Restriction map for pF1KB5208
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       AccI        AccII       AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsiWI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspLU11I    BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PstI        PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     XbaI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        654  GGTACC               G^GTACC
AccIII        548  TCCGGA               T^CCGGA
AclI          826  AACGTT               AA^CGTT
Asp700I       960  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       654  GGTACC               G^GTACC
AspHI         268  GAGCTC               G[AT]GC[AT]^C
BanI          654  GGTACC               G^G[CT][GA]CC
BclI          482  TGATCA               T^GATCA
BsaWI         548  TCCGGA               [AT]^CCGG[AT]
BseAI         548  TCCGGA               T^CCGGA
BsgI          576  CTGCAC               GTGCAG(16/14)
BsiEI         414  CGACCG               CG[GA][CT]^CG
BsiHKAI       268  GAGCTC               G[AT]GC[AT]^C
BsmBI         837  CGTCTC               CGTCTC(1/5)
BspEI         548  TCCGGA               T^CCGGA
BspMI         303  GCAGGT               ACCTGC(4/8)
BsrDI         125  GCAATG               GCAATG(2/0)
BstAPI        386  GCATCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        759  GGTCACC              G^GT[ACGT]ACC
BstPI         759  GGTCACC              G^GT[ACGT]ACC
BstXI         776  CCATCGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         803  GCTGGC               GC[ACGT]^[ACGT]GC
DpnII         483  GATC                 ^GATC
DraII        1072  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoNI         731  CCTCTAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1072  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       759  GGTCACC              G^GT[ACGT]ACC
FbaI          482  TGATCA               T^GATCA
HincII         78  GTCAAC               GT[CT]^[GA]AC
HindII         78  GTCAAC               GT[CT]^[GA]AC
KpnI          654  GGTACC               GGTAC^C
MaeII         827  ACGT                 A^CGT
MboI          483  GATC                 ^GATC
MroI          548  TCCGGA               T^CCGGA
MseI          869  TTAA                 T^TAA
NdeII         483  GATC                 ^GATC
NspI          875  GCATGT               [GA]CATG^[CT]
PleI          503  GAGTC                GAGTC(4/5)
PpuMI        1072  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I      826  AACGTT               AA^CGTT
SacI          268  GAGCTC               GAGCT^C
Sau3AI        483  GATC                 ^GATC
TaqI          413  TCGA                 T^CGA
Tru9I         869  TTAA                 T^TAA
TthHB8I       413  TCGA                 T^CGA
XmnI          960  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BbsI          222  GAAGAC               GAAGAC(2/6)
              790  GTCTTC              
BbvI          572  GCAGC                GCAGC(8/12)
              575  GCTGC               
BpuAI         222  GAAGAC               GAAGAC(2/6)
              790  GTCTTC              
BsrBI         996  GAGCGG               CCGCTC(-3/-3)
             1015  GAGCGG              
DdeI          734  CTAAG                C^T[ACGT]AG
              749  CTGAG               
EarI          271  CTCTTC               CTCTTC(1/4)
              960  GAAGAG              
EcoT14I       488  CCATGG               C^C[AT][AT]GG
              842  CCATGG              
Ksp632I       271  CTCTTC               CTCTTC(1/4)
              960  GAAGAG              
MaeIII        760  GTCAC                ^GT[ACGT]AC
             1022  GTGAC               
MspA1I        573  CAGCTG               C[AC]G^C[GT]G
              912  CAGCTG              
NcoI          488  CCATGG               C^CATGG
              842  CCATGG              
PflMI          19  CCACTCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              581  CCATCAACTGG         
PvuII         573  CAGCTG               CAG^CTG
              912  CAGCTG              
SapI          960  GAAGAGC              GCTCTTC(1/4)
              270  GCTCTTC             
StyI          488  CCATGG               C^C[AT][AT]GG
              842  CCATGG              
TfiI          598  GAATC                G^A[AT]TC
              716  GAATC               
TseI          572  GCAGC                G^C[AT]GC
              575  GCTGC               
Tsp45I        760  GTCAC                ^GT[GC]AC
             1022  GTGAC               
TspRI         319  CACTG                CA[GC]TG[ACGT][ACGT]^
              559  CAGTG               
Van91I         19  CCACTCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              581  CCATCAACTGG         
XcmI          318  CCACTGGCCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              338  CCATTTGCCGGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BalI        
BanII       BcnI        BmyI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BspHI       BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DsaI        EaeI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MboII       MluNI       MnlI        
MscI        MslI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       RcaI        RsaI        
Sau96I      ScrFI       SfaNI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com