Restriction map for pF1KB5432
 (Linear) (Six-base) MAPSORT of: KIBB5432.seq  Check: 1062  from: 1  to: 1827



KIBB5432.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1362    1827 
   Size:       1362     465
 
Acc65I G'GTAC_C

Cuts at:      0     265    1827 
   Size:        265    1562
 
AclI AA'CG_TT

Cuts at:      0     387    1827 
   Size:        387    1440
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     802    1087    1443    1628    1711    1789    1801 
   Size:        634     168     285     356     185      83      78      12

Cuts at:   1801    1827 
   Size:         26

  Fragments arranged by size: 

                 634     356     285     185     168      83      78      26
                  12
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1206    1827 
   Size:       1206     621
 
AlwNI CAG_nnn'CTG

Cuts at:      0     321    1794    1827 
   Size:        321    1473      33

  Fragments arranged by size: 

                1473     321      33
 
ApoI r'AATT_y

Cuts at:      0     282     291     344    1271    1827 
   Size:        282       9      53     927     556

  Fragments arranged by size: 

                 927     556     282      53       9
 
BanI G'GyrC_C

Cuts at:      0     265    1239    1827 
   Size:        265     974     588

  Fragments arranged by size: 

                 974     588     265
 
BanII G_rGCy'C

Cuts at:      0    1135    1558    1827 
   Size:       1135     423     269

  Fragments arranged by size: 

                1135     423     269
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     521     725    1699    1827 
   Size:        521     204     974     128

  Fragments arranged by size: 

                 974     521     204     128
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     208    1827 
   Size:        208    1619
 
BglI GCCn_nnn'nGGC

Cuts at:      0     136     553    1756    1827 
   Size:        136     417    1203      71

  Fragments arranged by size: 

                1203     417     136      71
 
BglII A'GATC_T

Cuts at:      0     240     959    1827 
   Size:        240     719     868

  Fragments arranged by size: 

                 868     719     240
 
Bme1580I G_kGCm'C

Cuts at:      0    1736    1827 
   Size:       1736      91
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1561    1827 
   Size:       1561     266
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1189    1271    1349    1489    1827 
   Size:       1006     183      82      78     140     338

  Fragments arranged by size: 

                1006     338     183     140      82      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     826    1827 
   Size:        826    1001
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     742     959    1827 
   Size:        742     217     868

  Fragments arranged by size: 

                 868     742     217
 
BsaBI GATnn'nnATC

Cuts at:      0     239    1601    1827 
   Size:        239    1362     226

  Fragments arranged by size: 

                1362     239     226
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     839    1126    1274    1699    1827 
   Size:        839     287     148     425     128

  Fragments arranged by size: 

                 839     425     287     148     128
 
BseYI C'CCAG_C

Cuts at:      0    1716    1827 
   Size:       1716     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1212    1827 
   Size:       1212     615
 
BsiHKAI G_wGCw'C

Cuts at:      0     765    1135    1152    1827 
   Size:        765     370      17     675

  Fragments arranged by size: 

                 765     675     370      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     765    1135    1152    1558    1736    1827 
   Size:        765     370      17     406     178      91

  Fragments arranged by size: 

                 765     406     370     178      91      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     761    1362    1827 
   Size:        761     601     465

  Fragments arranged by size: 

                 761     601     465
 
BsrDI GCAATG_nn'

Cuts at:      0     537    1519    1544    1827 
   Size:        537     982      25     283

  Fragments arranged by size: 

                 982     537     283      25
 
BsrFI r'CCGG_y

Cuts at:      0     568    1827 
   Size:        568    1259
 
BsrGI T'GTAC_A

Cuts at:      0     209    1827 
   Size:        209    1618
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1794    1827 
   Size:       1794      33
 
BstYI r'GATC_y

Cuts at:      0     240     412     959    1827 
   Size:        240     172     547     868

  Fragments arranged by size: 

                 868     547     240     172
 
Bsu36I CC'TnA_GG

Cuts at:      0     685    1084    1827 
   Size:        685     399     743

  Fragments arranged by size: 

                 743     685     399
 
BtgI C'CryG_G

Cuts at:      0     408     525    1827 
   Size:        408     117    1302

  Fragments arranged by size: 

                1302     408     117
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      50    1827 
   Size:         50    1777
 
BtsI GCAGTG_nn'

Cuts at:      0     237    1514    1827 
   Size:        237    1277     313

  Fragments arranged by size: 

                1277     313     237
 
DraI TTT'AAA

Cuts at:      0     297    1827 
   Size:        297    1530
 
DraIII CAC_nnn'GTG

Cuts at:      0     430    1827 
   Size:        430    1397
 
EarI CTCTTCn'nnn_

Cuts at:      0      56      96     998    1247    1676    1765    1827 
   Size:         56      40     902     249     429      89      62

  Fragments arranged by size: 

                 902     429     249      89      62      56      40
 
EcoICRI GAG'CTC

Cuts at:      0    1133    1827 
   Size:       1133     694
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     802    1006    1087    1189    1271    1349    1443 
   Size:        634     168     204      81     102      82      78      94

Cuts at:   1443    1489    1628    1711    1789    1801    1827 
   Size:         46     139      83      78      12      26

  Fragments arranged by size: 

                 634     204     168     139     102      94      83      82
                  81      78      78      46      26      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     681    1088    1827 
   Size:        681     407     739

  Fragments arranged by size: 

                 739     681     407
 
EcoRI G'AATT_C

Cuts at:      0     344    1827 
   Size:        344    1483
 
EcoRV GAT'ATC

Cuts at:      0     657    1827 
   Size:        657    1170
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      85    1356    1425    1827 
   Size:         85    1271      69     402

  Fragments arranged by size: 

                1271     402      85      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     644     676    1827 
   Size:        644      32    1151

  Fragments arranged by size: 

                1151     644      32
 
HincII GTy'rAC

Cuts at:      0     170    1827 
   Size:        170    1657
 
HindIII A'AGCT_T

Cuts at:      0    1402    1827 
   Size:       1402     425
 
KpnI G_GTAC'C

Cuts at:      0     269    1827 
   Size:        269    1558
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1238    1827 
   Size:       1238     589
 
MslI CAynn'nnrTG

Cuts at:      0     530    1637    1827 
   Size:        530    1107     190

  Fragments arranged by size: 

                1107     530     190
 
MspA1I CmG'CkG

Cuts at:      0    1299    1827 
   Size:       1299     528
 
NcoI C'CATG_G

Cuts at:      0     525    1827 
   Size:        525    1302
 
PfoI T'CCnGG_A

Cuts at:      0    1820    1827 
   Size:       1820       7
 
PpuMI rG'GwC_Cy

Cuts at:      0     681    1827 
   Size:        681    1146
 
PsiI TTA'TAA

Cuts at:      0     821    1827 
   Size:        821    1006
 
PstI C_TGCA'G

Cuts at:      0    1451    1827 
   Size:       1451     376
 
PvuII CAG'CTG

Cuts at:      0    1299    1827 
   Size:       1299     528
 
SacI G_AGCT'C

Cuts at:      0    1135    1827 
   Size:       1135     692
 
SapI GCTCTTCn'nnn_

Cuts at:      0     998    1827 
   Size:        998     829
 
SfcI C'TryA_G

Cuts at:      0     648    1447    1827 
   Size:        648     799     380

  Fragments arranged by size: 

                 799     648     380
 
SmlI C'TyrA_G

Cuts at:      0     841    1827 
   Size:        841     986
 
SspI AAT'ATT

Cuts at:      0      94     303    1827 
   Size:         94     209    1524

  Fragments arranged by size: 

                1524     209      94
 
StuI AGG'CCT

Cuts at:      0    1474    1750    1827 
   Size:       1474     276      77

  Fragments arranged by size: 

                1474     276      77
 
StyI C'CwwG_G

Cuts at:      0     134     525     743    1475    1481    1526    1559    1664 
   Size:        134     391     218     732       6      45      33     105

Cuts at:   1664    1827 
   Size:        163

  Fragments arranged by size: 

                 732     391     218     163     134     105      45      33
                   6
 
TatI w'GTAC_w

Cuts at:      0     209     916    1827 
   Size:        209     707     911

  Fragments arranged by size: 

                 911     707     209
 
XbaI T'CTAG_A

Cuts at:      0     348    1827 
   Size:        348    1479
 

 Enzymes that do cut:

     AarI   Acc65I     AclI     AcuI     AlfI    AlwNI     ApoI     BanI
    BanII     BbsI    BciVI     BglI    BglII Bme1580I     BmrI     BpmI
    BpuEI     BsaI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     KpnI     MmeI
     MslI   MspA1I     NcoI     PfoI    PpuMI     PsiI     PstI    PvuII
     SacI     SapI     SfcI     SmlI     SspI     StuI     StyI     TatI
     XbaI

 Enzymes that do not cut: 

    AatII     AccI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I     ClaI    CspCI     DrdI
     EaeI     EagI     EciI    EcoNI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI
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