Restriction map for pF1KB5090
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccI        AccII       AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BanII       BbeI        BbrPI       BcgI        
BciVI       BfrI        BlnI        BlpI        Bpu1102I    
BsaBI       BsiWI       BsmBI       BsoBI       BspDI       
BspHI       BsrDI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    EcoNI       EcoO65I     
EcoRV       FseI        FspI        HindIII     HpaI        
KasI        KspI        MamI        MluI        MluNI       
MscI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        Tth111I     XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I         38  GGTACC               G^GTACC
AccIII        452  TCCGGA               T^CCGGA
AflIII       1940  ACATGT               A^C[GA][CT]GT
AocI         1236  CCTGAGG              CC^T[ACGT]AGG
Asp718I        38  GGTACC               G^GTACC
AspHI        1794  GAGCAC               G[AT]GC[AT]^C
BamHI        1138  GGATCC               G^GATCC
BbsI         2115  GAAGAC               GAAGAC(2/6)
BbvCI        2234  CCTCAGC              CCTCAGC(-5/-2)
BclI         1313  TGATCA               T^GATCA
BglI          358  GCCACTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         558  AGATCT               A^GATCT
BpuAI        2115  GAAGAC               GAAGAC(2/6)
BsaAI         688  TACGTG               [CT]AC^GT[GA]
BsaWI         452  TCCGGA               [AT]^CCGG[AT]
BseAI         452  TCCGGA               T^CCGGA
BsiEI         904  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1794  GAGCAC               G[AT]GC[AT]^C
BsmI         2044  GCATTC               GAATGC(1/-1)
Bsp1407I      894  TGTACA               T^GTACA
BspEI         452  TCCGGA               T^CCGGA
BspLU11I     1940  ACATGT               A^CATGT
BsrGI         894  TGTACA               T^GTACA
Bsu36I       1236  CCTGAGG              CC^T[ACGT]AGG
EagI          904  CGGCCG               C^GGCCG
EclXI         904  CGGCCG               C^GGCCG
Eco52I        904  CGGCCG               C^GGCCG
Eco81I       1236  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1219  GAATTC               G^AATTC
EcoT22I       991  ATGCAT               ATGCA^T
FbaI         1313  TGATCA               T^GATCA
HaeII        2098  GGCGCT               [GA]GCGC^[CT]
KpnI           38  GGTACC               GGTAC^C
MroI          452  TCCGGA               T^CCGGA
NdeI          131  CATATG               CA^TATG
NsiI          991  ATGCAT               ATGCA^T
PshAI        1020  GACCGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        1602  ACCTGGT              A^CC[AT]GGT
SphI          983  GCATGC               GCATG^C
SspBI         894  TGTACA               T^GTACA
StuI         1344  AGGCCT               AGG^CCT
XbaI          471  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BmyI         1161  GGGCAC               G[AGT]GC[ACT]^C
             1794  GAGCAC              
BsaI         1324  GAGACC               GGTCTC(1/5)
             1363  GAGACC              
Bsp1286I     1161  GGGCAC               G[AGT]GC[ACT]^C
             1794  GAGCAC              
BstXI        1411  CCAAATGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1988  CCAATATCCTGG        
DsaI          404  CCGTGG               C^C[GA][CT]GG
             1907  CCGTGG              
EarI          456  GAAGAG               CTCTTC(1/4)
              729  GAAGAG              
HincII        833  GTCAAC               GT[CT]^[GA]AC
             2277  GTTGAC              
HindII        833  GTCAAC               GT[CT]^[GA]AC
             2277  GTTGAC              
Ksp632I       456  GAAGAG               CTCTTC(1/4)
              729  GAAGAG              
MfeI         1272  CAATTG               C^AATTG
             1490  CAATTG              
MunI         1272  CAATTG               C^AATTG
             1490  CAATTG              
PflMI        1427  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1902  CCAGTCCGTGG         
TaqI          223  TCGA                 T^CGA
             1565  TCGA                
TthHB8I       223  TCGA                 T^CGA
             1565  TCGA                
Van91I       1427  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1902  CCAGTCCGTGG         
XcmI          395  CCACAGCAGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1489  CCAATTGGAACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaII       BanI        
BbvI        BcnI        BfaI        BpmI        BsaHI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
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