Restriction map for pF1KB5456
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BamHI       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspEI       
BspHI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      CpoI        
DraI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        FseI        
HgaI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SspBI       SspI        StyI        SwaI        Tru9I       
Tsp509I     Tth111I     XbaI        XhoI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII         438  GACGTC               GACGT^C
AcyI          438  GACGTC               G[GA]^CG[CT]C
AflIII       1236  ACATGT               A^C[GA][CT]GT
AlwI         1055  GGATC                GGATC(4/5)
ApaI          518  GGGCCC               GGGCC^C
Asp700I       267  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         597  GGACC                G^G[AT]CC
AviII         605  TGCGCA               TGC^GCA
BalI          824  TGGCCA               TGG^CCA
BanI            8  GGCACC               G^G[CT][GA]CC
BbvCI         406  GCTGAGG              CCTCAGC(-5/-2)
BglII         783  AGATCT               A^GATCT
BsaHI         438  GACGTC               G[GA]^CG[CT]C
BsaWI         894  TCCGGT               [AT]^CCGG[AT]
BsmFI        1190  GTCCC                GGGAC(10/14)
BspDI         841  ATCGAT               AT^CGAT
BspLU11I     1236  ACATGT               A^CATGT
BsrBI         757  GAGCGG               CCGCTC(-3/-3)
BstF5I        192  CATCC                GGATG(2/0)
BstXI         299  CCAGGAAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         783  AGATCT               [GA]^GATC[CT]
ClaI          841  ATCGAT               AT^CGAT
DraIII        583  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRV         916  GATATC               GAT^ATC
FokI          192  CATCC                GGATG(9/13)
FspI          605  TGCGCA               TGC^GCA
Hin1I         438  GACGTC               G[GA]^CG[CT]C
MaeIII        436  GTGAC                ^GT[ACGT]AC
MflI          783  AGATCT               [GA]^GATC[CT]
MluNI         824  TGGCCA               TGG^CCA
MscI          824  TGGCCA               TGG^CCA
SbfI          984  CCTGCAGG             CCTGCA^GG
SphI          608  GCATGC               GCATG^C
Sse8387I      984  CCTGCAGG             CCTGCA^GG
StuI          416  AGGCCT               AGG^CCT
Tsp45I        436  GTGAC                ^GT[GC]AC
XcmI          299  CCAGGAAACTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         783  AGATCT               [GA]^GATC[CT]
XmnI          267  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI           76  GTAC                 GT^AC
             1017  GTAC                
Aor51HI       155  AGCGCT               AGC^GCT
              221  AGCGCT              
BsmI          496  GAATGC               GAATGC(1/-1)
              770  GAATGC              
BstAPI        357  GCAGGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              582  GCACAGCGTGC         
Eco47III      155  AGCGCT               AGC^GCT
              221  AGCGCT              
MaeII          74  ACGT                 A^CGT
              439  ACGT                
PflMI         501  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              618  CCAGAGCGTGG         
RsaI           76  GTAC                 GT^AC
             1017  GTAC                
SacI          178  GAGCTC               GAGCT^C
              979  GAGCTC              
SapI           18  GAAGAGC              GCTCTTC(1/4)
              708  GCTCTTC             
SfaNI         187  GATGC                GCATC(5/9)
              389  GATGC               
SmaI          521  CCCGGG               CCC^GGG
              953  CCCGGG              
TfiI          171  GAATC                G^A[AT]TC
              210  GATTC               
TspRI          48  CAGTG                CA[GC]TG[ACGT][ACGT]^
              753  CAGTG               
Van91I        501  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              618  CCAGAGCGTGG         
XmaCI         521  CCCGGG               C^CCGGG
              953  CCCGGG              
XmaI          521  CCCGGG               C^CCGGG
              953  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
AvaI        BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfcI        SfiI        
TaqI        TseI        TthHB8I     
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