Restriction map for pF1KB5432
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       AccI        AccIII      AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HpaI        KasI        
KspI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SwaI        
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I        265  GGTACC               G^GTACC
AccII         739  CGCG                 CG^CG
AclI          386  AACGTT               AA^CGTT
Asp718I       265  GGTACC               G^GTACC
BciVI         197  GTATCC               GTATCC(6/5)
BsgI         1191  GTGCAG               GTGCAG(16/14)
BsmFI         378  GGGAC                GGGAC(10/14)
Bsp1407I      209  TGTACA               T^GTACA
BsrFI         568  GCCGGT               [GA]^CCGG[CT]
BsrGI         209  TGTACA               T^GTACA
BstAPI       1788  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         739  CGCG                 CG^CG
CfoI          740  GCGC                 GCG^C
Cfr10I        568  GCCGGT               [GA]^CCGG[CT]
DraI          295  TTTAAA               TTT^AAA
DraIII        425  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI         344  GAATTC               G^AATTC
EcoRV         655  GATATC               GAT^ATC
FauI         1205  CCCGC                CCCGC(4/6)
HhaI          740  GCGC                 GCG^C
HinP1I        740  GCGC                 G^CGC
HincII        168  GTTGAC               GT[CT]^[GA]AC
HindII        168  GTTGAC               GT[CT]^[GA]AC
HindIII      1402  AAGCTT               A^AGCTT
KpnI          265  GGTACC               GGTAC^C
MspA1I       1297  CAGCTG               C[AC]G^C[GT]G
MvnI          739  CGCG                 CG^CG
NcoI          525  CCATGG               C^CATGG
PpuMI         680  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I      386  AACGTT               AA^CGTT
PstI         1447  CTGCAG               CTGCA^G
PvuII        1297  CAGCTG               CAG^CTG
SacI         1131  GAGCTC               GAGCT^C
SapI         1003  GAAGAGC              GCTCTTC(1/4)
SmlI          841  CTCAAG               C^T[CT][GA]AG
SspBI         209  TGTACA               T^GTACA
TaqI          237  TCGA                 T^CGA
TthHB8I       237  TCGA                 T^CGA
XbaI          348  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         316  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1789  CAGGAGCTG           
AocI          684  CCTCAGG              CC^T[ACGT]AGG
             1083  CCTGAGG             
BanI          265  GGTACC               G^G[CT][GA]CC
             1239  GGTGCC              
BanII        1131  GAGCTC               G[GA]GC[CT]^C
             1554  GGGCTC              
BglII         240  AGATCT               A^GATCT
              959  AGATCT              
BsaBI         235  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1597  GATTACAATC          
BsaI          748  GAGACC               GGTCTC(1/5)
              953  GGTCTC              
BsiYI         360  CCAGTTTAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              893  CCAAGACCCGG         
BslI          360  CCAGTTTAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              893  CCAAGACCCGG         
BspMI         770  GCAGGT               ACCTGC(4/8)
             1371  GCAGGT              
Bsu36I        684  CCTCAGG              CC^T[ACGT]AGG
             1083  CCTGAGG             
DraII         680  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1087  AGGCCCT             
DsaI          408  CCACGG               C^C[GA][CT]GG
              525  CCATGG              
Eco81I        684  CCTCAGG              CC^T[ACGT]AGG
             1083  CCTGAGG             
EcoO109I      680  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1087  AGGCCCT             
MaeII         308  ACGT                 A^CGT
              387  ACGT                
MamI          235  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1597  GATTACAATC          
MslI          526  CATGGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1633  CACGATAATG          
SfcI          648  CTACAG               C^T[GA][CT]AG
             1447  CTGCAG              
SspI           92  AATATT               AAT^ATT
              301  AATATT              
StuI         1472  AGGCCT               AGG^CCT
             1748  AGGCCT              
TfiI          148  GAATC                G^A[AT]TC
              703  GAATC               
Tsp45I       1279  GTGAC                ^GT[GC]AC
             1424  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiHKAI     BsmAI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        Tru9I       
TseI        Tsp509I     TspRI       XhoII       
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