                1008     222     188     106     101       4
 
EagI C'GGCC_G

Cuts at:      0     515    1629 
   Size:        515    1114
 
EarI CTCTTCn'nnn_

Cuts at:      0     814     838     889    1505    1629 
   Size:        814      24      51     616     124

  Fragments arranged by size: 

                 814     616     124      51      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1611    1629 
   Size:       1611      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     127     169     256     457     601     838     961 
   Size:         34      93      42      87     201     144     237     123

Cuts at:    961    1141    1156    1225    1629 
   Size:        180      15      69     404

  Fragments arranged by size: 

                 404     237     201     180     144     123      93      87
                  69      42      34      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     943    1537    1629 
   Size:        943     594      92

  Fragments arranged by size: 

                 943     594      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0     128     265     432     828     849     899    1276    1629 
   Size:        128     137     167     396      21      50     377     353

  Fragments arranged by size: 

                 396     377     353     167     137     128      50      21
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     234    1629 
   Size:        234    1395
 
FseI GG_CCGG'CC

Cuts at:      0     194    1629 
   Size:        194    1435
 
FspI TGC'GCA

Cuts at:      0     572     874    1629 
   Size:        572     302     755

  Fragments arranged by size: 

                 755     572     302
 
FspAI rTGC'GCAy

Cuts at:      0     572    1629 
   Size:        572    1057
 
HaeII r_GCGC'y

Cuts at:      0     718     807    1238    1384    1629 
   Size:        718      89     431     146     245

  Fragments arranged by size: 

                 718     431     245     146      89
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     583     615    1629 
   Size:        583      32    1014

  Fragments arranged by size: 

                1014     583      32
 
KasI G'GCGC_C

Cuts at:      0     803    1380    1629 
   Size:        803     577     249

  Fragments arranged by size: 

                 803     577     249
 
MscI TGG'CCA

Cuts at:      0    1525    1629 
   Size:       1525     104
 
MslI CAynn'nnrTG

Cuts at:      0    1216    1252    1629 
   Size:       1216      36     377

  Fragments arranged by size: 

                1216     377      36
 
MspA1I CmG'CkG

Cuts at:      0       8     105     500     684     990    1029    1629 
   Size:          8      97     395     184     306      39     600

  Fragments arranged by size: 

                 600     395     306     184      97      39       8
 
NaeI GCC'GGC

Cuts at:      0     143     192    1629 
   Size:        143      49    1437

  Fragments arranged by size: 

                1437     143      49
 
NarI GG'CG_CC

Cuts at:      0     804    1381    1629 
   Size:        804     577     248

  Fragments arranged by size: 

                 804     577     248
 
NcoI C'CATG_G

Cuts at:      0     176    1289    1629 
   Size:        176    1113     340

  Fragments arranged by size: 

                1113     340     176
 
NgoMIV G'CCGG_C

Cuts at:      0     141     190    1629 
   Size:        141      49    1439

  Fragments arranged by size: 

                1439     141      49
 
NspI r_CATG'y

Cuts at:      0    1500    1629 
   Size:       1500     129
 
PasI CC'CwG_GG

Cuts at:      0    1048    1629 
   Size:       1048     581
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1421    1629 
   Size:       1421     208
 
PfoI T'CCnGG_A

Cuts at:      0     434    1629 
   Size:        434    1195
 
PpuMI rG'GwC_Cy

Cuts at:      0     432     828    1276    1629 
   Size:        432     396     448     353

  Fragments arranged by size: 

                 448     432     396     353
 
PspOMI G'GGCC_C

Cuts at:      0     899    1257    1629 
   Size:        899     358     372

  Fragments arranged by size: 

                 899     372     358
 
PvuII CAG'CTG

Cuts at:      0       8     684     990    1029    1629 
   Size:          8     676     306      39     600

  Fragments arranged by size: 

                 676     600     306      39       8
 
SacII CC_GC'GG

Cuts at:      0     501    1629 
   Size:        501    1128
 
SexAI A'CCwGG_T

Cuts at:      0     887    1629 
   Size:        887     742
 
SfoI GGC'GCC

Cuts at:      0     805    1382    1629 
   Size:        805     577     247

  Fragments arranged by size: 

                 805     577     247
 
SmaI CCC'GGG

Cuts at:      0     464    1629 
   Size:        464    1165
 
SmlI C'TyrA_G

Cuts at:      0      73    1629 
   Size:         73    1556
 
SphI G_CATG'C

Cuts at:      0    1500    1629 
   Size:       1500     129
 
StyI C'CwwG_G

Cuts at:      0     176    1289    1340    1409    1629 
   Size:        176    1113      51      69     220

  Fragments arranged by size: 

                1113     220     176      69      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1264    1320    1629 
   Size:       1264      56     309

  Fragments arranged by size: 

                1264     309      56
 
TatI w'GTAC_w

Cuts at:      0     197    1629 
   Size:        197    1432
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1603    1629 
   Size:       1603      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     183    1422    1452    1629 
   Size:        183    1239      30     177

  Fragments arranged by size: 

                1239     183     177      30
 
XmaI C'CCGG_G

Cuts at:      0     462    1629 
   Size:        462    1167
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
     BplI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI
     FseI     FspI    FspAI    HaeII    Hin4I     KasI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI    PvuII    SacII    SexAI     SfoI     SmaI
     SmlI     SphI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BclI    BfrBI    BmgBI     BmrI
     BmtI     BsaI    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoRI    EcoRV   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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