Restriction map for pF1KB5234
 (Linear) (Six-base) MAPSORT of: KIBB5234.seq  Check: 8169  from: 1  to: 1602



KIBB5234.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AccI GT'mk_AC

Cuts at:      0     466    1602 
   Size:        466    1136
 
Acc65I G'GTAC_C

Cuts at:      0     860    1602 
   Size:        860     742
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1602 
   Size:        503    1099
 
AfeI AGC'GCT

Cuts at:      0     538    1063    1602 
   Size:        538     525     539

  Fragments arranged by size: 

                 539     538     525
 
AflIII A'CryG_T

Cuts at:      0    1463    1602 
   Size:       1463     139
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1515    1602 
   Size:       1515      87
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     706    1602 
   Size:        706     896
 
AlwNI CAG_nnn'CTG

Cuts at:      0     178     427    1134    1275    1602 
   Size:        178     249     707     141     327

  Fragments arranged by size: 

                 707     327     249     178     141
 
ApaI G_GGCC'C

Cuts at:      0     291     606    1602 
   Size:        291     315     996

  Fragments arranged by size: 

                 996     315     291
 
ApaLI G'TGCA_C

Cuts at:      0     982    1602 
   Size:        982     620
 
AvaI C'yCGr_G

Cuts at:      0     380     644    1406    1602 
   Size:        380     264     762     196

  Fragments arranged by size: 

                 762     380     264     196
 
BanI G'GyrC_C

Cuts at:      0      23     860    1602 
   Size:         23     837     742

  Fragments arranged by size: 

                 837     742      23
 
BanII G_rGCy'C

Cuts at:      0      16     291     413     606    1415    1602 
   Size:         16     275     122     193     809     187

  Fragments arranged by size: 

                 809     275     193     187     122      16
 
BbeI G_GCGC'C

Cuts at:      0      27    1602 
   Size:         27    1575
 
BbvCI CC'TCA_GC

Cuts at:      0     950    1602 
   Size:        950     652
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     261     295    1602 
   Size:        261      34    1307

  Fragments arranged by size: 

                1307     261      34
 
BclI T'GATC_A

Cuts at:      0    1226    1602 
   Size:       1226     376
 
BglII A'GATC_T

Cuts at:      0     557    1602 
   Size:        557    1045
 
BlpI GC'TnA_GC

Cuts at:      0     179    1602 
   Size:        179    1423
 
Bme1580I G_kGCm'C

Cuts at:      0     291     606     986    1602 
   Size:        291     315     380     616

  Fragments arranged by size: 

                 616     380     315     291
 
BmtI G_CTAG'C

Cuts at:      0    1423    1602 
   Size:       1423     179
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1505    1602 
   Size:       1505      97
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1602 
   Size:        919     683
 
Bpu10I CC'TnA_GC

Cuts at:      0     950    1602 
   Size:        950     652
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1042    1076    1602 
   Size:       1042      34     526

  Fragments arranged by size: 

                1042     526      34
 
BsaAI yAC'GTr

Cuts at:      0     234    1602 
   Size:        234    1368
 
BsaBI GATnn'nnATC

Cuts at:      0    1393    1602 
   Size:       1393     209
 
BsaHI Gr'CG_yC

Cuts at:      0      24    1602 
   Size:         24    1578
 
BsaWI w'CCGG_w

Cuts at:      0     704    1602 
   Size:        704     898
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      77     130     496    1602 
   Size:         77      53     366    1106

  Fragments arranged by size: 

                1106     366      77      53
 
BseYI C'CCAG_C

Cuts at:      0     188    1014    1415    1602 
   Size:        188     826     401     187

  Fragments arranged by size: 

                 826     401     188     187
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370    1602 
   Size:        370    1232
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     233     986    1602 
   Size:        185      48     753     616

  Fragments arranged by size: 

                 753     616     185      48
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     185     233     291     413     606     986    1415 
   Size:         16     169      48      58     122     193     380     429

Cuts at:   1415    1602 
   Size:        187

  Fragments arranged by size: 

                 429     380     193     187     169     122      58      48
                  16
 
BspEI T'CCGG_A

Cuts at:      0     704    1602 
   Size:        704     898
 
BspHI T'CATG_A

Cuts at:      0     521    1602 
   Size:        521    1081
 
BsrFI r'CCGG_y

Cuts at:      0      87    1031    1602 
   Size:         87     944     571

  Fragments arranged by size: 

                 944     571      87
 
BssSI C'ACGA_G

Cuts at:      0     508    1357    1602 
   Size:        508     849     245

  Fragments arranged by size: 

                 849     508     245
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     897    1572    1602 
   Size:        897     675      30

  Fragments arranged by size: 

                 897     675      30
 
BstYI r'GATC_y

Cuts at:      0     557    1022    1602 
   Size:        557     465     580

  Fragments arranged by size: 

                 580     557     465
 
BtgI C'CryG_G

Cuts at:      0      27     413    1602 
   Size:         27     386    1189

  Fragments arranged by size: 

                1189     386      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1248    1602 
   Size:       1248     354
 
DraIII CAC_nnn'GTG

Cuts at:      0    1203    1602 
   Size:       1203     399
 
EarI CTCTTCn'nnn_

Cuts at:      0     401    1602 
   Size:        401    1201
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      74    1033    1602 
   Size:         74     959     569

  Fragments arranged by size: 

                 959     569      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     919    1602 
   Size:        503     416     683

  Fragments arranged by size: 

                 683     503     416
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     940    1602 
   Size:        940     662
 
EcoO109I rG'GnC_Cy

Cuts at:      0     288     603     945    1131    1169    1602 
   Size:        288     315     342     186      38     433

  Fragments arranged by size: 

                 433     342     315     288     186      38
 
EcoRV GAT'ATC

Cuts at:      0    1455    1602 
   Size:       1455     147
 
FspI TGC'GCA

Cuts at:      0     682    1602 
   Size:        682     920
 
HaeII r_GCGC'y

Cuts at:      0      27     540    1065    1602 
   Size:         27     513     525     537

  Fragments arranged by size: 

                 537     525     513      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     573     605    1501    1533    1602 
   Size:        573      32     896      32      69

  Fragments arranged by size: 

                 896     573      69      32      32
 
HincII GTy'rAC

Cuts at:      0     140     467    1602 
   Size:        140     327    1135

  Fragments arranged by size: 

                1135     327     140
 
HindIII A'AGCT_T

Cuts at:      0     988    1602 
   Size:        988     614
 
KasI G'GCGC_C

Cuts at:      0      23    1602 
   Size:         23    1579
 
KpnI G_GTAC'C

Cuts at:      0     864    1602 
   Size:        864     738
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     525    1262    1602 
   Size:        525     737     340

  Fragments arranged by size: 

                 737     525     340
 
MslI CAynn'nnrTG

Cuts at:      0     520    1000    1602 
   Size:        520     480     602

  Fragments arranged by size: 

                 602     520     480
 
MspA1I CmG'CkG

Cuts at:      0      29     175     897     961    1602 
   Size:         29     146     722      64     641

  Fragments arranged by size: 

                 722     641     146      64      29
 
NaeI GCC'GGC

Cuts at:      0      89    1602 
   Size:         89    1513
 
NarI GG'CG_CC

Cuts at:      0      24    1602 
   Size:         24    1578
 
NcoI C'CATG_G

Cuts at:      0     413    1602 
   Size:        413    1189
 
NgoMIV G'CCGG_C

Cuts at:      0      87    1602 
   Size:         87    1515
 
NheI G'CTAG_C

Cuts at:      0    1419    1602 
   Size:       1419     183
 
NspI r_CATG'y

Cuts at:      0     697    1467    1602 
   Size:        697     770     135

  Fragments arranged by size: 

                 770     697     135
 
PciI A'CATG_T

Cuts at:      0    1463    1602 
   Size:       1463     139
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     102    1134    1602 
   Size:        102    1032     468

  Fragments arranged by size: 

                1032     468     102
 
PfoI T'CCnGG_A

Cuts at:      0     401     659    1133    1325    1602 
   Size:        401     258     474     192     277

  Fragments arranged by size: 

                 474     401     277     258     192
 
PmlI CAC'GTG

Cuts at:      0     234    1602 
   Size:        234    1368
 
PpuMI rG'GwC_Cy

Cuts at:      0    1131    1169    1602 
   Size:       1131      38     433

  Fragments arranged by size: 

                1131     433      38
 
PspOMI G'GGCC_C

Cuts at:      0     287     602    1602 
   Size:        287     315    1000

  Fragments arranged by size: 

                1000     315     287
 
PstI C_TGCA'G

Cuts at:      0    1040    1280    1524    1602 
   Size:       1040     240     244      78

  Fragments arranged by size: 

                1040     244     240      78
 
PvuII CAG'CTG

Cuts at:      0     897     961    1602 
   Size:        897      64     641

  Fragments arranged by size: 

                 897     641      64
 
SacII CC_GC'GG

Cuts at:      0      30    1602 
   Size:         30    1572
 
SalI G'TCGA_C

Cuts at:      0     465    1602 
   Size:        465    1137
 
SapI GCTCTTCn'nnn_

Cuts at:      0     401    1602 
   Size:        401    1201
 
ScaI AGT'ACT

Cuts at:      0     588     904    1602 
   Size:        588     316     698

  Fragments arranged by size: 

                 698     588     316
 
SexAI A'CCwGG_T

Cuts at:      0    1127    1602 
   Size:       1127     475
 
SfcI C'TryA_G

Cuts at:      0     780    1036    1276    1520    1602 
   Size:        780     256     240     244      82

  Fragments arranged by size: 

                 780     256     244     240      82
 
SfoI GGC'GCC

Cuts at:      0      25    1602 
   Size:         25    1577
 
SmaI CCC'GGG

Cuts at:      0     646    1602 
   Size:        646     956
 
SmlI C'TyrA_G

Cuts at:      0     380    1057    1091    1602 
   Size:        380     677      34     511

  Fragments arranged by size: 

                 677     511     380      34
 
SphI G_CATG'C

Cuts at:      0     697    1602 
   Size:        697     905
 
StyI C'CwwG_G

Cuts at:      0     212     413    1253    1316    1379    1565    1602 
   Size:        212     201     840      63      63     186      37

  Fragments arranged by size: 

                 840     212     201     186      63      63      37
 
TatI w'GTAC_w

Cuts at:      0     586     902    1602 
   Size:        586     316     700

  Fragments arranged by size: 

                 700     586     316
 
Tth111I GACn'n_nGTC

Cuts at:      0     205    1602 
   Size:        205    1397
 
XhoI C'TCGA_G

Cuts at:      0     380    1602 
   Size:        380    1222
 
XmaI C'CCGG_G

Cuts at:      0     644    1602 
   Size:        644     958
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AlfI    AlwNI
     ApaI    ApaLI     AvaI     BanI    BanII     BbeI    BbvCI     BcgI
     BclI    BglII     BlpI Bme1580I     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspHI    BsrFI    BssSI    BstXI    BstYI
     BtgI    BtgZI   DraIII     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NspI     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI
    PvuII    SacII     SalI     SapI     ScaI    SexAI     SfcI     SfoI
     SmaI     SmlI     SphI     StyI     TatI  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AleI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BciVI
    BfrBI     BglI    BmgBI     BmrI     BsaI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI  EcoICRI    EcoRI     FalI     FseI    FspAI
     HpaI     MfeI     MluI     MscI     NdeI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SanDI     SbfI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI
     XbaI     XcmI     XmnI     ZraI
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