Restriction map for pF1KB5224
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BsaBI       BsaHI       BsaI        BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     ClaI        CpoI        DpnII       
DraI        DraIII      EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MboI        
MfeI        MflI        MluI        MunI        NarI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccII         124  CGCG                 CG^CG
AccIII        386  TCCGGA               T^CCGGA
AcsI          663  GAATTT               [GA]^AATT[CT]
AocI          168  CCTCAGG              CC^T[ACGT]AGG
ApoI          663  GAATTT               [GA]^AATT[CT]
AspHI          63  GAGCAC               G[AT]GC[AT]^C
BanI          401  GGCACC               G^G[CT][GA]CC
BfaI          554  CTAG                 C^TAG
BglI           47  GCCGGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          701  GCTTAGC              GC^T[ACGT]AGC
BmyI           63  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      701  GCTTAGC              GC^T[ACGT]AGC
BsaAI         691  TACGTG               [CT]AC^GT[GA]
BseAI         386  TCCGGA               T^CCGGA
BsgI            3  GTGCAG               GTGCAG(16/14)
BsiEI         490  CGGTCG               CG[GA][CT]^CG
BsiHKAI        63  GAGCAC               G[AT]GC[AT]^C
BsmAI         557  GAGAC                GTCTC(1/5)
Bsp1286I       63  GAGCAC               G[AGT]GC[ACT]^C
BspEI         386  TCCGGA               T^CCGGA
BspHI          20  TCATGA               T^CATGA
BssSI         262  CACGAG               CACGAG(-5/-1)
BstAPI        534  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         124  CGCG                 CG^CG
Bsu36I        168  CCTCAGG              CC^T[ACGT]AGG
CelII         701  GCTTAGC              GC^T[ACGT]AGC
CfoI          125  GCGC                 GCG^C
DraII         512  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          142  GACTGTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          137  CCATGG               C^C[GA][CT]GG
Eco81I        168  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      512  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       137  CCATGG               C^C[AT][AT]GG
FauI          338  CCCGC                CCCGC(4/6)
HgaI          250  GACGC                GACGC(5/10)
HhaI          125  GCGC                 GCG^C
HinP1I        125  GCGC                 G^CGC
HincII        151  GTCAAC               GT[CT]^[GA]AC
HindII        151  GTCAAC               GT[CT]^[GA]AC
HphI          453  GGTGA                GGTGA(8/7)
MaeI          554  CTAG                 C^TAG
MaeII         692  ACGT                 A^CGT
MaeIII        620  GTCAC                ^GT[ACGT]AC
MroI          386  TCCGGA               T^CCGGA
MseI          671  TTAA                 T^TAA
MvnI          124  CGCG                 CG^CG
NaeI           52  GCCGGC               GCC^GGC
NcoI          137  CCATGG               C^CATGG
NgoMIV         52  GCCGGC               G^CCGGC
PflMI         132  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          111  GAGTC                GAGTC(4/5)
PpuMI         512  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         535  CAGCTG               CAG^CTG
RcaI           20  TCATGA               T^CATGA
SspI          391  AATATT               AAT^ATT
StuI          545  AGGCCT               AGG^CCT
StyI          137  CCATGG               C^C[AT][AT]GG
Tru9I         671  TTAA                 T^TAA
Tsp45I        620  GTCAC                ^GT[GC]AC
Tsp509I       664  AATT                 ^AATT
Van91I        132  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwNI         359  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              535  CAGCTGCTG           
BbsI          376  GAAGAC               GAAGAC(2/6)
              573  GAAGAC              
BcnI           48  CCGGG                CC^[GC]GG
              445  CCGGG               
BpmI           57  CTGGAG               CTGGAG(16/14)
              529  CTGGAG              
BpuAI         376  GAAGAC               GAAGAC(2/6)
              573  GAAGAC              
BsaWI         386  TCCGGA               [AT]^CCGG[AT]
              503  ACCGGA              
BsrFI          52  GCCGGC               [GA]^CCGG[CT]
              488  GCCGGT              
BstXI         521  CCACTGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              605  CCAAGAAGGTGG        
Cfr10I         52  GCCGGC               [GA]^CCGG[CT]
              488  GCCGGT              
EcoNI         405  CCTCGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              689  CCTACGTGAGG         
MspA1I          6  CAGCGG               C[AC]G^C[GT]G
              535  CAGCTG              
NciI           48  CCGGG                CC^[GC]GG
              445  CCGGG               
SfaNI         182  GCATC                GCATC(5/9)
              437  GCATC               
TfiI           75  GATTC                G^A[AT]TC
              361  GATTC               
XcmI          578  CCATGCTGGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              707  CCAGAAATGGGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AvaII       BalI        BbvI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        EaeI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
ItaI        MboII       MluNI       MnlI        MscI        
MslI        MspI        MvaI        MwoI        NlaIII      
NlaIV       PstI        Sau96I      ScrFI       SfcI        
TseI        TspRI       
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