Restriction map for pF1KB5455
 (Circular) (Six-base) MAPSORT of: pF1KB5455.seq  Check: 4536  from: 1  to: 4238



pF1KB5455  4238 bp

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:    137    1276     137 
   Size:       1139    3099
 
Acc65I G'GTAC_C

Cuts at:   1254    1254 
   Size:       4238
 
AclI AA'CG_TT

Cuts at:   4078    4078 
   Size:       4238
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    328    2063    2495    3242    3510     328 
   Size:       1735     432     747     268    1056

  Fragments arranged by size: 

                1735    1056     747     432     268
 
AflIII A'CryG_T

Cuts at:   1590    2694    3671    1590 
   Size:       1104     977    2157

  Fragments arranged by size: 

                2157    1104     977
 
AgeI A'CCGG_T

Cuts at:   2626    2626 
   Size:       4238
 
AlwNI CAG_nnn'CTG

Cuts at:   1543    3110    1543 
   Size:       1567    2671
 
ApaLI G'TGCA_C

Cuts at:   3008    3008 
   Size:       4238
 
ApoI r'AATT_y

Cuts at:   1242    3591    3847    1242 
   Size:       2349     256    1633

  Fragments arranged by size: 

                2349    1633     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4238
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4238
 
AvaI C'yCGr_G

Cuts at:    188     400    1258     188 
   Size:        212     858    3168

  Fragments arranged by size: 

                3168     858     212
 
BamHI G'GATC_C

Cuts at:   1263    1263 
   Size:       4238
 
BanI G'GyrC_C

Cuts at:    215     224     365    1012    1068    1254    1668    1916    1951 
   Size:          9     141     647      56     186     414     248      35

Cuts at:   1951     215 
   Size:       2502

  Fragments arranged by size: 

                2502     647     414     248     186     141      56      35
                   9
 
BanII G_rGCy'C

Cuts at:   1252    2282    1252 
   Size:       1030    3208
 
BbeI G_GCGC'C

Cuts at:    219    1672    1920     219 
   Size:       1453     248    2537

  Fragments arranged by size: 

                2537    1453     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    189     189 
   Size:       4238
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    595     629    1212    1246     595 
   Size:         34     583      34    3587

  Fragments arranged by size: 

                3587     583      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2136    2897    4238    2136 
   Size:        761    1341    2136

  Fragments arranged by size: 

                2136    1341     761
 
BclI T'GATC_A

Cuts at:   1758    1758 
   Size:       4238
 
BglI GCCn_nnn'nGGC

Cuts at:    190     190 
   Size:       4238
 
BglII A'GATC_T

Cuts at:   1753    3790    1753 
   Size:       2037    2201
 
BlpI GC'TnA_GC

Cuts at:   1350    1350 
   Size:       4238
 
Bme1580I G_kGCm'C

Cuts at:    599    1863    1956    3012     599 
   Size:       1264      93    1056    1825

  Fragments arranged by size: 

                1825    1264    1056      93
 
BmgBI CAC'GTC

Cuts at:    966     966 
   Size:       4238
 
BmrI ACTGGGnnnn_n'

Cuts at:    351    1450    1531    1866    4010     351 
   Size:       1099      81     335    2144     579

  Fragments arranged by size: 

                2144    1099     579     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    316    2605     316 
   Size:       2289    1949
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    835     835 
   Size:       4238
 
Bpu10I CC'TnA_GC

Cuts at:   1106    1106 
   Size:       4238
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1414    2293    2785    3083    1414 
   Size:        879     492     298    2569

  Fragments arranged by size: 

                2569     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    475    3942     475 
   Size:       3467     771
 
BsaAI yAC'GTr

Cuts at:   1591    1591 
   Size:       4238
 
BsaBI GATnn'nnATC

Cuts at:      6    1090    1174    1752    3789       6 
   Size:       1084      84     578    2037     455

  Fragments arranged by size: 

                2037    1084     578     455      84
 
BsaHI Gr'CG_yC

Cuts at:    216    1669    1917    2619    4115     216 
   Size:       1453     248     702    1496     339

  Fragments arranged by size: 

                1496    1453     702     339     248
 
BsaWI w'CCGG_w

Cuts at:    571    1427    1948    2626    2900    3047    3458     571 
   Size:        856     521     678     274     147     411    1351

  Fragments arranged by size: 

                1351     856     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    130     160     739     769     130 
   Size:         30     579      30    3599

  Fragments arranged by size: 

                3599     579      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    418     798    1091     418 
   Size:        380     293    3565

  Fragments arranged by size: 

                3565     380     293
 
BseYI C'CCAG_C

Cuts at:    495    1072    1663    2421    2998    3494     495 
   Size:        577     591     758     577     496    1239

  Fragments arranged by size: 

                1239     758     591     577     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     719    1780    1826    3034     104 
   Size:        615    1061      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1061     615      46
 
BsiHKAI G_wGCw'C

Cuts at:    326     410    1252    2030    2220    3012     326 
   Size:         84     842     778     190     792    1552

  Fragments arranged by size: 

                1552     842     792     778     190      84
 
Bsp1286I G_dGCh'C

Cuts at:    326     410     599    1252    1863    1956    2030    2220    2282 
   Size:         84     189     653     611      93      74     190      62

Cuts at:   2282    3012     326 
   Size:        730    1552

  Fragments arranged by size: 

                1552     730     653     611     190     189      93      84
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3458    3458 
   Size:       4238
 
BspHI T'CATG_A

Cuts at:    674     953    3414     674 
   Size:        279    2461    1498

  Fragments arranged by size: 

                2461    1498     279
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1288    1502    1804    2185    1288 
   Size:        214     302     381    3341

  Fragments arranged by size: 

                3341     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     723     867    2530    2584    4068    4233      50 
   Size:        673     144    1663      54    1484     165      55

  Fragments arranged by size: 

                1663    1484     673     165     144      55      54
 
BsrDI GCAATG_nn'

Cuts at:    867    1031    1094    2150     867 
   Size:        164      63    1056    2955

  Fragments arranged by size: 

                2955    1056     164      63
 
BsrFI r'CCGG_y

Cuts at:    163    2236    2417    2626     163 
   Size:       2073     181     209    1775

  Fragments arranged by size: 

                2073    1775     209     181
 
BssSI C'ACGA_G

Cuts at:   2509    2867    2509 
   Size:        358    3880
 
BstAPI GCAn_nnn'nTGC

Cuts at:    414    1615     414 
   Size:       1201    3037
 
BstBI TT'CG_AA

Cuts at:   2599    2599 
   Size:       4238
 
BstEII G'GTnAC_C

Cuts at:    582     582 
   Size:       4238
 
BstYI r'GATC_y

Cuts at:      1     680    1263    1730    1753    2088    2334    3346    3432 
   Size:        679     583     467      23     335     246    1012      86

Cuts at:   3432    3444    3790       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     679     583     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    881     881 
   Size:       4238
 
BtgI C'CryG_G

Cuts at:    107     713     791     107 
   Size:        606      78    3554

  Fragments arranged by size: 

                3554     606      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2188    2369    2532    2188 
   Size:        181     163    3894

  Fragments arranged by size: 

                3894     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1058    1481    1058 
   Size:        423    3815
 
DraI TTT'AAA

Cuts at:   1237    1237 
   Size:       4238
 
DraIII CAC_nnn'GTG

Cuts at:    414     594     414 
   Size:        180    4058
 
DrdI GACnn_nn'nnGTC

Cuts at:    844    1944    2802     844 
   Size:       1100     858    2280

  Fragments arranged by size: 

                2280    1100     858
 
EaeI y'GGCC_r

Cuts at:    543     651     716     788     840    1823    1997    2388    2415 
   Size:        108      65      72      52     983     174     391      27

Cuts at:   2415    3760     543 
   Size:       1345    1021

  Fragments arranged by size: 

                1345    1021     983     391     174     108      72      65
                  52      27
 
EagI C'GGCC_G

Cuts at:    716    1823     716 
   Size:       1107    3131
 
EarI CTCTTCn'nnn_

Cuts at:    814     895    2261    2471     814 
   Size:         81    1366     210    2581

  Fragments arranged by size: 

                2581    1366     210      81
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2446    2756    2902    4048    2446 
   Size:        310     146    1146    2636

  Fragments arranged by size: 

                2636    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1250    1250 
   Size:       4238
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    328     835    2063    2495    3242    3510     328 
   Size:        507    1228     432     747     268    1056

  Fragments arranged by size: 

                1228    1056     747     507     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    429     657    1377     429 
   Size:        228     720    3290

  Fragments arranged by size: 

                3290     720     228
 
EcoRI G'AATT_C

Cuts at:   1242    1242 
   Size:       4238
 
EcoRV GAT'ATC

Cuts at:    120     120 
   Size:       4238
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    183    2257     183 
   Size:       2074    2164
 
FspI TGC'GCA

Cuts at:   2019    2019 
   Size:       4238
 
HaeII r_GCGC'y

Cuts at:    219    1672    1920    2942    3921     219 
   Size:       1453     248    1022     979     536

  Fragments arranged by size: 

                1453    1022     979     536     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    130     162    1083    1115    2080    2110    2112    2142     130 
   Size:         32     921      32     965      30       2      30    2226

  Fragments arranged by size: 

                2226     965     921      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    138    1277     138 
   Size:       1139    3099
 
KasI G'GCGC_C

Cuts at:    215    1668    1916     215 
   Size:       1453     248    2537

  Fragments arranged by size: 

                2537    1453     248
 
KpnI G_GTAC'C

Cuts at:   1258    1258 
   Size:       4238
 
MluI A'CGCG_T

Cuts at:   3671    3671 
   Size:       4238
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2909    3093    2909 
   Size:        184    4054
 
MscI TGG'CCA

Cuts at:    790    1999     790 
   Size:       1209    3029
 
MslI CAynn'nnrTG

Cuts at:    562    1018    2227    2354     562 
   Size:        456    1209     127    2446

  Fragments arranged by size: 

                2446    1209     456     127
 
MspA1I CmG'CkG

Cuts at:    875    1349    1663    2023    3036    3281     875 
   Size:        474     314     360    1013     245    1832

  Fragments arranged by size: 

                1832    1013     474     360     314     245
 
NaeI GCC'GGC

Cuts at:    165    2419     165 
   Size:       2254    1984
 
NarI GG'CG_CC

Cuts at:    216    1669    1917     216 
   Size:       1453     248    2537

  Fragments arranged by size: 

                2537    1453     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4238
 
NgoMIV G'CCGG_C

Cuts at:    163    2417     163 
   Size:       2254    1984
 
NspI r_CATG'y

Cuts at:    156    1291    1791    2698     156 
   Size:       1135     500     907    1696

  Fragments arranged by size: 

                1696    1135     907     500
 
PciI A'CATG_T

Cuts at:   2694    2694 
   Size:       4238
 
PmeI GTTT'AAAC

Cuts at:   1237    1237 
   Size:       4238
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3377    3409    3377 
   Size:         32    4206
 
PpuMI rG'GwC_Cy

Cuts at:    657     657 
   Size:       4238
 
PstI C_TGCA'G

Cuts at:   1285    1970    1285 
   Size:        685    3553
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4238
 
PvuII CAG'CTG

Cuts at:   1663    2023    1663 
   Size:        360    3878
 
RsrII CG'GwC_CG

Cuts at:   2433    2433 
   Size:       4238
 
SacI G_AGCT'C

Cuts at:   1252    1252 
   Size:       4238
 
SalI G'TCGA_C

Cuts at:    136    1275     136 
   Size:       1139    3099
 
SapI GCTCTTCn'nnn_

Cuts at:    814    2261    2471     814 
   Size:       1447     210    2581

  Fragments arranged by size: 

                2581    1447     210
 
SbfI CC_TGCA'GG

Cuts at:   1285    1285 
   Size:       4238
 
ScaI AGT'ACT

Cuts at:   1117    3755    1117 
   Size:       2638    1600
 
SfcI C'TryA_G

Cuts at:     33     699    1281    1966    2959    3150      33 
   Size:        666     582     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     666     582     191
 
SfoI GGC'GCC

Cuts at:    217    1670    1918     217 
   Size:       1453     248    2537

  Fragments arranged by size: 

                2537    1453     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4238
 
SmaI CCC'GGG

Cuts at:    190    1260     190 
   Size:       1070    3168
 
SmlI C'TyrA_G

Cuts at:   1393    2308    2800    3062    1393 
   Size:        915     492     262    2569

  Fragments arranged by size: 

                2569     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4238
 
SphI G_CATG'C

Cuts at:   1291    1791    1291 
   Size:        500    3738
 
StyI C'CwwG_G

Cuts at:    107     443    1372    1726     107 
   Size:        336     929     354    2619

  Fragments arranged by size: 

                2619     929     354     336
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    439     601    1086    1821    1954    2413    2622     439 
   Size:        162     485     735     133     459     209    2055

  Fragments arranged by size: 

                2055     735     485     459     209     162     133
 
TatI w'GTAC_w

Cuts at:   1115    3753    1115 
   Size:       2638    1600
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    739     771    3755    3787     739 
   Size:         32    2984      32    1190

  Fragments arranged by size: 

                2984    1190      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    580    2035     580 
   Size:       1455    2783
 
XbaI T'CTAG_A

Cuts at:   1269    1269 
   Size:       4238
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    636     810    1733     636 
   Size:        174     923    3141

  Fragments arranged by size: 

                3141     923     174
 
XmaI C'CCGG_G

Cuts at:    188    1258     188 
   Size:       1070    3168
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI     BmtI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI  BstZ17I     ClaI
    CspCI    EcoNI     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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