Restriction map for pF1KB5072
 (Circular) (Six-base) MAPSORT of: pF1KB5072.seq  Check: 9830  from: 1  to: 4961



pF1KB5072  4961 bp

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:   1058    1999    1058 
   Size:        941    4020
 
Acc65I G'GTAC_C

Cuts at:   1977    1977 
   Size:       4961
 
AclI AA'CG_TT

Cuts at:   4801    4801 
   Size:       4961
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    443     913    1736    2786    3218    3965    4233     443 
   Size:        470     823    1050     432     747     268    1171

  Fragments arranged by size: 

                1171    1050     823     747     470     432     268
 
AflIII A'CryG_T

Cuts at:   2313    3417    4394    2313 
   Size:       1104     977    2880

  Fragments arranged by size: 

                2880    1104     977
 
AgeI A'CCGG_T

Cuts at:   3349    3349 
   Size:       4961
 
AleI CACnn'nnGTG

Cuts at:   1510    1510 
   Size:       4961
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    469     469 
   Size:       4961
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    250     282     250 
   Size:         32    4929
 
AlwNI CAG_nnn'CTG

Cuts at:    329    2266    3833     329 
   Size:       1937    1567    1457

  Fragments arranged by size: 

                1937    1567    1457
 
ApaLI G'TGCA_C

Cuts at:   3731    3731 
   Size:       4961
 
ApoI r'AATT_y

Cuts at:   1207    1624    1965    4314    4570    1207 
   Size:        417     341    2349     256    1598

  Fragments arranged by size: 

                2349    1598     417     341     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4961
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4961
 
AvaI C'yCGr_G

Cuts at:    472     552     752    1030    1250    1981     472 
   Size:         80     200     278     220     731    3452

  Fragments arranged by size: 

                3452     731     278     220     200      80
 
BamHI G'GATC_C

Cuts at:   1986    1986 
   Size:       4961
 
BanI G'GyrC_C

Cuts at:    660     886    1977    2391    2639    2674     660 
   Size:        226    1091     414     248      35    2947

  Fragments arranged by size: 

                2947    1091     414     248     226      35
 
BanII G_rGCy'C

Cuts at:   1251    1975    3005    1251 
   Size:        724    1030    3207

  Fragments arranged by size: 

                3207    1030     724
 
BbeI G_GCGC'C

Cuts at:   2395    2643    2395 
   Size:        248    4713
 
BbsI GAAGACnn'nnnn_

Cuts at:   1094    1283    1094 
   Size:        189    4772
 
BciVI GTATCCnnnnn_n'

Cuts at:    153    2859    3620    4961     153 
   Size:       2706     761    1341     153

  Fragments arranged by size: 

                2706    1341     761     153
 
BclI T'GATC_A

Cuts at:   1581    2481    1581 
   Size:        900    4061
 
BglI GCCn_nnn'nGGC

Cuts at:    139     139 
   Size:       4961
 
BglII A'GATC_T

Cuts at:   2476    4513    2476 
   Size:       2037    2924
 
BlpI GC'TnA_GC

Cuts at:    121    2073     121 
   Size:       1952    3009
 
Bme1580I G_kGCm'C

Cuts at:    458     665    2586    2679    3735     458 
   Size:        207    1921      93    1056    1684

  Fragments arranged by size: 

                1921    1684    1056     207      93
 
BmgBI CAC'GTC

Cuts at:   1887    1887 
   Size:       4961
 
BmrI ACTGGGnnnn_n'

Cuts at:   2173    2254    2589    4733    2173 
   Size:         81     335    2144    2401

  Fragments arranged by size: 

                2401    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3328    3328 
   Size:       4961
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    479     762     479 
   Size:        283    4678
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2137    3016    3508    3806    2137 
   Size:        879     492     298    3292

  Fragments arranged by size: 

                3292     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1837    4665    1837 
   Size:       2828    2133
 
BsaAI yAC'GTr

Cuts at:   2314    2314 
   Size:       4961
 
BsaBI GATnn'nnATC

Cuts at:      6    2475    4512       6 
   Size:       2469    2037     455

  Fragments arranged by size: 

                2469    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1091    2392    2640    3342    4838    1091 
   Size:       1301     248     702    1496    1214

  Fragments arranged by size: 

                1496    1301    1214     702     248
 
BsaWI w'CCGG_w

Cuts at:   2150    2671    3349    3623    3770    4181    2150 
   Size:        521     678     274     147     411    2930

  Fragments arranged by size: 

                2930     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1238    1268    1238 
   Size:         30    4931
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    482     482 
   Size:       4961
 
BseYI C'CCAG_C

Cuts at:    513     533     619    2386    3144    3721    4217     513 
   Size:         20      86    1767     758     577     496    1257

  Fragments arranged by size: 

                1767    1257     758     577     496      86      20
 
BsiEI CG_ry'CG

Cuts at:    104    2503    2549    3757     104 
   Size:       2399      46    1208    1308

  Fragments arranged by size: 

                2399    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    711    1975    2753    2943    3735     711 
   Size:       1264     778     190     792    1937

  Fragments arranged by size: 

                1937    1264     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1803    1803 
   Size:       4961
 
Bsp1286I G_dGCh'C

Cuts at:    458     665     711    1251    1975    2586    2679    2753    2943 
   Size:        207      46     540     724     611      93      74     190

Cuts at:   2943    3005    3735     458 
   Size:         62     730    1684

  Fragments arranged by size: 

                1684     730     724     611     540     207     190      93
                  74      62      46
 
BspEI T'CCGG_A

Cuts at:   4181    4181 
   Size:       4961
 
BspHI T'CATG_A

Cuts at:    242    1928    4137     242 
   Size:       1686    2209    1066

  Fragments arranged by size: 

                2209    1686    1066
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    604    2011    2225    2527    2908     604 
   Size:       1407     214     302     381    2657

  Fragments arranged by size: 

                2657    1407     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1403    3253    3307    4791    4956      50 
   Size:       1353    1850      54    1484     165      55

  Fragments arranged by size: 

                1850    1484    1353     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    134     665    1304    2873     134 
   Size:        531     639    1569    2222

  Fragments arranged by size: 

                2222    1569     639     531
 
BsrFI r'CCGG_y

Cuts at:   2959    3140    3349    2959 
   Size:        181     209    4571

  Fragments arranged by size: 

                4571     209     181
 
BssSI C'ACGA_G

Cuts at:    301     710    3232    3590     301 
   Size:        409    2522     358    1672

  Fragments arranged by size: 

                2522    1672     409     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2338    2338 
   Size:       4961
 
BstBI TT'CG_AA

Cuts at:   3322    3322 
   Size:       4961
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1881    1881 
   Size:       4961
 
BstYI r'GATC_y

Cuts at:      1     731    1070    1986    2453    2476    2811    3057    4069 
   Size:        730     339     916     467      23     335     246    1012

Cuts at:   4069    4155    4167    4513       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     916     730     467     449     346     339     335
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107    1895     107 
   Size:       1788    3173
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2911    3092    3255    2911 
   Size:        181     163    4617

  Fragments arranged by size: 

                4617     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2204    2204 
   Size:       4961
 
DraI TTT'AAA

Cuts at:   1960    1960 
   Size:       4961
 
DrdI GACnn_nn'nnGTC

Cuts at:   2667    3525    2667 
   Size:        858    4103
 
EaeI y'GGCC_r

Cuts at:    114     140    1502    1914    2546    2720    3111    3138    4483 
   Size:         26    1362     412     632     174     391      27    1345

Cuts at:   4483     114 
   Size:        592

  Fragments arranged by size: 

                1362    1345     632     592     412     391     174      27
                  26
 
EagI C'GGCC_G

Cuts at:   2546    2546 
   Size:       4961
 
EarI CTCTTCn'nnn_

Cuts at:   2984    3194    2984 
   Size:        210    4751
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    212    1421    3169    3479    3625    4771     212 
   Size:       1209    1748     310     146    1146     402

  Fragments arranged by size: 

                1748    1209    1146     402     310     146
 
EcoICRI GAG'CTC

Cuts at:   1973    1973 
   Size:       4961
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    443     479     762     913    1736    2786    3218    3965    4233 
   Size:         36     283     151     823    1050     432     747     268

Cuts at:   4233     443 
   Size:       1171

  Fragments arranged by size: 

                1171    1050     823     747     432     283     268     151
                  36
 
EcoO109I rG'GnC_Cy

Cuts at:    530     616    2100     530 
   Size:         86    1484    3391

  Fragments arranged by size: 

                3391    1484      86
 
EcoRI G'AATT_C

Cuts at:   1965    1965 
   Size:       4961
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    784    1069    1741    2980     784 
   Size:        285     672    1239    2765

  Fragments arranged by size: 

                2765    1239     672     285
 
FspI TGC'GCA

Cuts at:   2742    2742 
   Size:       4961
 
HaeII r_GCGC'y

Cuts at:    502    2395    2643    3665    4644     502 
   Size:       1893     248    1022     979     819

  Fragments arranged by size: 

                1893    1022     979     819     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    374     406     432     464    1024    1056    1238    1262    1270 
   Size:         32      26      32     560      32     182      24       8

Cuts at:   1270    1294    1438    1470    1817    1849    2803    2833    2835 
   Size:         24     144      32     347      32     954      30       2

Cuts at:   2835    2865     374 
   Size:         30    2470

  Fragments arranged by size: 

                2470     954     560     347     182     144      32      32
                  32      32      32      30      30      26      24      24
                   8       2
 
HincII GTy'rAC

Cuts at:    789    2000     789 
   Size:       1211    3750
 
HindIII A'AGCT_T

Cuts at:    766    1336     766 
   Size:        570    4391
 
KasI G'GCGC_C

Cuts at:   2391    2639    2391 
   Size:        248    4713
 
KpnI G_GTAC'C

Cuts at:   1981    1981 
   Size:       4961
 
MfeI C'AATT_G

Cuts at:    316     316 
   Size:       4961
 
MluI A'CGCG_T

Cuts at:   4394    4394 
   Size:       4961
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    411    1392    3632    3816     411 
   Size:        981    2240     184    1556

  Fragments arranged by size: 

                2240    1556     981     184
 
MscI TGG'CCA

Cuts at:   1504    2722    1504 
   Size:       1218    3743
 
MslI CAynn'nnrTG

Cuts at:   1510    1879    2950    3077    1510 
   Size:        369    1071     127    3394

  Fragments arranged by size: 

                3394    1071     369     127
 
MspA1I CmG'CkG

Cuts at:    120     421     919    1604    2072    2386    2746    3759    4004 
   Size:        301     498     685     468     314     360    1013     245

Cuts at:   4004     120 
   Size:       1077

  Fragments arranged by size: 

                1077    1013     685     498     468     360     314     301
                 245
 
NaeI GCC'GGC

Cuts at:   3142    3142 
   Size:       4961
 
NarI GG'CG_CC

Cuts at:   2392    2640    2392 
   Size:        248    4713
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4961
 
NgoMIV G'CCGG_C

Cuts at:   3140    3140 
   Size:       4961
 
NspI r_CATG'y

Cuts at:   1539    2014    2514    3421    1539 
   Size:        475     500     907    3079

  Fragments arranged by size: 

                3079     907     500     475
 
PciI A'CATG_T

Cuts at:   3417    3417 
   Size:       4961
 
PfoI T'CCnGG_A

Cuts at:   1162    1703    1162 
   Size:        541    4420
 
PmeI GTTT'AAAC

Cuts at:   1960    1960 
   Size:       4961
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4100    4132    4100 
   Size:         32    4929
 
PpuMI rG'GwC_Cy

Cuts at:    616     616 
   Size:       4961
 
PshAI GACnn'nnGTC

Cuts at:    961     961 
   Size:       4961
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1522    1554    1522 
   Size:         32    4929
 
PstI C_TGCA'G

Cuts at:    615    2008    2693     615 
   Size:       1393     685    2883

  Fragments arranged by size: 

                2883    1393     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4961
 
PvuII CAG'CTG

Cuts at:    120     421     919    2386    2746     120 
   Size:        301     498    1467     360    2335

  Fragments arranged by size: 

                2335    1467     498     360     301
 
RsrII CG'GwC_CG

Cuts at:   3156    3156 
   Size:       4961
 
SacI G_AGCT'C

Cuts at:   1975    1975 
   Size:       4961
 
SalI G'TCGA_C

Cuts at:   1998    1998 
   Size:       4961
 
SapI GCTCTTCn'nnn_

Cuts at:   2984    3194    2984 
   Size:        210    4751
 
SbfI CC_TGCA'GG

Cuts at:   2008    2008 
   Size:       4961
 
ScaI AGT'ACT

Cuts at:   4478    4478 
   Size:       4961
 
SfcI C'TryA_G

Cuts at:     33     611     812    1902    2004    2689    3682    3873      33 
   Size:        578     201    1090     102     685     993     191    1121

  Fragments arranged by size: 

                1121    1090     993     685     578     201     191     102
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    139     139 
   Size:       4961
 
SfoI GGC'GCC

Cuts at:   2393    2641    2393 
   Size:        248    4713
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4961
 
SmaI CCC'GGG

Cuts at:   1983    1983 
   Size:       4961
 
SmlI C'TyrA_G

Cuts at:   1030    2116    3031    3523    3785    1030 
   Size:       1086     915     492     262    2206

  Fragments arranged by size: 

                2206    1086     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4961
 
SphI G_CATG'C

Cuts at:   2014    2514    2014 
   Size:        500    4461
 
SspI AAT'ATT

Cuts at:   1665    1665 
   Size:       4961
 
StuI AGG'CCT

Cuts at:   1330    1330 
   Size:       4961
 
StyI C'CwwG_G

Cuts at:    107    1470    1725    2095    2449     107 
   Size:       1363     255     370     354    2619

  Fragments arranged by size: 

                2619    1363     370     354     255
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1680    2544    2677    3136    3345    1680 
   Size:        864     133     459     209    3296

  Fragments arranged by size: 

                3296     864     459     209     133
 
TatI w'GTAC_w

Cuts at:   4476    4476 
   Size:       4961
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    690     722    1611    1643    4478    4510     690 
   Size:         32     889      32    2835      32    1141

  Fragments arranged by size: 

                2835    1141     889      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2758    2758 
   Size:       4961
 
XbaI T'CTAG_A

Cuts at:   1219    1992    1219 
   Size:        773    4188
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    541    2456     541 
   Size:       1915    3046
 
XhoI C'TCGA_G

Cuts at:   1030    1030 
   Size:       4961
 
XmaI C'CCGG_G

Cuts at:   1981    1981 
   Size:       4961
 
XmnI GAAnn'nnTTC

Cuts at:    561     769     561 
   Size:        208    4753
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     ApaI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BmtI   Bpu10I     BsgI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI     PsiI
   PspOMI    PspXI    SacII    SanDI    SexAI    SgrAI    SnaBI     SrfI
     SwaI     ZraI
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