Restriction map for pF1KB5068
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       BamHI       BbeI        BbrPI       BbvCI       
BciVI       BclI        BglI        BglII       BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsgI        BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstYI       BstZ17I     
CelII       ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco47III    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HaeII       Hin1I       HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspI        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Van91I      
XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          610  GTAC                 GT^AC
AocI         1096  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1354  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1589  TGCGCA               TGC^GCA
AvrII        1402  CCTAGG               C^CTAGG
BalI          344  TGGCCA               TGG^CCA
BanI          890  GGTGCC               G^G[CT][GA]CC
BcgI         1317  GCAGGCCATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         1402  CCTAGG               C^CTAGG
BsiEI         173  CGGCCG               CG[GA][CT]^CG
BsmI         1365  GCATTC               GAATGC(1/-1)
BspMI        1027  GCAGGT               ACCTGC(4/8)
BsrFI         815  ACCGGC               [GA]^CCGG[CT]
BstBI          25  TTCGAA               TT^CGAA
BstEII        645  GGTTACC              G^GT[ACGT]ACC
BstPI         645  GGTTACC              G^GT[ACGT]ACC
Bsu36I       1096  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        815  ACCGGC               [GA]^CCGG[CT]
DraIII       1054  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          173  CGGCCG               C^GGCCG
EclXI         173  CGGCCG               C^GGCCG
Eco52I        173  CGGCCG               C^GGCCG
Eco81I       1096  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1547  CCTCTTGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       645  GGTTACC              G^GT[ACGT]ACC
FspI         1589  TGCGCA               TGC^GCA
HgaI          981  GCGTC                GACGC(5/10)
HincII        793  GTCAAC               GT[CT]^[GA]AC
HindII        793  GTCAAC               GT[CT]^[GA]AC
HindIII      1375  AAGCTT               A^AGCTT
MaeII         608  ACGT                 A^CGT
MluNI         344  TGGCCA               TGG^CCA
MscI          344  TGGCCA               TGG^CCA
MslI          852  CACCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI           82  TCGCGA               TCG^CGA
NspV           25  TTCGAA               TT^CGAA
RsaI          610  GTAC                 GT^AC
SacI          535  GAGCTC               GAGCT^C
SexAI        1484  ACCTGGT              A^CC[AT]GGT
SfuI           25  TTCGAA               TT^CGAA
SmaI         1037  CCCGGG               CCC^GGG
XbaI          801  TCTAGA               T^CTAGA
XmaCI        1037  CCCGGG               C^CCGGG
XmaI         1037  CCCGGG               C^CCGGG
XmnI         1354  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          83  CGCG                 CG^CG
             1132  CGCG                
AccIII       1020  TCCGGA               T^CCGGA
             1253  TCCGGA              
AcsI          730  AAATTC               [GA]^AATT[CT]
             1615  AAATTC              
AflII         652  CTTAAG               C^TTAAG
             1378  CTTAAG              
ApoI          730  AAATTC               [GA]^AATT[CT]
             1615  AAATTC              
AspI          367  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              928  GACTGTGTC           
BfrI          652  CTTAAG               C^TTAAG
             1378  CTTAAG              
BseAI        1020  TCCGGA               T^CCGGA
             1253  TCCGGA              
BspEI        1020  TCCGGA               T^CCGGA
             1253  TCCGGA              
BstAPI        198  GCAGCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              940  GCAACTGATGC         
BstUI          83  CGCG                 CG^CG
             1132  CGCG                
DsaI         1172  CCACGG               C^C[GA][CT]GG
             1503  CCGTGG              
EaeI          173  CGGCCG               [CT]^GGCC[GA]
              344  TGGCCA              
EarI          532  GAAGAG               CTCTTC(1/4)
             1354  GAAGAG              
FauI          152  CCCGC                CCCGC(4/6)
             1444  CCCGC               
HphI         1452  GGTGA                GGTGA(8/7)
               65  TCACC               
Ksp632I       532  GAAGAG               CTCTTC(1/4)
             1354  GAAGAG              
MroI         1020  TCCGGA               T^CCGGA
             1253  TCCGGA              
MvnI           83  CGCG                 CG^CG
             1132  CGCG                
PflFI         367  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              928  GACTGTGTC           
PstI          196  CTGCAG               CTGCA^G
             1287  CTGCAG              
SapI          532  GAAGAGC              GCTCTTC(1/4)
             1354  GAAGAGC             
SfcI          196  CTGCAG               C^T[GA][CT]AG
             1287  CTGCAG              
TfiI          303  GATTC                G^A[AT]TC
              768  GATTC               
Tsp509I       731  AATT                 ^AATT
             1616  AATT                
Tth111I       367  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              928  GACTGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       ApaI        
AspHI       AvaI        AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     
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