Restriction map for pF1KB5363
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI          142  GTCTAC               GT^[AC][GT]AC
Asp700I       124  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         327  GGACC                G^G[AT]CC
BbrPI         402  CACGTG               CAC^GTG
BbvI          433  GCAGC                GCAGC(8/12)
BmyI          405  GTGCCC               G[AGT]GC[ACT]^C
BsaAI         402  CACGTG               [CT]AC^GT[GA]
BsiEI          63  CGACCG               CG[GA][CT]^CG
BsiYI          21  CCCCACCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           21  CCCCACCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      405  GTGCCC               G[AGT]GC[ACT]^C
BssSI          49  CACGAG               CACGAG(-5/-1)
Cfr13I        327  GGACC                G^G[ACGT]CC
DraII         326  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      326  GGGACCC              [GA]G^G[ACGT]CC[CT]
HphI          363  TCACC                GGTGA(8/7)
MaeII         403  ACGT                 A^CGT
MspA1I        181  CAGCTG               C[AC]G^C[GT]G
NspI          350  GCATGC               [GA]CATG^[CT]
PmaCI         402  CACGTG               CAC^GTG
PmlI          402  CACGTG               CAC^GTG
PpuMI         326  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         181  CAGCTG               CAG^CTG
Sau96I        327  GGACC                G^G[ACGT]CC
SfaNI         430  GATGC                GCATC(5/9)
SphI          350  GCATGC               GCATG^C
TaqI          336  TCGA                 T^CGA
TseI          433  GCAGC                G^C[AT]GC
TspRI         437  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       336  TCGA                 T^CGA
XmnI          124  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BcnI           66  CCGGG                CC^[GC]GG
              288  CCGGG               
BsaJI          26  CCTCGG               C^C[ACGT][ACGT]GG
              258  CCCTGG              
BsmAI         454  GAGAC                GTCTC(1/5)
               54  GTCTC               
BsmFI          85  GGGAC                GGGAC(10/14)
              326  GGGAC               
BstF5I        446  CATCC                GGATG(2/0)
               30  GGATG               
BstNI         259  CCTGG                CC^[AT]GG
              366  CCTGG               
BstYI         284  AGATCC               [GA]^GATC[CT]
              394  AGATCC              
DdeI           56  CTCAG                C^T[ACGT]AG
              179  CTCAG               
EarI          190  GAAGAG               CTCTTC(1/4)
              263  GAAGAG              
EcoRII        259  CCTGG                ^CC[AT]GG
              366  CCTGG               
FokI          446  CATCC                GGATG(9/13)
               30  GGATG               
HinfI          52  GAGTC                G^A[ACGT]TC
              165  GACTC               
Ksp632I       190  GAAGAG               CTCTTC(1/4)
              263  GAAGAG              
MboII         190  GAAGA                GAAGA(8/7)
              263  GAAGA               
MflI          284  AGATCC               [GA]^GATC[CT]
              394  AGATCC              
MvaI          259  CCTGG                CC^[AT]GG
              366  CCTGG               
NciI           66  CCGGG                CC^[GC]GG
              288  CCGGG               
NheI           70  GCTAGC               G^CTAGC
              171  GCTAGC              
NlaIII        351  CATG                 CATG^
              417  CATG                
PleI          165  GACTC                GAGTC(4/5)
               52  GAGTC               
Tsp509I       201  AATT                 ^AATT
              333  AATT                
XhoII         284  AGATCC               [GA]^GATC[CT]
              394  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AluI        AlwI        BfaI        BsrI        
BssKI       Cac8I       DpnII       Fnu4HI      HapII       
HpaII       ItaI        MaeI        MboI        MnlI        
MspI        MwoI        NdeII       NlaIV       Sau3AI      
ScrFI       
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