Restriction map for pF1KB5110
 (Circular) (Six-base) MAPSORT of: pF1KB5110.seq  Check: 9773  from: 1  to: 5027



pF1KB5110  5027 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:    344    1316    2065     344 
   Size:        972     749    3306

  Fragments arranged by size: 

                3306     972     749
 
Acc65I G'GTAC_C

Cuts at:   2043    2043 
   Size:       5027
 
AclI AA'CG_TT

Cuts at:   4867    4867 
   Size:       5027
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    961    1099    1431    2852    3284    3637    4299     961 
   Size:        138     332    1421     432     353     662    1689

  Fragments arranged by size: 

                1689    1421     662     432     353     332     138
 
AflIII A'CryG_T

Cuts at:    197    2379    4179    4460     197 
   Size:       2182    1800     281     764

  Fragments arranged by size: 

                2182    1800     764     281
 
AleI CACnn'nnGTG

Cuts at:   1564    1564 
   Size:       5027
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1582    1706    1582 
   Size:        124    4903
 
AlwNI CAG_nnn'CTG

Cuts at:    670     984    1296    2332    3770     670 
   Size:        314     312    1036    1438    1927

  Fragments arranged by size: 

                1927    1438    1036     314     312
 
ApaLI G'TGCA_C

Cuts at:   3865    3865 
   Size:       5027
 
ApoI r'AATT_y

Cuts at:    520    1873    2031    4380    4636     520 
   Size:       1353     158    2349     256     911

  Fragments arranged by size: 

                2349    1353     911     256     158
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5027
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5027
 
AvaI C'yCGr_G

Cuts at:    842    2047     842 
   Size:       1205    3822
 
BamHI G'GATC_C

Cuts at:   2052    2052 
   Size:       5027
 
BanI G'GyrC_C

Cuts at:   1951    2043    2457    2705    2740    1951 
   Size:         92     414     248      35    4238

  Fragments arranged by size: 

                4238     414     248      92      35
 
BanII G_rGCy'C

Cuts at:   2041    3071    2041 
   Size:       1030    3997
 
BbeI G_GCGC'C

Cuts at:   2461    2709    2461 
   Size:        248    4779
 
BbsI GAAGACnn'nnnn_

Cuts at:    679    1121     679 
   Size:        442    4585
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     997    1031    1920    1954      90 
   Size:         34     873      34     889      34    3163

  Fragments arranged by size: 

                3163     889     873      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2925    3981    5027    2925 
   Size:       1056    1046    2925

  Fragments arranged by size: 

                2925    1056    1046
 
BclI T'GATC_A

Cuts at:   1146    2547    1146 
   Size:       1401    3626
 
BglII A'GATC_T

Cuts at:   2542    4579    2542 
   Size:       2037    2990
 
BlpI GC'TnA_GC

Cuts at:   2139    2139 
   Size:       5027
 
Bme1580I G_kGCm'C

Cuts at:   1530    1954    2652    2745    3869    1530 
   Size:        424     698      93    1124    2688

  Fragments arranged by size: 

                2688    1124     698     424      93
 
BmgBI CAC'GTC

Cuts at:    363    1653     363 
   Size:       1290    3737
 
BmrI ACTGGGnnnn_n'

Cuts at:    865     968    1066    2239    2320    2655    4799     865 
   Size:        103      98    1173      81     335    2144    1093

  Fragments arranged by size: 

                2144    1173    1093     335     103      98      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3394    3394 
   Size:       5027
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1523    1523 
   Size:       5027
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    253    1125    2203    3082    3796    4094     253 
   Size:        872    1078     879     714     298    1186

  Fragments arranged by size: 

                1186    1078     879     872     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4731    4731 
   Size:       5027
 
BsaAI yAC'GTr

Cuts at:   1636    2380    1636 
   Size:        744    4283
 
BsaBI GATnn'nnATC

Cuts at:      6    2541    4578       6 
   Size:       2535    2037     455

  Fragments arranged by size: 

                2535    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2458    2706    3408    4904    2458 
   Size:        248     702    1496    2581

  Fragments arranged by size: 

                2581    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    184    2216    2737    3415    3826    3973    4247     184 
   Size:       2032     521     678     411     147     274     964

  Fragments arranged by size: 

                2032     964     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    496     742     766     496 
   Size:        246      24    4757

  Fragments arranged by size: 

                4757     246      24
 
BseYI C'CCAG_C

Cuts at:   1343    1483    2452    3210    3875    4283    1343 
   Size:        140     969     758     665     408    2087

  Fragments arranged by size: 

                2087     969     758     665     408     140
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1309    1588    1309 
   Size:        279    4748
 
BsiEI CG_ry'CG

Cuts at:    104    2569    2615    3845     104 
   Size:       2465      46    1230    1286

  Fragments arranged by size: 

                2465    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    152     504    2041    2819    3009    3869     152 
   Size:        352    1537     778     190     860    1310

  Fragments arranged by size: 

                1537    1310     860     778     352     190
 
Bsp1286I G_dGCh'C

Cuts at:    152     504    1530    1954    2041    2652    2745    2819    3009 
   Size:        352    1026     424      87     611      93      74     190

Cuts at:   3009    3071    3869     152 
   Size:         62     798    1310

  Fragments arranged by size: 

                1310    1026     798     611     424     352     190      93
                  87      74      62
 
BspHI T'CATG_A

Cuts at:   2012    3459    2012 
   Size:       1447    3580
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2077    2291    2593    2974    2077 
   Size:        214     302     381    4130

  Fragments arranged by size: 

                4130     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3319    3373    4857    5022      50 
   Size:       3269      54    1484     165      55

  Fragments arranged by size: 

                3269    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1154    1178    1457    1607    1622    1782    2939    1154 
   Size:         24     279     150      15     160    1157    3242

  Fragments arranged by size: 

                3242    1157     279     160     150      24      15
 
BsrFI r'CCGG_y

Cuts at:   1954    3025    3206    1954 
   Size:       1071     181    3775

  Fragments arranged by size: 

                3775    1071     181
 
BssSI C'ACGA_G

Cuts at:   3298    4006    3298 
   Size:        708    4319
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1180    1296    2404    1180 
   Size:        116    1108    3803

  Fragments arranged by size: 

                3803    1108     116
 
BstBI TT'CG_AA

Cuts at:   3388    3388 
   Size:       5027
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1215    1998    1215 
   Size:        783    4244
 
BstYI r'GATC_y

Cuts at:      1     323     398     494     981    2052    2519    2542    2877 
   Size:        322      75      96     487    1071     467      23     335

Cuts at:   2877    3123    3429    3441    3527    4579       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                1071    1052     487     467     449     335     322     306
                 246      96      86      75      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    917     917 
   Size:       5027
 
BtgI C'CryG_G

Cuts at:   1490    1562    1613    1784    1490 
   Size:         72      51     171    4733

  Fragments arranged by size: 

                4733     171      72      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2977    3158    3321    2977 
   Size:        181     163    4683

  Fragments arranged by size: 

                4683     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2270    2270 
   Size:       5027
 
DraI TTT'AAA

Cuts at:   2026    2026 
   Size:       5027
 
DraIII CAC_nnn'GTG

Cuts at:   1060    1060 
   Size:       5027
 
DrdI GACnn_nn'nnGTC

Cuts at:   1183    1795    2733    4077    1183 
   Size:        612     938    1344    2133

  Fragments arranged by size: 

                2133    1344     938     612
 
EaeI y'GGCC_r

Cuts at:   1554    1712    1956    2612    2786    3177    3204    4549    1554 
   Size:        158     244     656     174     391      27    1345    2032

  Fragments arranged by size: 

                2032    1345     656     391     244     174     158      27
            
 
EagI C'GGCC_G

Cuts at:   2612    2612 
   Size:       5027
 
EarI CTCTTCn'nnn_

Cuts at:   3050    3260    3050 
   Size:        210    4817
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3235    3977    4123    4837    3235 
   Size:        742     146     714    3425

  Fragments arranged by size: 

                3425     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2039    2039 
   Size:       5027
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    961    1099    1431    1523    2852    3284    3637    4299     961 
   Size:        138     332      92    1329     432     353     662    1689

  Fragments arranged by size: 

                1689    1329     662     432     353     332     138      92
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2014    2014 
   Size:       5027
 
EcoO109I rG'GnC_Cy

Cuts at:    697    1083    2166     697 
   Size:        386    1083    3558

  Fragments arranged by size: 

                3558    1083     386
 
EcoRI G'AATT_C

Cuts at:   2031    2031 
   Size:       5027
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1440    3046    1440 
   Size:       1606    3421
 
FspI TGC'GCA

Cuts at:   2808    2808 
   Size:       5027
 
HaeII r_GCGC'y

Cuts at:   1359    2461    2709    3939    4710    1359 
   Size:       1102     248    1230     771    1676

  Fragments arranged by size: 

                1676    1230    1102     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    486     518    1092    1108    1124    1131    1140    1163    2869 
   Size:         32     574      16      16       7       9      23    1706

Cuts at:   2869    2899    2901    2931     486 
   Size:         30       2      30    2582

  Fragments arranged by size: 

                2582    1706     574      32      30      30      23      16
                  16       9       7       2
 
HincII GTy'rAC

Cuts at:    195    2066     195 
   Size:       1871    3156
 
HpaI GTT'AAC

Cuts at:    195     195 
   Size:       5027
 
KasI G'GCGC_C

Cuts at:   2457    2705    2457 
   Size:        248    4779
 
KpnI G_GTAC'C

Cuts at:   2047    2047 
   Size:       5027
 
MluI A'CGCG_T

Cuts at:    197    4460     197 
   Size:       4263     764
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    426     815     863    1196    3786    3970     426 
   Size:        389      48     333    2590     184    1483

  Fragments arranged by size: 

                2590    1483     389     333     184      48
 
MscI TGG'CCA

Cuts at:   1714    2788    1714 
   Size:       1074    3953
 
MslI CAynn'nnrTG

Cuts at:    511    1564    1612    3016    3143     511 
   Size:       1053      48    1404     127    2395

  Fragments arranged by size: 

                2395    1404    1053     127      48
 
MspA1I CmG'CkG

Cuts at:   1500    1794    2138    2452    2812    3596    3841    1500 
   Size:        294     344     314     360     784     245    2686

  Fragments arranged by size: 

                2686     784     360     344     314     294     245
 
NaeI GCC'GGC

Cuts at:   3208    3208 
   Size:       5027
 
NarI GG'CG_CC

Cuts at:   2458    2706    2458 
   Size:        248    4779
 
NcoI C'CATG_G

Cuts at:   1562    1613    1562 
   Size:         51    4976
 
NdeI CA'TA_TG

Cuts at:    339     339 
   Size:       5027
 
NgoMIV G'CCGG_C

Cuts at:   3206    3206 
   Size:       5027
 
NspI r_CATG'y

Cuts at:   1068    1323    1683    2080    2580    4183    1068 
   Size:        255     360     397     500    1603    1912

  Fragments arranged by size: 

                1912    1603     500     397     360     255
 
PciI A'CATG_T

Cuts at:   4179    4179 
   Size:       5027
 
PflMI CCAn_nnn'nTGG

Cuts at:   1830    1830 
   Size:       5027
 
PfoI T'CCnGG_A

Cuts at:   1325    1325 
   Size:       5027
 
PmeI GTTT'AAAC

Cuts at:   2026    2026 
   Size:       5027
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3473    3505    3473 
   Size:         32    4995
 
PpuMI rG'GwC_Cy

Cuts at:    697     697 
   Size:       5027
 
PstI C_TGCA'G

Cuts at:    224     675     994    1176    1301    2074    2759     224 
   Size:        451     319     182     125     773     685    2492

  Fragments arranged by size: 

                2492     773     685     451     319     182     125
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5027
 
PvuII CAG'CTG

Cuts at:   1500    1794    2452    2812    1500 
   Size:        294     658     360    3715

  Fragments arranged by size: 

                3715     658     360     294
 
RsrII CG'GwC_CG

Cuts at:   3222    3222 
   Size:       5027
 
SacI G_AGCT'C

Cuts at:   2041    2041 
   Size:       5027
 
SalI G'TCGA_C

Cuts at:   2064    2064 
   Size:       5027
 
SapI GCTCTTCn'nnn_

Cuts at:   3050    3260    3050 
   Size:        210    4817
 
SbfI CC_TGCA'GG

Cuts at:   2074    2074 
   Size:       5027
 
ScaI AGT'ACT

Cuts at:    894    4544     894 
   Size:       3650    1377
 
SfcI C'TryA_G

Cuts at:     33     220     671     990    1172    1273    1297    2070    2755 
   Size:        187     451     319     182     101      24     773     685

Cuts at:   2755    3723    3914      33 
   Size:        968     191    1146

  Fragments arranged by size: 

                1146     968     773     685     451     319     191     187
                 182     101      24
 
SfoI GGC'GCC

Cuts at:   2459    2707    2459 
   Size:        248    4779
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5027
 
SmaI CCC'GGG

Cuts at:    844    2049     844 
   Size:       1205    3822
 
SmlI C'TyrA_G

Cuts at:    268    1104    2182    3097    3811    4073     268 
   Size:        836    1078     915     714     262    1222

  Fragments arranged by size: 

                1222    1078     915     836     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5027
 
SphI G_CATG'C

Cuts at:   1683    2080    2580    1683 
   Size:        397     500    4130

  Fragments arranged by size: 

                4130     500     397
 
StyI C'CwwG_G

Cuts at:    134     587    1046    1562    1613    1641    1814    1991    2161 
   Size:        453     459     516      51      28     173     177     170

Cuts at:   2161    2515     134 
   Size:        354    2646

  Fragments arranged by size: 

                2646     516     459     453     354     177     173     170
                  51      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    265     847    1048    2610    2743    3202    3411     265 
   Size:        582     201    1562     133     459     209    1881

  Fragments arranged by size: 

                1881    1562     582     459     209     201     133
 
TatI w'GTAC_w

Cuts at:    892    4542     892 
   Size:       3650    1377
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4544    4576    4544 
   Size:         32    4995
 
Tth111I GACn'n_nGTC

Cuts at:    960    2824     960 
   Size:       1864    3163
 
XbaI T'CTAG_A

Cuts at:   2058    2058 
   Size:       5027
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1053    1944    1998    2522    1053 
   Size:        891      54     524    3558

  Fragments arranged by size: 

                3558     891     524      54
 
XmaI C'CCGG_G

Cuts at:    842    2047     842 
   Size:       1205    3822
 
XmnI GAAnn'nnTTC

Cuts at:   1444    1444 
   Size:       5027
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI     BmtI   Bpu10I
    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BsrGI   BssHII   BstEII
  BstZ17I     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII     MfeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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