Restriction map for pF1KB5053
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvrII       BbrPI       
BbvCI       BciVI       BclI        BfrI        BlnI        
BsaAI       BseRI       BsiHKAI     BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        DraI        
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     KspI        MfeI        MluI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          814  GTAGAC               GT^[AC][GT]AC
AccIII       1578  TCCGGA               T^CCGGA
AcsI          961  GAATTC               [GA]^AATT[CT]
AcyI            3  GGCGCC               G[GA]^CG[CT]C
ApaI         1051  GGGCCC               GGGCC^C
ApoI          961  GAATTC               [GA]^AATT[CT]
Asp700I      2187  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1895  TGCGCA               TGC^GCA
BbeI            3  GGCGCC               GGCGC^C
BbsI          937  GAAGAC               GAAGAC(2/6)
BcgI         1452  CGAGACCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1301  AGATCT               A^GATCT
BlpI         1184  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1184  GCTCAGC              GC^T[ACGT]AGC
BpuAI         937  GAAGAC               GAAGAC(2/6)
BsaBI        1749  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           3  GGCGCC               G[GA]^CG[CT]C
BseAI        1578  TCCGGA               T^CCGGA
BspEI        1578  TCCGGA               T^CCGGA
BsrBI        1282  GAGCGG               CCGCTC(-3/-3)
BssSI        2083  CACGAG               CACGAG(-5/-1)
BstXI         801  CCACGGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1184  GCTCAGC              GC^T[ACGT]AGC
CpoI          278  CGGACCG              CG^G[AT]CCG
EcoRI         961  GAATTC               G^AATTC
FspI         1895  TGCGCA               TGC^GCA
Hin1I           3  GGCGCC               G[GA]^CG[CT]C
KasI            3  GGCGCC               G^GCGCC
MamI         1749  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1578  TCCGGA               T^CCGGA
NarI            3  GGCGCC               GG^CGCC
NcoI         1215  CCATGG               C^CATGG
PaeR7I       2004  CTCGAG               C^TCGAG
RsrII         278  CGGACCG              CG^G[AT]CCG
SfiI          306  GGCCCAGATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI            3  GGCGCC               GGC^GCC
SphI         2016  GCATGC               GCATG^C
TaqI         2005  TCGA                 T^CGA
TthHB8I      2005  TCGA                 T^CGA
XhoI         2004  CTCGAG               C^TCGAG
XmnI         2187  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII        1270  CGCG                 CG^CG
             2299  CGCG                
AvaI         1450  CCCGAG               C^[CT]CG[GA]G
             2004  CTCGAG              
BamHI         656  GGATCC               G^GATCC
             2078  GGATCC              
BfaI          142  CTAG                 C^TAG
              952  CTAG                
BsaWI         715  TCCGGT               [AT]^CCGG[AT]
             1578  TCCGGA              
BsmBI        1982  GAGACG               CGTCTC(1/5)
             2278  GAGACG              
BsoBI        1450  CCCGAG               C^[CT]CG[GA]G
             2004  CTCGAG              
BsrFI         179  ACCGGC               [GA]^CCGG[CT]
              928  ACCGGC              
BstUI        1270  CGCG                 CG^CG
             2299  CGCG                
Cfr10I        179  ACCGGC               [GA]^CCGG[CT]
              928  ACCGGC              
EcoNI        1412  CCTACAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1752  CCTCATCAAGG         
HgaI         1441  GACGC                GACGC(5/10)
             2280  GACGC               
MaeI          142  CTAG                 C^TAG
              952  CTAG                
MaeII        1471  ACGT                 A^CGT
             1670  ACGT                
MseI          155  TTAA                 T^TAA
             1019  TTAA                
MvnI         1270  CGCG                 CG^CG
             2299  CGCG                
NspI         1662  GCATGT               [GA]CATG^[CT]
             2016  GCATGC              
PvuII        1190  CAGCTG               CAG^CTG
             1793  CAGCTG              
TfiI          512  GAATC                G^A[AT]TC
             2151  GATTC               
Tru9I         155  TTAA                 T^TAA
             1019  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BglI        BmyI        BpmI        BsaI        
BsaJI       BsgI        BsiEI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EagI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TseI        
Tsp45I      Tsp509I     TspRI       XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com