Restriction map for pF1KB5090
 (Linear) (Six-base) MAPSORT of: KIBB5090.seq  Check: 2653  from: 1  to: 2307



KIBB5090.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

Acc65I G'GTAC_C

Cuts at:      0      38    2307 
   Size:         38    2269
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     427     808    2105    2134    2307 
   Size:        253     174     381    1297      29     173

  Fragments arranged by size: 

                1297     381     253     174     173      29
 
AflIII A'CryG_T

Cuts at:      0    1940    2307 
   Size:       1940     367
 
AleI CACnn'nnGTG

Cuts at:      0     163    1174    1867    2307 
   Size:        163    1011     693     440

  Fragments arranged by size: 

                1011     693     440     163
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     761    1006    1834    2307 
   Size:        761     245     828     473

  Fragments arranged by size: 

                 828     761     473     245
 
AlwNI CAG_nnn'CTG

Cuts at:      0      99     361     429     606    1932    2101    2307 
   Size:         99     262      68     177    1326     169     206

  Fragments arranged by size: 

                1326     262     206     177     169      99      68
 
ApoI r'AATT_y

Cuts at:      0    1060    1147    1219    1642    2307 
   Size:       1060      87      72     423     665

  Fragments arranged by size: 

                1060     665     423      87      72
 
BamHI G'GATC_C

Cuts at:      0    1138    2307 
   Size:       1138    1169
 
BanI G'GyrC_C

Cuts at:      0      38     320    1810    2307 
   Size:         38     282    1490     497

  Fragments arranged by size: 

                1490     497     282      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2122    2307 
   Size:       2122     185
 
BbvCI CC'TCA_GC

Cuts at:      0    2235    2307 
   Size:       2235      72
 
BclI T'GATC_A

Cuts at:      0    1313    2307 
   Size:       1313     994
 
BfrBI ATG'CAT

Cuts at:      0     993    2307 
   Size:        993    1314
 
BglI GCCn_nnn'nGGC

Cuts at:      0     364    2307 
   Size:        364    1943
 
BglII A'GATC_T

Cuts at:      0     558    2307 
   Size:        558    1749
 
Bme1580I G_kGCm'C

Cuts at:      0    1165    2307 
   Size:       1165    1142
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1386    2298    2307 
   Size:       1386     912       9

  Fragments arranged by size: 

                1386     912       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     451     487     871     934    1790    2307 
   Size:         64     387      36     384      63     856     517

  Fragments arranged by size: 

                 856     517     387     384      64      63      36
 
Bpu10I CC'TnA_GC

Cuts at:      0    1522    2235    2307 
   Size:       1522     713      72

  Fragments arranged by size: 

                1522     713      72
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     520    1333    1812    2307 
   Size:        304     216     813     479     495

  Fragments arranged by size: 

                 813     495     479     304     216
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1318    1357    2307 
   Size:       1318      39     950

  Fragments arranged by size: 

                1318     950      39
 
BsaAI yAC'GTr

Cuts at:      0     690    2307 
   Size:        690    1617
 
BsaHI Gr'CG_yC

Cuts at:      0    1025    1423    1853    2307 
   Size:       1025     398     430     454

  Fragments arranged by size: 

                1025     454     430     398
 
BsaWI w'CCGG_w

Cuts at:      0     452    2307 
   Size:        452    1855
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1788    1818    2307 
   Size:       1788      30     489

  Fragments arranged by size: 

                1788     489      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     727     868     931    1315    1527    2053    2307 
   Size:        727     141      63     384     212     526     254

  Fragments arranged by size: 

                 727     526     384     254     212     141      63
 
BseYI C'CCAG_C

Cuts at:      0     368    2307 
   Size:        368    1939
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     712    1291    2307 
   Size:        484     228     579    1016

  Fragments arranged by size: 

                1016     579     484     228
 
BsiEI CG_ry'CG

Cuts at:      0     907    2307 
   Size:        907    1400
 
BsiHKAI G_wGCw'C

Cuts at:      0    1798    2307 
   Size:       1798     509
 
BsmI GAATG_Cn'

Cuts at:      0    2044    2307 
   Size:       2044     263
 
Bsp1286I G_dGCh'C

Cuts at:      0    1165    1798    2307 
   Size:       1165     633     509

  Fragments arranged by size: 

                1165     633     509
 
BspEI T'CCGG_A

Cuts at:      0     452    2307 
   Size:        452    1855
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266    2155    2174    2307 
   Size:        266    1889      19     133

  Fragments arranged by size: 

                1889     266     133      19
 
BsrBI CCG'CTC

Cuts at:      0     972    1776    1782    2307 
   Size:        972     804       6     525

  Fragments arranged by size: 

                 972     804     525       6
 
BsrFI r'CCGG_y

Cuts at:      0     902    1001    1021    2307 
   Size:        902      99      20    1286

  Fragments arranged by size: 

                1286     902      99      20
 
BsrGI T'GTAC_A

Cuts at:      0     894    2307 
   Size:        894    1413
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     361     426     597    2307 
   Size:        361      65     171    1710

  Fragments arranged by size: 

                1710     361     171      65
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1418    1995    2307 
   Size:       1418     577     312

  Fragments arranged by size: 

                1418     577     312
 
BstYI r'GATC_y

Cuts at:      0     468     558    1138    1898    2307 
   Size:        468      90     580     760     409

  Fragments arranged by size: 

                 760     580     468     409      90
 
Bsu36I CC'TnA_GG

Cuts at:      0    1237    2307 
   Size:       1237    1070
 
BtgI C'CryG_G

Cuts at:      0     404    1907    2307 
   Size:        404    1503     400

  Fragments arranged by size: 

                1503     404     400
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     694    2307 
   Size:        694    1613
 
BtsI GCAGTG_nn'

Cuts at:      0     359     620    2307 
   Size:        359     261    1687

  Fragments arranged by size: 

                1687     359     261
 
EaeI y'GGCC_r

Cuts at:      0     137     904    1511    2062    2307 
   Size:        137     767     607     551     245

  Fragments arranged by size: 

                 767     607     551     245     137
 
EagI C'GGCC_G

Cuts at:      0     904    2307 
   Size:        904    1403
 
EarI CTCTTCn'nnn_

Cuts at:      0     451     724    2307 
   Size:        451     273    1583

  Fragments arranged by size: 

                1583     451     273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     253     427     451     487     808     871     934 
   Size:         64     189     174      24      36     321      63      63

Cuts at:    934    1790    2105    2134    2307 
   Size:        856     315      29     173

  Fragments arranged by size: 

                 856     321     315     189     174     173      64      63
                  63      36      29      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     885    1518    1600    2307 
   Size:        885     633      82     707

  Fragments arranged by size: 

                 885     707     633      82
 
EcoRI G'AATT_C

Cuts at:      0    1219    2307 
   Size:       1219    1088
 
HaeII r_GCGC'y

Cuts at:      0    2102    2307 
   Size:       2102     205
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     102     134     264     296    1096    1128    1440    1472 
   Size:        102      32     130      32     800      32     312      32

Cuts at:   1472    1547    1579    2307 
   Size:         75      32     728

  Fragments arranged by size: 

                 800     728     312     130     102      75      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     835    2279    2307 
   Size:        835    1444      28

  Fragments arranged by size: 

                1444     835      28
 
KpnI G_GTAC'C

Cuts at:      0      42    2307 
   Size:         42    2265
 
MfeI C'AATT_G

Cuts at:      0    1272    1490    2307 
   Size:       1272     218     817

  Fragments arranged by size: 

                1272     817     218
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1220    1418    2307 
   Size:       1220     198     889

  Fragments arranged by size: 

                1220     889     198
 
MslI CAynn'nnrTG

Cuts at:      0     163     988    1174    1867    2307 
   Size:        163     825     186     693     440

  Fragments arranged by size: 

                 825     693     440     186     163
 
MspA1I CmG'CkG

Cuts at:      0     606    1572    1886    1932    2307 
   Size:        606     966     314      46     375

  Fragments arranged by size: 

                 966     606     375     314      46
 
NdeI CA'TA_TG

Cuts at:      0     132    2307 
   Size:        132    2175
 
NsiI A_TGCA'T

Cuts at:      0     995    2307 
   Size:        995    1312
 
NspI r_CATG'y

Cuts at:      0     157     555     987    1446    1944    2307 
   Size:        157     398     432     459     498     363

  Fragments arranged by size: 

                 498     459     432     398     363     157
 
PciI A'CATG_T

Cuts at:      0    1940    2307 
   Size:       1940     367
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1433    1908    2307 
   Size:       1433     475     399

  Fragments arranged by size: 

                1433     475     399
 
PfoI T'CCnGG_A

Cuts at:      0     503    1141    2307 
   Size:        503     638    1166

  Fragments arranged by size: 

                1166     638     503
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     602     634    2307 
   Size:        602      32    1673

  Fragments arranged by size: 

                1673     602      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     885    1518    1600    2307 
   Size:        885     633      82     707

  Fragments arranged by size: 

                 885     707     633      82
 
PshAI GACnn'nnGTC

Cuts at:      0    1024    2307 
   Size:       1024    1283
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1732    1764    2307 
   Size:       1732      32     543

  Fragments arranged by size: 

                1732     543      32
 
PstI C_TGCA'G

Cuts at:      0     357    1218    2152    2307 
   Size:        357     861     934     155

  Fragments arranged by size: 

                 934     861     357     155
 
PvuII CAG'CTG

Cuts at:      0     606    1572    1932    2307 
   Size:        606     966     360     375

  Fragments arranged by size: 

                 966     606     375     360
 
SanDI GG'GwC_CC

Cuts at:      0     885    2307 
   Size:        885    1422
 
SexAI A'CCwGG_T

Cuts at:      0    1602    2307 
   Size:       1602     705
 
SfcI C'TryA_G

Cuts at:      0      19      63     346     353    1214    2148    2307 
   Size:         19      44     283       7     861     934     159

  Fragments arranged by size: 

                 934     861     283     159      44      19       7
 
SmlI C'TyrA_G

Cuts at:      0     283     535    1348    1791    2307 
   Size:        283     252     813     443     516

  Fragments arranged by size: 

                 813     516     443     283     252
 
SphI G_CATG'C

Cuts at:      0     987    2307 
   Size:        987    1320
 
StuI AGG'CCT

Cuts at:      0    1346    2307 
   Size:       1346     961
 
StyI C'CwwG_G

Cuts at:      0     106    1341    2033    2307 
   Size:        106    1235     692     274

  Fragments arranged by size: 

                1235     692     274     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1342    1857    2307 
   Size:       1342     515     450

  Fragments arranged by size: 

                1342     515     450
 
TatI w'GTAC_w

Cuts at:      0     894    1721    2307 
   Size:        894     827     586

  Fragments arranged by size: 

                 894     827     586
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1592    1624    1788    1820    2307 
   Size:       1592      32     164      32     487

  Fragments arranged by size: 

                1592     487     164      32      32
 
XbaI T'CTAG_A

Cuts at:      0     471    2307 
   Size:        471    1836
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     402    1496    2307 
   Size:        402    1094     811

  Fragments arranged by size: 

                1094     811     402
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI    BamHI
     BanI     BbsI    BbvCI     BclI    BfrBI     BglI    BglII Bme1580I
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EagI     EarI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I   HincII     KpnI     MfeI     MmeI
     MslI   MspA1I     NdeI     NsiI     NspI     PciI    PflMI     PfoI
     PpiI    PpuMI    PshAI     PsrI     PstI    PvuII    SanDI    SexAI
     SfcI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BanII     BbeI     BcgI    BciVI     BlpI    BmgBI     BmrI
     BmtI    BsaBI    BsiWI    BsmBI    BspHI    BsrDI   BssHII    BssSI
    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XhoI     XmaI
     XmnI     ZraI
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