Restriction map for pF1KB5091
 (Circular) (Six-base) MAPSORT of: pF1KB5091.seq  Check: 7707  from: 1  to: 4925



pF1KB5091  4925 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:   1963    1963 
   Size:       4925
 
Acc65I G'GTAC_C

Cuts at:   1941    1941 
   Size:       4925
 
AclI AA'CG_TT

Cuts at:    365    1905    4765     365 
   Size:       1540    2860     525

  Fragments arranged by size: 

                2860    1540     525
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1501    2750    3182    3535    4197    1501 
   Size:       1249     432     353     662    2229

  Fragments arranged by size: 

                2229    1249     662     432     353
 
AflIII A'CryG_T

Cuts at:   2277    4077    4358    2277 
   Size:       1800     281    2844

  Fragments arranged by size: 

                2844    1800     281
 
AleI CACnn'nnGTG

Cuts at:   1121    1121 
   Size:       4925
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    183     183 
   Size:       4925
 
AlwNI CAG_nnn'CTG

Cuts at:   2230    3668    2230 
   Size:       1438    3487
 
ApaLI G'TGCA_C

Cuts at:    328    3763     328 
   Size:       3435    1490
 
ApoI r'AATT_y

Cuts at:    280    1144    1204    1253    1929    4278    4534     280 
   Size:        864      60      49     676    2349     256     671

  Fragments arranged by size: 

                2349     864     676     671     256      60      49
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4925
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4925
 
AvaI C'yCGr_G

Cuts at:   1945    1945 
   Size:       4925
 
BamHI G'GATC_C

Cuts at:   1950    1950 
   Size:       4925
 
BanI G'GyrC_C

Cuts at:    948    1941    2355    2603    2638     948 
   Size:        993     414     248      35    3235

  Fragments arranged by size: 

                3235     993     414     248      35
 
BanII G_rGCy'C

Cuts at:    519     646    1826    1939    2969     519 
   Size:        127    1180     113    1030    2475

  Fragments arranged by size: 

                2475    1180    1030     127     113
 
BbeI G_GCGC'C

Cuts at:   2359    2607    2359 
   Size:        248    4677
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4891
 
BciVI GTATCCnnnnn_n'

Cuts at:    860    1586    2823    3879    4925     860 
   Size:        726    1237    1056    1046     860

  Fragments arranged by size: 

                1237    1056    1046     860     726
 
BclI T'GATC_A

Cuts at:   2445    2445 
   Size:       4925
 
BglII A'GATC_T

Cuts at:   2440    4477    2440 
   Size:       2037    2888
 
BlpI GC'TnA_GC

Cuts at:    148    2037     148 
   Size:       1889    3036
 
Bme1580I G_kGCm'C

Cuts at:    332    2550    2643    3767     332 
   Size:       2218      93    1124    1490

  Fragments arranged by size: 

                2218    1490    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1320    2137    2218    2553    4697    1320 
   Size:        817      81     335    2144    1548

  Fragments arranged by size: 

                2144    1548     817     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3292    3292 
   Size:       4925
 
Bpu10I CC'TnA_GC

Cuts at:    905     905 
   Size:       4925
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    881    1140    2101    2980    3694    3992     881 
   Size:        259     961     879     714     298    1814

  Fragments arranged by size: 

                1814     961     879     714     298     259
 
BsaI GGTCTCn'nnnn_

Cuts at:   4629    4629 
   Size:       4925
 
BsaAI yAC'GTr

Cuts at:   2278    2278 
   Size:       4925
 
BsaBI GATnn'nnATC

Cuts at:      6    1880    2439    4476       6 
   Size:       1874     559    2037     455

  Fragments arranged by size: 

                2037    1874     559     455
 
BsaHI Gr'CG_yC

Cuts at:   2356    2604    3306    4802    2356 
   Size:        248     702    1496    2479

  Fragments arranged by size: 

                2479    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    276    1864    2114    2635    3313    3724    3871    4145     276 
   Size:       1588     250     521     678     411     147     274    1056

  Fragments arranged by size: 

                1588    1056     678     521     411     274     250     147
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    884     884 
   Size:       4925
 
BseYI C'CCAG_C

Cuts at:    479     671     735    2350    3108    3773    4181     479 
   Size:        192      64    1615     758     665     408    1223

  Fragments arranged by size: 

                1615    1223     758     665     408     192      64
 
BsiEI CG_ry'CG

Cuts at:    104    2467    2513    3743     104 
   Size:       2363      46    1230    1286

  Fragments arranged by size: 

                2363    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    332    1647    1826    1939    2717    2907    3767     332 
   Size:       1315     179     113     778     190     860    1490

  Fragments arranged by size: 

                1490    1315     860     778     190     179     113
 
BsmI GAATG_Cn'

Cuts at:    661     661 
   Size:       4925
 
Bsp1286I G_dGCh'C

Cuts at:    332     519     646    1647    1826    1939    2550    2643    2717 
   Size:        187     127    1001     179     113     611      93      74

Cuts at:   2717    2907    2969    3767     332 
   Size:        190      62     798    1490

  Fragments arranged by size: 

                1490    1001     798     611     190     187     179     127
                 113      93      74      62
 
BspEI T'CCGG_A

Cuts at:   1864    1864 
   Size:       4925
 
BspHI T'CATG_A

Cuts at:   1257    3357    1257 
   Size:       2100    2825
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1975    2189    2491    2872    1975 
   Size:        214     302     381    4028

  Fragments arranged by size: 

                4028     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1862    3217    3271    4755    4920      50 
   Size:       1812    1355      54    1484     165      55

  Fragments arranged by size: 

                1812    1484    1355     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2837    2837 
   Size:       4925
 
BsrFI r'CCGG_y

Cuts at:   2923    3104    2923 
   Size:        181    4744
 
BssSI C'ACGA_G

Cuts at:   3196    3904    3196 
   Size:        708    4217
 
BstAPI GCAn_nnn'nTGC

Cuts at:    667    2302     667 
   Size:       1635    3290
 
BstBI TT'CG_AA

Cuts at:    390    3286     390 
   Size:       2896    2029
 
BstXI CCAn_nnnn'nTGG

Cuts at:    735     735 
   Size:       4925
 
BstYI r'GATC_y

Cuts at:      1    1815    1950    2417    2440    2775    3021    3327    3339 
   Size:       1814     135     467      23     335     246     306      12

Cuts at:   3339    3425    4477       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1814    1052     467     449     335     306     246     135
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    741    1628     741 
   Size:        887    4038
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1019    2875    3056    3219    1019 
   Size:       1856     181     163    2725

  Fragments arranged by size: 

                2725    1856     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1745    2168    1745 
   Size:        423    4502
 
ClaI AT'CG_AT

Cuts at:   1559    1559 
   Size:       4925
 
DraI TTT'AAA

Cuts at:   1924    1924 
   Size:       4925
 
DrdI GACnn_nn'nnGTC

Cuts at:   2631    3975    2631 
   Size:       1344    3581
 
EaeI y'GGCC_r

Cuts at:   1603    2510    2684    3075    3102    4447    1603 
   Size:        907     174     391      27    1345    2081

  Fragments arranged by size: 

                2081    1345     907     391     174      27
 
EagI C'GGCC_G

Cuts at:   2510    2510 
   Size:       4925
 
EarI CTCTTCn'nnn_

Cuts at:   2948    3158    2948 
   Size:        210    4715
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3133    3875    4021    4735    3133 
   Size:        742     146     714    3323

  Fragments arranged by size: 

                3323     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1824    1937    1824 
   Size:        113    4812
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1501    2750    3182    3535    4197    1501 
   Size:       1249     432     353     662    2229

  Fragments arranged by size: 

                2229    1249     662     432     353
 
EcoNI CCTnn'n_nnAGG

Cuts at:    805     805 
   Size:       4925
 
EcoO109I rG'GnC_Cy

Cuts at:   2064    2064 
   Size:       4925
 
EcoRI G'AATT_C

Cuts at:   1929    1929 
   Size:       4925
 
EcoRV GAT'ATC

Cuts at:   1878    1878 
   Size:       4925
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2944    2944 
   Size:       4925
 
FspI TGC'GCA

Cuts at:   2706    2706 
   Size:       4925
 
HaeII r_GCGC'y

Cuts at:    148    2359    2607    3837    4608     148 
   Size:       2211     248    1230     771     465

  Fragments arranged by size: 

                2211    1230     771     465     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    426     458     837     869     876     908    1113    1145    2767 
   Size:         32     379      32       7      32     205      32    1622

Cuts at:   2767    2797    2799    2829     426 
   Size:         30       2      30    2522

  Fragments arranged by size: 

                2522    1622     379     205      32      32      32      32
                  30      30       7       2
 
HincII GTy'rAC

Cuts at:    416    1964     416 
   Size:       1548    3377
 
KasI G'GCGC_C

Cuts at:   2355    2603    2355 
   Size:        248    4677
 
KpnI G_GTAC'C

Cuts at:   1945    1945 
   Size:       4925
 
MluI A'CGCG_T

Cuts at:   4358    4358 
   Size:       4925
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1517    1620    3684    3868    1517 
   Size:        103    2064     184    2574

  Fragments arranged by size: 

                2574    2064     184     103
 
MscI TGG'CCA

Cuts at:   2686    2686 
   Size:       4925
 
MslI CAynn'nnrTG

Cuts at:   1121    2914    3041    1121 
   Size:       1793     127    3005

  Fragments arranged by size: 

                3005    1793     127
 
MspA1I CmG'CkG

Cuts at:   2036    2350    2710    3494    3739    2036 
   Size:        314     360     784     245    3222

  Fragments arranged by size: 

                3222     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   3106    3106 
   Size:       4925
 
NarI GG'CG_CC

Cuts at:   2356    2604    2356 
   Size:        248    4677
 
NcoI C'CATG_G

Cuts at:    741    1628     741 
   Size:        887    4038
 
NgoMIV G'CCGG_C

Cuts at:   3104    3104 
   Size:       4925
 
NspI r_CATG'y

Cuts at:   1978    2478    4081    1978 
   Size:        500    1603    2822

  Fragments arranged by size: 

                2822    1603     500
 
PasI CC'CwG_GG

Cuts at:    639     639 
   Size:       4925
 
PciI A'CATG_T

Cuts at:   4077    4077 
   Size:       4925
 
PflMI CCAn_nnn'nTGG

Cuts at:    747     977     747 
   Size:        230    4695
 
PmeI GTTT'AAAC

Cuts at:   1924    1924 
   Size:       4925
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1033    1065    3371    3403    1033 
   Size:         32    2306      32    2555

  Fragments arranged by size: 

                2555    2306      32      32
 
PstI C_TGCA'G

Cuts at:   1533    1972    2657    1533 
   Size:        439     685    3801

  Fragments arranged by size: 

                3801     685     439
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4925
 
PvuII CAG'CTG

Cuts at:   2350    2710    2350 
   Size:        360    4565
 
RsrII CG'GwC_CG

Cuts at:   3120    3120 
   Size:       4925
 
SacI G_AGCT'C

Cuts at:   1826    1939    1826 
   Size:        113    4812
 
SalI G'TCGA_C

Cuts at:   1962    1962 
   Size:       4925
 
SapI GCTCTTCn'nnn_

Cuts at:   2948    3158    2948 
   Size:        210    4715
 
SbfI CC_TGCA'GG

Cuts at:   1972    1972 
   Size:       4925
 
ScaI AGT'ACT

Cuts at:   1892    4442    1892 
   Size:       2550    2375
 
SfcI C'TryA_G

Cuts at:     33    1529    1968    2653    3621    3812      33 
   Size:       1496     439     685     968     191    1146

  Fragments arranged by size: 

                1496    1146     968     685     439     191
 
SfoI GGC'GCC

Cuts at:   2357    2605    2357 
   Size:        248    4677
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4925
 
SmaI CCC'GGG

Cuts at:   1947    1947 
   Size:       4925
 
SmlI C'TyrA_G

Cuts at:    896    1119    2080    2995    3709    3971     896 
   Size:        223     961     915     714     262    1850

  Fragments arranged by size: 

                1850     961     915     714     262     223
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4925
 
SphI G_CATG'C

Cuts at:   1978    2478    1978 
   Size:        500    4425
 
StyI C'CwwG_G

Cuts at:    551     741    1628    2059    2413     551 
   Size:        190     887     431     354    3063

  Fragments arranged by size: 

                3063     887     431     354     190
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    194     544    2508    2641    3100    3309     194 
   Size:        350    1964     133     459     209    1810

  Fragments arranged by size: 

                1964    1810     459     350     209     133
 
TatI w'GTAC_w

Cuts at:   1395    1890    4440    1395 
   Size:        495    2550    1880

  Fragments arranged by size: 

                2550    1880     495
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4442    4474    4442 
   Size:         32    4893
 
Tth111I GACn'n_nGTC

Cuts at:   2722    2722 
   Size:       4925
 
XbaI T'CTAG_A

Cuts at:   1956    1956 
   Size:       4925
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2420    2420 
   Size:       4925
 
XmaI C'CCGG_G

Cuts at:   1945    1945 
   Size:       4925
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI    BsaXI     BsgI    BsiWI    BsmBI    BsrGI   BssHII
   BstEII  BstZ17I   Bsu36I    CspCI   DraIII     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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