Restriction map for pF1KB5432
 (Circular) (Six-base) MAPSORT of: pF1KB5432.seq  Check: 5843  from: 1  to: 4940



pF1KB5432  4940 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1470    1470 
   Size:       4940
 
AccI GT'mk_AC

Cuts at:   1978    1978 
   Size:       4940
 
Acc65I G'GTAC_C

Cuts at:    373    1956     373 
   Size:       1583    3357
 
AclI AA'CG_TT

Cuts at:    495    4780     495 
   Size:       4285     655
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    742     910    1195    1551    1736    1819    1897    1909    2765 
   Size:        168     285     356     185      83      78      12     856

Cuts at:   2765    3197    3550    4212     742 
   Size:        432     353     662    1470

  Fragments arranged by size: 

                1470     856     662     432     356     353     285     185
                 168      83      78      12
 
AflIII A'CryG_T

Cuts at:   2292    4092    4373    2292 
   Size:       1800     281    2859

  Fragments arranged by size: 

                2859    1800     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1314    1314 
   Size:       4940
 
AlwNI CAG_nnn'CTG

Cuts at:    429    1902    2245    3683     429 
   Size:       1473     343    1438    1686

  Fragments arranged by size: 

                1686    1473    1438     343
 
ApaLI G'TGCA_C

Cuts at:   3778    3778 
   Size:       4940
 
ApoI r'AATT_y

Cuts at:    390     399     452    1379    1944    4293    4549     390 
   Size:          9      53     927     565    2349     256     781

  Fragments arranged by size: 

                2349     927     781     565     256      53       9
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4940
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4940
 
AvaI C'yCGr_G

Cuts at:   1960    1960 
   Size:       4940
 
BamHI G'GATC_C

Cuts at:   1965    1965 
   Size:       4940
 
BanI G'GyrC_C

Cuts at:    373    1347    1956    2370    2618    2653     373 
   Size:        974     609     414     248      35    2660

  Fragments arranged by size: 

                2660     974     609     414     248      35
 
BanII G_rGCy'C

Cuts at:   1243    1666    1954    2984    1243 
   Size:        423     288    1030    3199

  Fragments arranged by size: 

                3199    1030     423     288
 
BbeI G_GCGC'C

Cuts at:   2374    2622    2374 
   Size:        248    4692
 
BbsI GAAGACnn'nnnn_

Cuts at:    629     833    1807     629 
   Size:        204     974    3762

  Fragments arranged by size: 

                3762     974     204
 
BciVI GTATCCnnnnn_n'

Cuts at:    316    2838    3894    4940     316 
   Size:       2522    1056    1046     316

  Fragments arranged by size: 

                2522    1056    1046     316
 
BclI T'GATC_A

Cuts at:   2460    2460 
   Size:       4940
 
BglI GCCn_nnn'nGGC

Cuts at:    244     661    1864     244 
   Size:        417    1203    3320

  Fragments arranged by size: 

                3320    1203     417
 
BglII A'GATC_T

Cuts at:    348    1067    2455    4492     348 
   Size:        719    1388    2037     796

  Fragments arranged by size: 

                2037    1388     796     719
 
BlpI GC'TnA_GC

Cuts at:   2052    2052 
   Size:       4940
 
Bme1580I G_kGCm'C

Cuts at:   1844    2565    2658    3782    1844 
   Size:        721      93    1124    3002

  Fragments arranged by size: 

                3002    1124     721      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1669    2152    2233    2568    4712    1669 
   Size:        483      81     335    2144    1897

  Fragments arranged by size: 

                2144    1897     483     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3307    3307 
   Size:       4940
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1114    1297    1379    1457    1597    1114 
   Size:        183      82      78     140    4457

  Fragments arranged by size: 

                4457     183     140      82      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    934    2116    2995    3709    4007     934 
   Size:       1182     879     714     298    1867

  Fragments arranged by size: 

                1867    1182     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    850    1067    4644     850 
   Size:        217    3577    1146

  Fragments arranged by size: 

                3577    1146     217
 
BsaAI yAC'GTr

Cuts at:   2293    2293 
   Size:       4940
 
BsaBI GATnn'nnATC

Cuts at:      6     347    1709    2454    4491       6 
   Size:        341    1362     745    2037     455

  Fragments arranged by size: 

                2037    1362     745     455     341
 
BsaHI Gr'CG_yC

Cuts at:   2371    2619    3321    4817    2371 
   Size:        248     702    1496    2494

  Fragments arranged by size: 

                2494    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2129    2650    3328    3739    3886    4160    2129 
   Size:        521     678     411     147     274    2909

  Fragments arranged by size: 

                2909     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    947    1234    1382    1807     947 
   Size:        287     148     425    4080

  Fragments arranged by size: 

                4080     425     287     148
 
BseYI C'CCAG_C

Cuts at:   1824    2365    3123    3788    4196    1824 
   Size:        541     758     665     408    2568

  Fragments arranged by size: 

                2568     758     665     541     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1320    1320 
   Size:       4940
 
BsiEI CG_ry'CG

Cuts at:    104    2482    2528    3758     104 
   Size:       2378      46    1230    1286

  Fragments arranged by size: 

                2378    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    873    1243    1260    1954    2732    2922    3782     873 
   Size:        370      17     694     778     190     860    2031

  Fragments arranged by size: 

                2031     860     778     694     370     190      17
 
Bsp1286I G_dGCh'C

Cuts at:    873    1243    1260    1666    1844    1954    2565    2658    2732 
   Size:        370      17     406     178     110     611      93      74

Cuts at:   2732    2922    2984    3782     873 
   Size:        190      62     798    2031

  Fragments arranged by size: 

                2031     798     611     406     370     190     178     110
                  93      74      62      17
 
BspHI T'CATG_A

Cuts at:   3372    3372 
   Size:       4940
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    869    1470    1990    2204    2506    2887     869 
   Size:        601     520     214     302     381    2922

  Fragments arranged by size: 

                2922     601     520     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3232    3286    4770    4935      50 
   Size:       3182      54    1484     165      55

  Fragments arranged by size: 

                3182    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    645    1627    1652    2852     645 
   Size:        982      25    1200    2733

  Fragments arranged by size: 

                2733    1200     982      25
 
BsrFI r'CCGG_y

Cuts at:    676    2938    3119     676 
   Size:       2262     181    2497

  Fragments arranged by size: 

                2497    2262     181
 
BsrGI T'GTAC_A

Cuts at:    317     317 
   Size:       4940
 
BssSI C'ACGA_G

Cuts at:   3211    3919    3211 
   Size:        708    4232
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1902    2317    1902 
   Size:        415    4525
 
BstBI TT'CG_AA

Cuts at:   3301    3301 
   Size:       4940
 
BstYI r'GATC_y

Cuts at:      1     348     520    1067    1965    2432    2455    2790    3036 
   Size:        347     172     547     898     467      23     335     246

Cuts at:   3036    3342    3354    3440    4492       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                1052     898     547     467     449     347     335     306
                 246     172      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    793    1192     793 
   Size:        399    4541
 
BtgI C'CryG_G

Cuts at:    516     633     516 
   Size:        117    4823
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    158    2890    3071    3234     158 
   Size:       2732     181     163    1864

  Fragments arranged by size: 

                2732    1864     181     163
 
BtsI GCAGTG_nn'

Cuts at:    345    1622    2183     345 
   Size:       1277     561    3102

  Fragments arranged by size: 

                3102    1277     561
 
DraI TTT'AAA

Cuts at:    405    1939     405 
   Size:       1534    3406
 
DraIII CAC_nnn'GTG

Cuts at:    538     538 
   Size:       4940
 
DrdI GACnn_nn'nnGTC

Cuts at:   2646    3990    2646 
   Size:       1344    3596
 
EaeI y'GGCC_r

Cuts at:   2525    2699    3090    3117    4462    2525 
   Size:        174     391      27    1345    3003

  Fragments arranged by size: 

                3003    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2525    2525 
   Size:       4940
 
EarI CTCTTCn'nnn_

Cuts at:    164     204    1106    1355    1784    1873    2963    3173     164 
   Size:         40     902     249     429      89    1090     210    1931

  Fragments arranged by size: 

                1931    1090     902     429     249     210      89      40
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3148    3890    4036    4750    3148 
   Size:        742     146     714    3338

  Fragments arranged by size: 

                3338     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1241    1952    1241 
   Size:        711    4229
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    742     910    1114    1195    1297    1379    1457    1551    1597 
   Size:        168     204      81     102      82      78      94      46

Cuts at:   1597    1736    1819    1897    1909    2765    3197    3550    4212 
   Size:        139      83      78      12     856     432     353     662

Cuts at:   4212     742 
   Size:       1470

  Fragments arranged by size: 

                1470     856     662     432     353     204     168     139
                 102      94      83      82      81      78      78      46
                  12
 
EcoO109I rG'GnC_Cy

Cuts at:    789    1196    2079     789 
   Size:        407     883    3650

  Fragments arranged by size: 

                3650     883     407
 
EcoRI G'AATT_C

Cuts at:    452    1944     452 
   Size:       1492    3448
 
EcoRV GAT'ATC

Cuts at:    765     765 
   Size:       4940
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    193    1464    1533    2959     193 
   Size:       1271      69    1426    2174

  Fragments arranged by size: 

                2174    1426    1271      69
 
FspI TGC'GCA

Cuts at:   2721    2721 
   Size:       4940
 
HaeII r_GCGC'y

Cuts at:   2374    2622    3852    4623    2374 
   Size:        248    1230     771    2691

  Fragments arranged by size: 

                2691    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    752     784    2782    2812    2814    2844     752 
   Size:         32    1998      30       2      30    2848

  Fragments arranged by size: 

                2848    1998      32      30      30       2
 
HincII GTy'rAC

Cuts at:    278    1979     278 
   Size:       1701    3239
 
HindIII A'AGCT_T

Cuts at:   1510    1510 
   Size:       4940
 
KasI G'GCGC_C

Cuts at:   2370    2618    2370 
   Size:        248    4692
 
KpnI G_GTAC'C

Cuts at:    377    1960     377 
   Size:       1583    3357
 
MluI A'CGCG_T

Cuts at:   4373    4373 
   Size:       4940
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1346    3699    3883    1346 
   Size:       2353     184    2403

  Fragments arranged by size: 

                2403    2353     184
 
MscI TGG'CCA

Cuts at:   2701    2701 
   Size:       4940
 
MslI CAynn'nnrTG

Cuts at:    638    1745    2929    3056     638 
   Size:       1107    1184     127    2522

  Fragments arranged by size: 

                2522    1184    1107     127
 
MspA1I CmG'CkG

Cuts at:   1407    2051    2365    2725    3509    3754    1407 
   Size:        644     314     360     784     245    2593

  Fragments arranged by size: 

                2593     784     644     360     314     245
 
NaeI GCC'GGC

Cuts at:   3121    3121 
   Size:       4940
 
NarI GG'CG_CC

Cuts at:   2371    2619    2371 
   Size:        248    4692
 
NcoI C'CATG_G

Cuts at:    633     633 
   Size:       4940
 
NgoMIV G'CCGG_C

Cuts at:   3119    3119 
   Size:       4940
 
NspI r_CATG'y

Cuts at:   1993    2493    4096    1993 
   Size:        500    1603    2837

  Fragments arranged by size: 

                2837    1603     500
 
PciI A'CATG_T

Cuts at:   4092    4092 
   Size:       4940
 
PfoI T'CCnGG_A

Cuts at:   1928    1928 
   Size:       4940
 
PmeI GTTT'AAAC

Cuts at:   1939    1939 
   Size:       4940
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3386    3418    3386 
   Size:         32    4908
 
PpuMI rG'GwC_Cy

Cuts at:    789     789 
   Size:       4940
 
PsiI TTA'TAA

Cuts at:    929     929 
   Size:       4940
 
PstI C_TGCA'G

Cuts at:   1559    1987    2672    1559 
   Size:        428     685    3827

  Fragments arranged by size: 

                3827     685     428
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4940
 
PvuII CAG'CTG

Cuts at:   1407    2365    2725    1407 
   Size:        958     360    3622

  Fragments arranged by size: 

                3622     958     360
 
RsrII CG'GwC_CG

Cuts at:   3135    3135 
   Size:       4940
 
SacI G_AGCT'C

Cuts at:   1243    1954    1243 
   Size:        711    4229
 
SalI G'TCGA_C

Cuts at:   1977    1977 
   Size:       4940
 
SapI GCTCTTCn'nnn_

Cuts at:   1106    2963    3173    1106 
   Size:       1857     210    2873

  Fragments arranged by size: 

                2873    1857     210
 
SbfI CC_TGCA'GG

Cuts at:   1987    1987 
   Size:       4940
 
ScaI AGT'ACT

Cuts at:   4457    4457 
   Size:       4940
 
SfcI C'TryA_G

Cuts at:     33     756    1555    1983    2668    3636    3827      33 
   Size:        723     799     428     685     968     191    1146

  Fragments arranged by size: 

                1146     968     799     723     685     428     191
 
SfoI GGC'GCC

Cuts at:   2372    2620    2372 
   Size:        248    4692
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4940
 
SmaI CCC'GGG

Cuts at:   1962    1962 
   Size:       4940
 
SmlI C'TyrA_G

Cuts at:    949    2095    3010    3724    3986     949 
   Size:       1146     915     714     262    1903

  Fragments arranged by size: 

                1903    1146     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4940
 
SphI G_CATG'C

Cuts at:   1993    2493    1993 
   Size:        500    4440
 
SspI AAT'ATT

Cuts at:    202     411     202 
   Size:        209    4731
 
StuI AGG'CCT

Cuts at:   1582    1858    1582 
   Size:        276    4664
 
StyI C'CwwG_G

Cuts at:    242     633     851    1583    1589    1634    1667    1772    2074 
   Size:        391     218     732       6      45      33     105     302

Cuts at:   2074    2428     242 
   Size:        354    2754

  Fragments arranged by size: 

                2754     732     391     354     302     218     105      45
                  33       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2523    2656    3115    3324    2523 
   Size:        133     459     209    4139

  Fragments arranged by size: 

                4139     459     209     133
 
TatI w'GTAC_w

Cuts at:    317    1024    4455     317 
   Size:        707    3431     802

  Fragments arranged by size: 

                3431     802     707
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4457    4489    4457 
   Size:         32    4908
 
Tth111I GACn'n_nGTC

Cuts at:   2737    2737 
   Size:       4940
 
XbaI T'CTAG_A

Cuts at:    456    1971     456 
   Size:       1515    3425
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2435    2435 
   Size:       4940
 
XmaI C'CCGG_G

Cuts at:   1960    1960 
   Size:       4940
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI    PpuMI
     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AleI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BspEI   BssHII   BstEII
    BstXI  BstZ17I     ClaI    CspCI    EcoNI     FseI    FspAI     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmlI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI     XmnI
     ZraI
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