Restriction map for pF1KB5455
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BanII       
BbrPI       BbvCI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BseAI       BsgI        BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        Eam1105I    Eco47III    EcoNI       
EcoRI       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MaeI        
MfeI        MluI        MroI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SbfI        SexAI       SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccI           28  GTCGAC               GT^[AC][GT]AC
AcyI          107  GGCGCC               G[GA]^CG[CT]C
AocI          772  CCTGAGG              CC^T[ACGT]AGG
AspI          469  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          680  TGGCCA               TGG^CCA
BbeI          107  GGCGCC               GGCGC^C
BbsI           88  GTCTTC               GAAGAC(2/6)
BcgI          498  CGAGGGGTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI           76  GCCCCCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          706  CTGGAG               CTGGAG(16/14)
BpuAI          88  GTCTTC               GAAGAC(2/6)
BsaHI         107  GGCGCC               G[GA]^CG[CT]C
BsaI          373  GAGACC               GGTCTC(1/5)
BsaWI         463  TCCGGT               [AT]^CCGG[AT]
BsiEI         608  CGGCCG               CG[GA][CT]^CG
BsmAI         373  GAGAC                GTCTC(1/5)
BsrFI          55  GCCGGC               [GA]^CCGG[CT]
BstAPI        300  GCACCCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        474  GGTCACC              G^GT[ACGT]ACC
BstPI         474  GGTCACC              G^GT[ACGT]ACC
BstYI         572  AGATCC               [GA]^GATC[CT]
Bsu36I        772  CCTGAGG              CC^T[ACGT]AGG
Cfr10I         55  GCCGGC               [GA]^CCGG[CT]
DrdI          730  GACGGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          608  CGGCCG               C^GGCCG
EclXI         608  CGGCCG               C^GGCCG
Eco52I        608  CGGCCG               C^GGCCG
Eco81I        772  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       474  GGTCACC              G^GT[ACGT]ACC
EcoRV          10  GATATC               GAT^ATC
EcoT14I       335  CCAAGG               C^C[AT][AT]GG
HaeII         107  GGCGCC               [GA]GCGC^[CT]
HgaI          528  GCGTC                GACGC(5/10)
Hin1I         107  GGCGCC               G[GA]^CG[CT]C
HincII         28  GTCGAC               GT[CT]^[GA]AC
HindII         28  GTCGAC               GT[CT]^[GA]AC
KasI          107  GGCGCC               G^GCGCC
MflI          572  AGATCC               [GA]^GATC[CT]
MluNI         680  TGGCCA               TGG^CCA
MscI          680  TGGCCA               TGG^CCA
MseI          635  TTAA                 T^TAA
MspA1I        765  CCGCTG               C[AC]G^C[GT]G
NaeI           55  GCCGGC               GCC^GGC
NarI          107  GGCGCC               GG^CGCC
NgoMIV         55  GCCGGC               G^CCGGC
NspI           44  GCATGT               [GA]CATG^[CT]
PflFI         469  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         548  GGGACCT              [GA]G^G[AT]CC[CT]
SalI           28  GTCGAC               G^TCGAC
SapI          711  GAAGAGC              GCTCTTC(1/4)
ScaI         1007  AGTACT               AGT^ACT
SfcI          591  CTACAG               C^T[GA][CT]AG
SfoI          107  GGCGCC               GGC^GCC
SmaI           80  CCCGGG               CCC^GGG
StyI          335  CCAAGG               C^C[AT][AT]GG
TfiI          151  GATTC                G^A[AT]TC
Tru9I         635  TTAA                 T^TAA
Tth111I       469  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         572  AGATCC               [GA]^GATC[CT]
XmaCI          80  CCCGGG               C^CCGGG
XmaI           80  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI         214  GAGCAC               G[AT]GC[AT]^C
              298  GAGCAC              
AvaI           80  CCCGGG               C^[CT]CG[GA]G
              292  CCCGAG              
AvaII         534  GGACC                G^G[AT]CC
              549  GGACC               
BsaBI         978  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1062  GATGTGGATC          
BsiHKAI       214  GAGCAC               G[AT]GC[AT]^C
              298  GAGCAC              
BsmFI         236  GGGAC                GGGAC(10/14)
              548  GGGAC               
BsoBI          80  CCCGGG               C^[CT]CG[GA]G
              292  CCCGAG              
BspHI         566  TCATGA               T^CATGA
              845  TCATGA              
BsrBI         613  GAGCGG               CCGCTC(-3/-3)
              757  GAGCGG              
BsrI          233  ACTGG                ACTGG(1/-1)
              445  ACTGG               
DdeI          773  CTGAG                C^T[ACGT]AG
              998  CTGAG               
DraII         320  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              548  GGGACCT             
DraIII        301  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
              481  CACACTGTG           
DsaI          605  CCACGG               C^C[GA][CT]GG
              683  CCACGG              
EarI          781  CTCTTC               CTCTTC(1/4)
              711  GAAGAG              
EcoO109I      320  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              548  GGGACCT             
FauI           64  GCGGG                CCCGC(4/6)
              615  GCGGG               
Ksp632I       781  CTCTTC               CTCTTC(1/4)
              711  GAAGAG              
MamI          978  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1062  GATGTGGATC          
MslI          450  CATCGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              906  CACCACCATG          
RcaI          566  TCATGA               T^CATGA
              845  TCATGA              
XcmI          521  CCATCCTGCGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              695  CCAGCTCCTCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
BanI        BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiYI       BslI        Bsp1286I    BsrDI       
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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