Restriction map for pF1KB5104
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoT22I     FauI        FseI        
FspI        HaeII       HhaI        HinP1I      KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII       1015  TCCGGA               T^CCGGA
AcyI         1625  GGCGTC               G[GA]^CG[CT]C
AflIII        664  ACACGT               A^C[GA][CT]GT
AseI         1201  ATTAAT               AT^TAAT
AsnI         1201  ATTAAT               AT^TAAT
AspHI          65  GAGCTC               G[AT]GC[AT]^C
BbsI         1081  GAAGAC               GAAGAC(2/6)
BbvCI         415  GCTGAGG              CCTCAGC(-5/-2)
BclI          407  TGATCA               T^GATCA
BpuAI        1081  GAAGAC               GAAGAC(2/6)
BsaHI        1625  GGCGTC               G[GA]^CG[CT]C
BsaJI         935  CCATGG               C^C[ACGT][ACGT]GG
BsaWI        1015  TCCGGA               [AT]^CCGG[AT]
BseAI        1015  TCCGGA               T^CCGGA
BsiHKAI        65  GAGCTC               G[AT]GC[AT]^C
BsiYI         584  CCATATTTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          584  CCATATTTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI        1539  GAGAC                GTCTC(1/5)
BspEI        1015  TCCGGA               T^CCGGA
DraI          438  TTTAAA               TTT^AAA
DraII         592  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          935  CCATGG               C^C[GA][CT]GG
EcoO109I      592  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         571  GATATC               GAT^ATC
EcoT14I       935  CCATGG               C^C[AT][AT]GG
FbaI          407  TGATCA               T^GATCA
Hin1I        1625  GGCGTC               G[GA]^CG[CT]C
HincII       1251  GTTAAC               GT[CT]^[GA]AC
HindII       1251  GTTAAC               GT[CT]^[GA]AC
HindIII      1235  AAGCTT               A^AGCTT
HpaI         1251  GTTAAC               GTT^AAC
HphI         1303  TCACC                GGTGA(8/7)
MaeII         666  ACGT                 A^CGT
MroI         1015  TCCGGA               T^CCGGA
NcoI          935  CCATGG               C^CATGG
NdeI          133  CATATG               CA^TATG
NspI          207  ACATGC               [GA]CATG^[CT]
PpuMI         592  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1201  ATTAAT               AT^TAAT
SacI           65  GAGCTC               GAGCT^C
SfcI         1174  CTACAG               C^T[GA][CT]AG
SpeI          985  ACTAGT               A^CTAGT
StuI          756  AGGCCT               AGG^CCT
StyI          935  CCATGG               C^C[AT][AT]GG
XcmI         1343  CCATAAACCAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI           72  GTAC                 GT^AC
             1286  GTAC                
AlwNI         289  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              292  CAGCTGCTG           
BanI           99  GGCACC               G^G[CT][GA]CC
             1356  GGTGCC              
BanII          65  GAGCTC               G[GA]GC[CT]^C
              991  GAGCCC              
BpmI         1585  CTCCAG               CTGGAG(16/14)
             1307  CTGGAG              
BsmFI         215  GGGAC                GGGAC(10/14)
              678  GTCCC               
BsmI          347  GAATGC               GAATGC(1/-1)
              447  GAATGC              
BssKI         942  CCTGG                ^CC[ACGT]GG
             1306  CCTGG               
BstAPI        291  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              730  GCAGAATGTGC         
BstNI         942  CCTGG                CC^[AT]GG
             1306  CCTGG               
BstYI         360  AGATCC               [GA]^GATC[CT]
              720  GGATCT              
Cac8I        1038  GCCAGC               GC[ACGT]^[ACGT]GC
             1338  GCAAGC              
EarI          334  GAAGAG               CTCTTC(1/4)
             1576  GAAGAG              
EcoRI         421  GAATTC               G^AATTC
              655  GAATTC              
EcoRII        942  CCTGG                ^CC[AT]GG
             1306  CCTGG               
HapII         522  CCGG                 C^CGG
             1016  CCGG                
HgaI           45  GCGTC                GACGC(5/10)
             1626  GCGTC               
HpaII         522  CCGG                 C^CGG
             1016  CCGG                
Ksp632I       334  GAAGAG               CTCTTC(1/4)
             1576  GAAGAG              
MflI          360  AGATCC               [GA]^GATC[CT]
              720  GGATCT              
MspI          522  CCGG                 C^CGG
             1016  CCGG                
MvaI          942  CCTGG                CC^[AT]GG
             1306  CCTGG               
RsaI           72  GTAC                 GT^AC
             1286  GTAC                
ScrFI         942  CCTGG                CC^[ACGT]GG
             1306  CCTGG               
SmlI         1126  CTCAAG               C^T[CT][GA]AG
             1266  CTCAAG              
TfiI         1496  GATTC                G^A[AT]TC
             1533  GATTC               
XhoII         360  AGATCC               [GA]^GATC[CT]
              720  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        Asp700I     
AvaII       BbvI        BfaI        BmyI        Bsp1286I    
BsrI        BstF5I      BstXI       Cfr13I      DdeI        
DpnII       Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MwoI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
Sau3AI      Sau96I      SfaNI       Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XmnI        
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