Restriction map for pF1KB5224
 (Linear) (Six-base) MAPSORT of: KIBB5224.seq  Check: 8136  from: 1  to: 723



KIBB5224.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AleI CACnn'nnGTG

Cuts at:      0     658     691     723 
   Size:        658      33      32

  Fragments arranged by size: 

                 658      33      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     364     540     723 
   Size:        364     176     183

  Fragments arranged by size: 

                 364     183     176
 
ApoI r'AATT_y

Cuts at:      0     663     723 
   Size:        663      60
 
BanI G'GyrC_C

Cuts at:      0     401     723 
   Size:        401     322
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     383     580     723 
   Size:        383     197     143

  Fragments arranged by size: 

                 383     197     143
 
BglI GCCn_nnn'nGGC

Cuts at:      0      53     723 
   Size:         53     670
 
BlpI GC'TnA_GC

Cuts at:      0     702     723 
   Size:        702      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      97     265     279     723 
   Size:         97     168      14     444

  Fragments arranged by size: 

                 444     168      97      14
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     550     723 
   Size:         78     472     173

  Fragments arranged by size: 

                 472     173      78
 
Bpu10I CC'TnA_GC

Cuts at:      0     475     723 
   Size:        475     248
 
BsaAI yAC'GTr

Cuts at:      0     693     723 
   Size:        693      30
 
BsaWI w'CCGG_w

Cuts at:      0     386     503     723 
   Size:        386     117     220

  Fragments arranged by size: 

                 386     220     117
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75     511     711     723 
   Size:         75     436     200      12

  Fragments arranged by size: 

                 436     200      75      12
 
BseYI C'CCAG_C

Cuts at:      0      13     723 
   Size:         13     710
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     723 
   Size:         24     699
 
BsiEI CG_ry'CG

Cuts at:      0     493     723 
   Size:        493     230
 
BsiHKAI G_wGCw'C

Cuts at:      0      67     723 
   Size:         67     656
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     723 
   Size:         67     656
 
BspEI T'CCGG_A

Cuts at:      0     386     723 
   Size:        386     337
 
BspHI T'CATG_A

Cuts at:      0      20     723 
   Size:         20     703
 
BsrFI r'CCGG_y

Cuts at:      0      52     488     723 
   Size:         52     436     235

  Fragments arranged by size: 

                 436     235      52
 
BssSI C'ACGA_G

Cuts at:      0     262     723 
   Size:        262     461
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     540     723 
   Size:        540     183
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     528     612     723 
   Size:        528      84     111

  Fragments arranged by size: 

                 528     111      84
 
Bsu36I CC'TnA_GG

Cuts at:      0     169     723 
   Size:        169     554
 
BtgI C'CryG_G

Cuts at:      0     137     723 
   Size:        137     586
 
BtsI GCAGTG_nn'

Cuts at:      0     521     723 
   Size:        521     202
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     148     723 
   Size:        148     575
 
EaeI y'GGCC_r

Cuts at:      0     518     602     614     723 
   Size:        518      84      12     109

  Fragments arranged by size: 

                 518     109      84      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     550     723 
   Size:         78     472     173

  Fragments arranged by size: 

                 472     173      78
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     409     693     723 
   Size:        409     284      30

  Fragments arranged by size: 

                 409     284      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     513     723 
   Size:        513     210
 
HincII GTy'rAC

Cuts at:      0     153     723 
   Size:        153     570
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     473     723 
   Size:         94     379     250

  Fragments arranged by size: 

                 379     250      94
 
MscI TGG'CCA

Cuts at:      0     520     604     616     723 
   Size:        520      84      12     107

  Fragments arranged by size: 

                 520     107      84      12
 
MslI CAynn'nnrTG

Cuts at:      0     583     658     691     723 
   Size:        583      75      33      32

  Fragments arranged by size: 

                 583      75      33      32
 
MspA1I CmG'CkG

Cuts at:      0       8     537     723 
   Size:          8     529     186

  Fragments arranged by size: 

                 529     186       8
 
NaeI GCC'GGC

Cuts at:      0      54     723 
   Size:         54     669
 
NcoI C'CATG_G

Cuts at:      0     137     723 
   Size:        137     586
 
NgoMIV G'CCGG_C

Cuts at:      0      52     723 
   Size:         52     671
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     138     723 
   Size:        138     585
 
PpuMI rG'GwC_Cy

Cuts at:      0     513     723 
   Size:        513     210
 
PstI C_TGCA'G

Cuts at:      0     311     473     545     723 
   Size:        311     162      72     178

  Fragments arranged by size: 

                 311     178     162      72
 
PvuII CAG'CTG

Cuts at:      0     537     723 
   Size:        537     186
 
SfcI C'TryA_G

Cuts at:      0     307     469     541     723 
   Size:        307     162      72     182

  Fragments arranged by size: 

                 307     182     162      72
 
SspI AAT'ATT

Cuts at:      0     393     723 
   Size:        393     330
 
StuI AGG'CCT

Cuts at:      0     547     723 
   Size:        547     176
 
StyI C'CwwG_G

Cuts at:      0     137     723 
   Size:        137     586
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     613     645     723 
   Size:        613      32      78

  Fragments arranged by size: 

                 613      78      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     585     714     723 
   Size:        585     129       9

  Fragments arranged by size: 

                 585     129       9
 

 Enzymes that do cut:

     AleI    AlwNI     ApoI     BanI     BbsI     BglI     BlpI     BmrI
     BpmI   Bpu10I    BsaAI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BsrFI    BssSI   BstAPI    BstXI
   Bsu36I     BtgI     BtsI     DrdI     EaeI  Eco57MI    EcoNI EcoO109I
   HincII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
    PflMI    PpuMI     PstI    PvuII     SfcI     SspI     StuI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BsiWI
     BsmI    BsmBI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BstBI
   BstEII    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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