Restriction map for pF1KB5341
 (Linear) (Six-base) MAPSORT of: KIBB5341.seq  Check: 7061  from: 1  to: 1038



KIBB5341.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     718    1038 
   Size:        296     422     320

  Fragments arranged by size: 

                 422     320     296
 
AlwNI CAG_nnn'CTG

Cuts at:      0     881    1038 
   Size:        881     157
 
ApaI G_GGCC'C

Cuts at:      0     575    1038 
   Size:        575     463
 
AvaI C'yCGr_G

Cuts at:      0     110    1038 
   Size:        110     928
 
BanII G_rGCy'C

Cuts at:      0     234     297     575    1038 
   Size:        234      63     278     463

  Fragments arranged by size: 

                 463     278     234      63
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     627    1038 
   Size:        627     411
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     481    1038 
   Size:        481     557
 
BglII A'GATC_T

Cuts at:      0     431    1038 
   Size:        431     607
 
Bme1580I G_kGCm'C

Cuts at:      0     575    1038 
   Size:        575     463
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     260     764     847     895    1038 
   Size:        260     504      83      48     143

  Fragments arranged by size: 

                 504     260     143      83      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     200     387     679     690     793    1038 
   Size:        103      97     187     292      11     103     245

  Fragments arranged by size: 

                 292     245     187     103     103      97      11
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     871     901    1038 
   Size:        871      30     137

  Fragments arranged by size: 

                 871     137      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28      52      55     541     959    1038 
   Size:         28      24       3     486     418      79

  Fragments arranged by size: 

                 486     418      79      28      24       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     234     297     737    1038 
   Size:        234      63     440     301

  Fragments arranged by size: 

                 440     301     234      63
 
Bsp1286I G_dGCh'C

Cuts at:      0     234     297     575     737    1038 
   Size:        234      63     278     162     301

  Fragments arranged by size: 

                 301     278     234     162      63
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     863    1038 
   Size:        863     175
 
BsrFI r'CCGG_y

Cuts at:      0      59    1038 
   Size:         59     979
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     691    1038 
   Size:        691     347
 
BstYI r'GATC_y

Cuts at:      0      65     222     431     765    1038 
   Size:         65     157     209     334     273

  Fragments arranged by size: 

                 334     273     209     157      65
 
Bsu36I CC'TnA_GG

Cuts at:      0     863    1038 
   Size:        863     175
 
BtgI C'CryG_G

Cuts at:      0     311     483    1038 
   Size:        311     172     555

  Fragments arranged by size: 

                 555     311     172
 
BtsI GCAGTG_nn'

Cuts at:      0     681    1038 
   Size:        681     357
 
EaeI y'GGCC_r

Cuts at:      0     261    1038 
   Size:        261     777
 
EarI CTCTTCn'nnn_

Cuts at:      0     756    1038 
   Size:        756     282
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58    1038 
   Size:         58     980
 
EcoICRI GAG'CTC

Cuts at:      0     232     295    1038 
   Size:        232      63     743

  Fragments arranged by size: 

                 743     232      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     200     296     387     679     690     718     793 
   Size:        103      97      96      91     292      11      28      75

Cuts at:    793    1038 
   Size:        245

  Fragments arranged by size: 

                 292     245     103      97      96      91      75      28
                  11
 
EcoO109I rG'GnC_Cy

Cuts at:      0     948     963    1038 
   Size:        948      15      75

  Fragments arranged by size: 

                 948      75      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     651    1038 
   Size:        651     387
 
HincII GTy'rAC

Cuts at:      0     426    1038 
   Size:        426     612
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     737     897    1038 
   Size:        737     160     141

  Fragments arranged by size: 

                 737     160     141
 
MspA1I CmG'CkG

Cuts at:      0     960    1038 
   Size:        960      78
 
NcoI C'CATG_G

Cuts at:      0     311     483    1038 
   Size:        311     172     555

  Fragments arranged by size: 

                 555     311     172
 
NdeI CA'TA_TG

Cuts at:      0     783    1038 
   Size:        783     255
 
PfoI T'CCnGG_A

Cuts at:      0     225    1038 
   Size:        225     813
 
PpuMI rG'GwC_Cy

Cuts at:      0     963    1038 
   Size:        963      75
 
PspOMI G'GGCC_C

Cuts at:      0     571    1038 
   Size:        571     467
 
SacI G_AGCT'C

Cuts at:      0     234     297    1038 
   Size:        234      63     741

  Fragments arranged by size: 

                 741     234      63
 
SanDI GG'GwC_CC

Cuts at:      0     963    1038 
   Size:        963      75
 
StyI C'CwwG_G

Cuts at:      0     311     483     555    1026    1038 
   Size:        311     172      72     471      12

  Fragments arranged by size: 

                 471     311     172      72      12
 
Tth111I GACn'n_nGTC

Cuts at:      0     871    1038 
   Size:        871     167
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     AvaI    BanII     BbsI    BciVI    BglII
 Bme1580I     BmrI     BpmI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspMI
    BsrFI   BstAPI    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI   HincII     MmeI   MspA1I
     NcoI     NdeI     PfoI    PpuMI   PspOMI     SacI    SanDI     StyI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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