Restriction map for pF1KB5302
 (Linear) (Six-base) MAPSORT of: KIBB5302.seq  Check: 3468  from: 1  to: 927



KIBB5302.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AccI GT'mk_AC

Cuts at:      0     495     927 
   Size:        495     432
 
AflII C'TTAA_G

Cuts at:      0     307     927 
   Size:        307     620
 
AflIII A'CryG_T

Cuts at:      0     894     927 
   Size:        894      33
 
AleI CACnn'nnGTG

Cuts at:      0     706     927 
   Size:        706     221
 
AlwNI CAG_nnn'CTG

Cuts at:      0     157     927 
   Size:        157     770
 
AvaI C'yCGr_G

Cuts at:      0     638     927 
   Size:        638     289
 
BanI G'GyrC_C

Cuts at:      0     749     927 
   Size:        749     178
 
BanII G_rGCy'C

Cuts at:      0     720     927 
   Size:        720     207
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     124     158     927 
   Size:        124      34     769

  Fragments arranged by size: 

                 769     124      34
 
BglII A'GATC_T

Cuts at:      0     441     485     927 
   Size:        441      44     442

  Fragments arranged by size: 

                 442     441      44
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     511     927 
   Size:        511     416
 
BsaWI w'CCGG_w

Cuts at:      0     758     927 
   Size:        758     169
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     242     272     927 
   Size:        242      30     655

  Fragments arranged by size: 

                 655     242      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     626     656     927 
   Size:        626      30     271

  Fragments arranged by size: 

                 626     271      30
 
BseYI C'CCAG_C

Cuts at:      0     870     927 
   Size:        870      57
 
BsiHKAI G_wGCw'C

Cuts at:      0     546     720     786     927 
   Size:        546     174      66     141

  Fragments arranged by size: 

                 546     174     141      66
 
Bsp1286I G_dGCh'C

Cuts at:      0     546     720     786     927 
   Size:        546     174      66     141

  Fragments arranged by size: 

                 546     174     141      66
 
BspHI T'CATG_A

Cuts at:      0     186     351     927 
   Size:        186     165     576

  Fragments arranged by size: 

                 576     186     165
 
BstEII G'GTnAC_C

Cuts at:      0     808     927 
   Size:        808     119
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      27     927 
   Size:         27     900
 
BstYI r'GATC_y

Cuts at:      0     441     485     603     927 
   Size:        441      44     118     324

  Fragments arranged by size: 

                 441     324     118      44
 
BtgI C'CryG_G

Cuts at:      0     615     927 
   Size:        615     312
 
BtsI GCAGTG_nn'

Cuts at:      0     292     464     927 
   Size:        292     172     463

  Fragments arranged by size: 

                 463     292     172
 
EcoICRI GAG'CTC

Cuts at:      0     718     927 
   Size:        718     209
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     881     927 
   Size:        881      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     477     509     669     701     927 
   Size:        477      32     160      32     226

  Fragments arranged by size: 

                 477     226     160      32      32
 
HincII GTy'rAC

Cuts at:      0     261     927 
   Size:        261     666
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     927 
   Size:        152     775
 
MslI CAynn'nnrTG

Cuts at:      0     692     706     927 
   Size:        692      14     221

  Fragments arranged by size: 

                 692     221      14
 
MspA1I CmG'CkG

Cuts at:      0      48      66     927 
   Size:         48      18     861

  Fragments arranged by size: 

                 861      48      18
 
NspI r_CATG'y

Cuts at:      0     898     927 
   Size:        898      29
 
PciI A'CATG_T

Cuts at:      0     894     927 
   Size:        894      33
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      30     927 
   Size:         30     897
 
PspXI vC'TCGA_Gb

Cuts at:      0     638     927 
   Size:        638     289
 
PvuII CAG'CTG

Cuts at:      0      48      66     927 
   Size:         48      18     861

  Fragments arranged by size: 

                 861      48      18
 
SacI G_AGCT'C

Cuts at:      0     720     927 
   Size:        720     207
 
SfcI C'TryA_G

Cuts at:      0     518     927 
   Size:        518     409
 
SmlI C'TyrA_G

Cuts at:      0     307     638     927 
   Size:        307     331     289

  Fragments arranged by size: 

                 331     307     289
 
SspI AAT'ATT

Cuts at:      0     433     927 
   Size:        433     494
 
StyI C'CwwG_G

Cuts at:      0      20     470     927 
   Size:         20     450     457

  Fragments arranged by size: 

                 457     450      20
 
XbaI T'CTAG_A

Cuts at:      0     712     927 
   Size:        712     215
 
XhoI C'TCGA_G

Cuts at:      0     638     927 
   Size:        638     289
 
XmnI GAAnn'nnTTC

Cuts at:      0      13     927 
   Size:         13     914
 

 Enzymes that do cut:

     AccI    AflII   AflIII     AleI    AlwNI     AvaI     BanI    BanII
     BcgI    BglII     BsaI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspHI   BstEII    BstXI    BstYI     BtgI     BtsI  EcoICRI
    EcoNI    Hin4I   HincII     MmeI     MslI   MspA1I     NspI     PciI
     PpiI    PspXI    PvuII     SacI     SfcI     SmlI     SspI     StyI
     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     ZraI
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