Restriction map for pF1KB5091
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AcyI        AflII       
AflIII      AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MfeI        MluI        MluNI       MscI        MspA1I      
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PvuI        PvuII       RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII       1756  TCCGGA               T^CCGGA
Alw44I        220  GTGCAC               G^TGCAC
ApaLI         220  GTGCAC               G^TGCAC
AvaII          30  GGTCC                G^G[AT]CC
BanI          840  GGTGCC               G^G[CT][GA]CC
BcnI          134  CCCGG                CC^[GC]GG
BlpI           39  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       39  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1768  GATATCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1756  TCCGGA               T^CCGGA
BseRI         785  CTCCTC               GAGGAG(10/8)
BsmI          553  GCATTC               GAATGC(1/-1)
BspDI        1450  ATCGAT               AT^CGAT
BspEI        1756  TCCGGA               T^CCGGA
BspHI        1149  TCATGA               T^CATGA
BsrBI        1752  CCGCTC               CCGCTC(-3/-3)
BstAPI        553  GCATTCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         281  TTCGAA               TT^CGAA
BstXI         620  CCAACGGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1707  AGATCC               [GA]^GATC[CT]
CelII          39  GCTCAGC              GC^T[ACGT]AGC
ClaI         1450  ATCGAT               AT^CGAT
EaeI         1495  CGGCCA               [CT]^GGCC[GA]
EcoNI         693  CCTTTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1768  GATATC               GAT^ATC
HaeII          36  GGCGCT               [GA]GCGC^[CT]
HincII        306  GTTGAC               GT[CT]^[GA]AC
HindII        306  GTTGAC               GT[CT]^[GA]AC
MamI         1768  GATATCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1707  AGATCC               [GA]^GATC[CT]
MroI         1756  TCCGGA               T^CCGGA
MslI         1009  CACTTGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          134  CCCGG                CC^[GC]GG
NlaIV         840  GGTGCC               GG[ACGT]^[ACGT]CC
NspV          281  TTCGAA               TT^CGAA
PleI          438  GACTC                GAGTC(4/5)
PstI         1421  CTGCAG               CTGCA^G
RcaI         1149  TCATGA               T^CATGA
SacI         1714  GAGCTC               GAGCT^C
ScaI         1782  AGTACT               AGT^ACT
SfcI         1421  CTGCAG               C^T[GA][CT]AG
SfuI          281  TTCGAA               TT^CGAA
XhoII        1707  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccII          10  CGCG                 CG^CG
               27  CGCG                
AclI          256  AACGTT               AA^CGTT
             1796  AACGTT              
AlwI          526  GATCC                GGATC(4/5)
             1708  GATCC               
BciVI         741  GTATCC               GTATCC(6/5)
             1467  GTATCC              
BsaWI         168  ACCGGA               [AT]^CCGG[AT]
             1756  TCCGGA              
BsmAI         973  GAGAC                GTCTC(1/5)
             1653  GTCTC               
BsmFI         793  GTCCC                GGGAC(10/14)
             1682  GTCCC               
BstUI          10  CGCG                 CG^CG
               27  CGCG                
DsaI          633  CCATGG               C^C[GA][CT]GG
             1520  CCATGG              
MaeIII       1445  GTGAC                ^GT[ACGT]AC
             1729  GTCAC               
MvnI           10  CGCG                 CG^CG
               27  CGCG                
NcoI          633  CCATGG               C^CATGG
             1520  CCATGG              
PflMI         633  CCATGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              863  CCACAATCTGG         
Psp1406I      256  AACGTT               AA^CGTT
             1796  AACGTT              
SmlI          788  CTCAAG               C^T[CT][GA]AG
             1011  CTTGAG              
Tsp45I       1445  GTGAC                ^GT[GC]AC
             1729  GTCAC               
Van91I        633  CCATGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              863  CCACAATCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       BanII       BbvI        BfaI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MboI        
MboII       MnlI        MseI        MspI        MvaI        
MwoI        NdeII       NlaIII      RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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