Restriction map for pF1KB5274
 (Linear) (Six-base) MAPSORT of: KIBB5274.seq  Check: 9880  from: 1  to: 1047



KIBB5274.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AccI GT'mk_AC

Cuts at:      0     203    1047 
   Size:        203     844
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542    1047 
   Size:        542     505
 
AlwNI CAG_nnn'CTG

Cuts at:      0     520    1047 
   Size:        520     527
 
ApoI r'AATT_y

Cuts at:      0     839    1047 
   Size:        839     208
 
BanII G_rGCy'C

Cuts at:      0     974    1047 
   Size:        974      73
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     454     992    1047 
   Size:        454     538      55

  Fragments arranged by size: 

                 538     454      55
 
BbvCI CC'TCA_GC

Cuts at:      0     676    1047 
   Size:        676     371
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     297    1047 
   Size:        297     750
 
BglII A'GATC_T

Cuts at:      0     753    1047 
   Size:        753     294
 
BmtI G_CTAG'C

Cuts at:      0     338    1047 
   Size:        338     709
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47      55     461     587     778    1047 
   Size:         47       8     406     126     191     269

  Fragments arranged by size: 

                 406     269     191     126      47       8
 
Bpu10I CC'TnA_GC

Cuts at:      0     676    1047 
   Size:        676     371
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     925    1047 
   Size:        100     825     122

  Fragments arranged by size: 

                 825     122     100
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     697     874    1047 
   Size:        697     177     173

  Fragments arranged by size: 

                 697     177     173
 
BsaBI GATnn'nnATC

Cuts at:      0     302     308    1047 
   Size:        302       6     739

  Fragments arranged by size: 

                 739     302       6
 
BsaHI Gr'CG_yC

Cuts at:      0     153    1047 
   Size:        153     894
 
BsaWI w'CCGG_w

Cuts at:      0     443    1047 
   Size:        443     604
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      58    1047 
   Size:         28      30     989

  Fragments arranged by size: 

                 989      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      14     337    1036    1047 
   Size:         14     323     699      11

  Fragments arranged by size: 

                 699     323      14      11
 
BseYI C'CCAG_C

Cuts at:      0     707    1047 
   Size:        707     340
 
BsiHKAI G_wGCw'C

Cuts at:      0     974    1047 
   Size:        974      73
 
Bsp1286I G_dGCh'C

Cuts at:      0     974    1047 
   Size:        974      73
 
BspEI T'CCGG_A

Cuts at:      0     443    1047 
   Size:        443     604
 
BspHI T'CATG_A

Cuts at:      0     917    1047 
   Size:        917     130
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     215    1047 
   Size:        215     832
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     828    1047 
   Size:        828     219
 
BstYI r'GATC_y

Cuts at:      0     753     909    1047 
   Size:        753     156     138

  Fragments arranged by size: 

                 753     156     138
 
BtgI C'CryG_G

Cuts at:      0     399    1047 
   Size:        399     648
 
BtsI GCAGTG_nn'

Cuts at:      0     693    1047 
   Size:        693     354
 
EarI CTCTTCn'nnn_

Cuts at:      0     427     603    1047 
   Size:        427     176     444

  Fragments arranged by size: 

                 444     427     176
 
EcoICRI GAG'CTC

Cuts at:      0     972    1047 
   Size:        972      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47      55     461     542     587     778    1047 
   Size:         47       8     406      81      45     191     269

  Fragments arranged by size: 

                 406     269     191      81      47      45       8
 
EcoRI G'AATT_C

Cuts at:      0     839    1047 
   Size:        839     208
 
EcoRV GAT'ATC

Cuts at:      0     270     594    1047 
   Size:        270     324     453

  Fragments arranged by size: 

                 453     324     270
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     696     728    1015    1047 
   Size:        696      32     287      32

  Fragments arranged by size: 

                 696     287      32      32
 
HincII GTy'rAC

Cuts at:      0     111    1047 
   Size:        111     936
 
HindIII A'AGCT_T

Cuts at:      0     225    1047 
   Size:        225     822
 
MfeI C'AATT_G

Cuts at:      0     131    1047 
   Size:        131     916
 
MslI CAynn'nnrTG

Cuts at:      0     146     716     922    1047 
   Size:        146     570     206     125

  Fragments arranged by size: 

                 570     206     146     125
 
MspA1I CmG'CkG

Cuts at:      0     265     828    1047 
   Size:        265     563     219

  Fragments arranged by size: 

                 563     265     219
 
NheI G'CTAG_C

Cuts at:      0     334    1047 
   Size:        334     713
 
PfoI T'CCnGG_A

Cuts at:      0     603    1047 
   Size:        603     444
 
PvuII CAG'CTG

Cuts at:      0     265     828    1047 
   Size:        265     563     219

  Fragments arranged by size: 

                 563     265     219
 
SacI G_AGCT'C

Cuts at:      0     974    1047 
   Size:        974      73
 
ScaI AGT'ACT

Cuts at:      0     534    1047 
   Size:        534     513
 
SfcI C'TryA_G

Cuts at:      0     769    1047 
   Size:        769     278
 
SmlI C'TyrA_G

Cuts at:      0     115     940    1047 
   Size:        115     825     107

  Fragments arranged by size: 

                 825     115     107
 
StuI AGG'CCT

Cuts at:      0     587    1047 
   Size:        587     460
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1004    1047 
   Size:       1004      43
 
TatI w'GTAC_w

Cuts at:      0      93     511     532     960    1047 
   Size:         93     418      21     428      87

  Fragments arranged by size: 

                 428     418      93      87      21
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BanII     BbsI    BbvCI    BciVI
    BglII     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BstXI    BstYI     BtgI     BtsI     EarI  EcoICRI  Eco57MI
    EcoRI    EcoRV    Hin4I   HincII  HindIII     MfeI     MslI   MspA1I
     NheI     PfoI    PvuII     SacI     ScaI     SfcI     SmlI     StuI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI
    BsaAI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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