Restriction map for pF1KB5108
 (Linear) (Six-base) MAPSORT of: KIBB5108.seq  Check: 1267  from: 1  to: 2385



KIBB5108.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

Acc65I G'GTAC_C

Cuts at:      0      20    2385 
   Size:         20    2365
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     941    1684    1709    1745    1771    1838    2385 
   Size:        941     743      25      36      26      67     547

  Fragments arranged by size: 

                 941     743     547      67      36      26      25
 
AgeI A'CCGG_T

Cuts at:      0     259    2385 
   Size:        259    2126
 
AleI CACnn'nnGTG

Cuts at:      0    1871    2385 
   Size:       1871     514
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1489    1510    2385 
   Size:       1489      21     875

  Fragments arranged by size: 

                1489     875      21
 
ApaI G_GGCC'C

Cuts at:      0     141    2385 
   Size:        141    2244
 
ApoI r'AATT_y

Cuts at:      0     364     610    1198    1345    2376    2385 
   Size:        364     246     588     147    1031       9

  Fragments arranged by size: 

                1031     588     364     246     147       9
 
AvaI C'yCGr_G

Cuts at:      0     113    2385 
   Size:        113    2272
 
BanI G'GyrC_C

Cuts at:      0      20    2385 
   Size:         20    2365
 
BanII G_rGCy'C

Cuts at:      0     141    1441    1911    2385 
   Size:        141    1300     470     474

  Fragments arranged by size: 

                1300     474     470     141
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     578    2385 
   Size:        578    1807
 
BfrBI ATG'CAT

Cuts at:      0     215     284    2385 
   Size:        215      69    2101

  Fragments arranged by size: 

                2101     215      69
 
BglII A'GATC_T

Cuts at:      0     473     757    2385 
   Size:        473     284    1628

  Fragments arranged by size: 

                1628     473     284
 
BlpI GC'TnA_GC

Cuts at:      0      28    1001    2385 
   Size:         28     973    1384

  Fragments arranged by size: 

                1384     973      28
 
Bme1580I G_kGCm'C

Cuts at:      0     141    2385 
   Size:        141    2244
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1865    2385 
   Size:       1865     520
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210    1747    2385 
   Size:        210    1537     638

  Fragments arranged by size: 

                1537     638     210
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     110    2385 
   Size:        110    2275
 
BsaBI GATnn'nnATC

Cuts at:      0    1316    2385 
   Size:       1316    1069
 
BsaWI w'CCGG_w

Cuts at:      0     259    2385 
   Size:        259    2126
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      86     116    1092    1122    2385 
   Size:         86      30     976      30    1263

  Fragments arranged by size: 

                1263     976      86      30      30
 
BseYI C'CCAG_C

Cuts at:      0    1393    2385 
   Size:       1393     992
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1667    1820    2385 
   Size:       1667     153     565

  Fragments arranged by size: 

                1667     565     153
 
BsiHKAI G_wGCw'C

Cuts at:      0     792     839    2385 
   Size:        792      47    1546

  Fragments arranged by size: 

                1546     792      47
 
BsiWI C'GTAC_G

Cuts at:      0      60    2385 
   Size:         60    2325
 
BsmI GAATG_Cn'

Cuts at:      0    2362    2385 
   Size:       2362      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     141     792     839    1441    1911    2385 
   Size:        141     651      47     602     470     474

  Fragments arranged by size: 

                 651     602     474     470     141      47
 
BspHI T'CATG_A

Cuts at:      0    2024    2385 
   Size:       2024     361
 
BsrBI CCG'CTC

Cuts at:      0       8    2385 
   Size:          8    2377
 
BsrDI GCAATG_nn'

Cuts at:      0    1899    2096    2385 
   Size:       1899     197     289

  Fragments arranged by size: 

                1899     289     197
 
BsrFI r'CCGG_y

Cuts at:      0      23     259    2385 
   Size:         23     236    2126

  Fragments arranged by size: 

                2126     236      23
 
BsrGI T'GTAC_A

Cuts at:      0    2277    2385 
   Size:       2277     108
 
BssSI C'ACGA_G

Cuts at:      0      49    2385 
   Size:         49    2336
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     631    2385 
   Size:        631    1754
 
BstYI r'GATC_y

Cuts at:      0     473     757    1404    1447    1473    2385 
   Size:        473     284     647      43      26     912

  Fragments arranged by size: 

                 912     647     473     284      43      26
 
BtgI C'CryG_G

Cuts at:      0    1529    2385 
   Size:       1529     856
 
BtsI GCAGTG_nn'

Cuts at:      0     398     464    2385 
   Size:        398      66    1921

  Fragments arranged by size: 

                1921     398      66
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1215    1250    2385 
   Size:       1215      35    1135

  Fragments arranged by size: 

                1215    1135      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1058    2385 
   Size:       1058    1327
 
EaeI y'GGCC_r

Cuts at:      0     186    1532    2004    2385 
   Size:        186    1346     472     381

  Fragments arranged by size: 

                1346     472     381     186
 
EarI CTCTTCn'nnn_

Cuts at:      0    1446    1685    2385 
   Size:       1446     239     700

  Fragments arranged by size: 

                1446     700     239
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      77    2385 
   Size:         77    2308
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     941    1684    1709    1745    1771    1838    2385 
   Size:        941     743      25      36      26      67     547

  Fragments arranged by size: 

                 941     743     547      67      36      26      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1481    2385 
   Size:       1481     904
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     138    2385 
   Size:         66      72    2247

  Fragments arranged by size: 

                2247      72      66
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     742     774    1008    1040    1725    1757    2006    2038 
   Size:        742      32     234      32     685      32     249      32

Cuts at:   2038    2385 
   Size:        347

  Fragments arranged by size: 

                 742     685     347     249     234      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     500    1283    1703    2385 
   Size:        500     783     420     682

  Fragments arranged by size: 

                 783     682     500     420
 
HpaI GTT'AAC

Cuts at:      0    1283    1703    2385 
   Size:       1283     420     682

  Fragments arranged by size: 

                1283     682     420
 
KpnI G_GTAC'C

Cuts at:      0      24    2385 
   Size:         24    2361
 
MfeI C'AATT_G

Cuts at:      0    1217    1992    2385 
   Size:       1217     775     393

  Fragments arranged by size: 

                1217     775     393
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     825    2385 
   Size:        825    1560
 
MscI TGG'CCA

Cuts at:      0     188    2006    2385 
   Size:        188    1818     379

  Fragments arranged by size: 

                1818     379     188
 
MslI CAynn'nnrTG

Cuts at:      0    1028    1871    2385 
   Size:       1028     843     514

  Fragments arranged by size: 

                1028     843     514
 
MspA1I CmG'CkG

Cuts at:      0     454    1576    1968    2385 
   Size:        454    1122     392     417

  Fragments arranged by size: 

                1122     454     417     392
 
NcoI C'CATG_G

Cuts at:      0    1529    2385 
   Size:       1529     856
 
NsiI A_TGCA'T

Cuts at:      0     217     286    2385 
   Size:        217      69    2099

  Fragments arranged by size: 

                2099     217      69
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      54    2385 
   Size:         54    2331
 
PspOMI G'GGCC_C

Cuts at:      0     137    2385 
   Size:        137    2248
 
PstI C_TGCA'G

Cuts at:      0     459    2385 
   Size:        459    1926
 
PvuII CAG'CTG

Cuts at:      0     454    1576    1968    2385 
   Size:        454    1122     392     417

  Fragments arranged by size: 

                1122     454     417     392
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1446    2385 
   Size:       1446     939
 
SexAI A'CCwGG_T

Cuts at:      0      16    1035    1503    2385 
   Size:         16    1019     468     882

  Fragments arranged by size: 

                1019     882     468      16
 
SfcI C'TryA_G

Cuts at:      0     455    2361    2385 
   Size:        455    1906      24

  Fragments arranged by size: 

                1906     455      24
 
SgrAI Cr'CCGG_yG

Cuts at:      0     259    2385 
   Size:        259    2126
 
SmlI C'TyrA_G

Cuts at:      0     225    1726    2385 
   Size:        225    1501     659

  Fragments arranged by size: 

                1501     659     225
 
SspI AAT'ATT

Cuts at:      0    1596    1652    2385 
   Size:       1596      56     733

  Fragments arranged by size: 

                1596     733      56
 
StyI C'CwwG_G

Cuts at:      0    1276    1529    2385 
   Size:       1276     253     856

  Fragments arranged by size: 

                1276     856     253
 
TatI w'GTAC_w

Cuts at:      0    2205    2277    2385 
   Size:       2205      72     108

  Fragments arranged by size: 

                2205     108      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      86     118    1092    1124    1458    1490    2385 
   Size:         86      32     974      32     334      32     895

  Fragments arranged by size: 

                 974     895     334      86      32      32      32
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AleI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BfrBI    BglII     BlpI Bme1580I     BmrI
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstYI     BtgI     BtsI    CspCI     DrdI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     HpaI
     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI
    PflMI   PspOMI     PstI    PvuII     SapI    SexAI     SfcI    SgrAI
     SmlI     SspI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaHI    BseRI    BsiEI
    BsmBI    BspEI    BspMI   BssHII    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI     DraI   DraIII     EagI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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