Restriction map for pF1KB5053
 (Linear) (Six-base) MAPSORT of: KIBB5053.seq  Check: 3822  from: 1  to: 2340



KIBB5053.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     460    2340 
   Size:        460    1880
 
AccI GT'mk_AC

Cuts at:      0     815    2340 
   Size:        815    1525
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     766    1054    2116    2206    2233    2340 
   Size:        691      75     288    1062      90      27     107

  Fragments arranged by size: 

                1062     691     288     107      90      75      27
 
AleI CACnn'nnGTG

Cuts at:      0     883    2340 
   Size:        883    1457
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1192    1209    1692    1701    1798    2109    2184    2340 
   Size:       1192      17     483       9      97     311      75     156

  Fragments arranged by size: 

                1192     483     311     156      97      75      17       9
            
 
ApaI G_GGCC'C

Cuts at:      0    1055    2340 
   Size:       1055    1285
 
ApoI r'AATT_y

Cuts at:      0     961    2340 
   Size:        961    1379
 
AvaI C'yCGr_G

Cuts at:      0    1450    2004    2340 
   Size:       1450     554     336

  Fragments arranged by size: 

                1450     554     336
 
BamHI G'GATC_C

Cuts at:      0     656    2078    2340 
   Size:        656    1422     262

  Fragments arranged by size: 

                1422     656     262
 
BanI G'GyrC_C

Cuts at:      0       3     472     628     978    1858    2340 
   Size:          3     469     156     350     880     482

  Fragments arranged by size: 

                 880     482     469     350     156       3
 
BanII G_rGCy'C

Cuts at:      0     116     709    1055    1678    2005    2340 
   Size:        116     593     346     623     327     335

  Fragments arranged by size: 

                 623     593     346     335     327     116
 
BbeI G_GCGC'C

Cuts at:      0       7    2340 
   Size:          7    2333
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     944    2340 
   Size:        944    1396
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1441    1475    2340 
   Size:       1441      34     865

  Fragments arranged by size: 

                1441     865      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      94     313     605    1204    2340 
   Size:         94     219     292     599    1136

  Fragments arranged by size: 

                1136     599     292     219      94
 
BglII A'GATC_T

Cuts at:      0    1301    2340 
   Size:       1301    1039
 
BlpI GC'TnA_GC

Cuts at:      0    1185    2340 
   Size:       1185    1155
 
Bme1580I G_kGCm'C

Cuts at:      0     679    1055    1549    1826    2313    2340 
   Size:        679     376     494     277     487      27

  Fragments arranged by size: 

                 679     494     487     376     277      27
 
BmgBI CAC'GTC

Cuts at:      0    1472    1671    2340 
   Size:       1472     199     669

  Fragments arranged by size: 

                1472     669     199
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1230    2340 
   Size:       1230    1110
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307    1235    1591    1723    1756    2047    2340 
   Size:        307     928     356     132      33     291     293

  Fragments arranged by size: 

                 928     356     307     293     291     132      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     919    2340 
   Size:        919    1421
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1207    1326    1498    1795    2340 
   Size:       1207     119     172     297     545

  Fragments arranged by size: 

                1207     545     297     172     119
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1447    1522    2080    2242    2340 
   Size:       1447      75     558     162      98

  Fragments arranged by size: 

                1447     558     162      98      75
 
BsaBI GATnn'nnATC

Cuts at:      0    1753    2340 
   Size:       1753     587
 
BsaHI Gr'CG_yC

Cuts at:      0       4    2340 
   Size:          4    2336
 
BsaWI w'CCGG_w

Cuts at:      0     715    1578    2340 
   Size:        715     863     762

  Fragments arranged by size: 

                 863     762     715
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     658     688     808     838    1233    1263    2340 
   Size:        658      30     120      30     395      30    1077

  Fragments arranged by size: 

                1077     658     395     120      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0     652    2340 
   Size:        652    1688
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     813     881    1137    2340 
   Size:        813      68     256    1203

  Fragments arranged by size: 

                1203     813     256      68
 
BsiEI CG_ry'CG

Cuts at:      0     184     277     933    2340 
   Size:        184      93     656    1407

  Fragments arranged by size: 

                1407     656     184      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1976    2272    2340 
   Size:       1976     296      68

  Fragments arranged by size: 

                1976     296      68
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     679     709    1055    1549    1678    1826    2005 
   Size:        116     563      30     346     494     129     148     179

Cuts at:   2005    2313    2340 
   Size:        308      27

  Fragments arranged by size: 

                 563     494     346     308     179     148     129     116
                  30      27
 
BspEI T'CCGG_A

Cuts at:      0    1578    2340 
   Size:       1578     762
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     146     272     425     460    1751    1856    2340 
   Size:        146     126     153      35    1291     105     484

  Fragments arranged by size: 

                1291     484     153     146     126     105      35
 
BsrBI CCG'CTC

Cuts at:      0    1284    2340 
   Size:       1284    1056
 
BsrDI GCAATG_nn'

Cuts at:      0     941    1499    1905    2340 
   Size:        941     558     406     435

  Fragments arranged by size: 

                 941     558     435     406
 
BsrFI r'CCGG_y

Cuts at:      0     179     928    2340 
   Size:        179     749    1412

  Fragments arranged by size: 

                1412     749     179
 
BssSI C'ACGA_G

Cuts at:      0    2083    2340 
   Size:       2083     257
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     808    2340 
   Size:        808    1532
 
BstYI r'GATC_y

Cuts at:      0     505     656    1265    1301    1311    1748    2078    2340 
   Size:        505     151     609      36      10     437     330     262

  Fragments arranged by size: 

                 609     505     437     330     262     151      36      10
            
 
BtgI C'CryG_G

Cuts at:      0     801    1215    1818    2340 
   Size:        801     414     603     522

  Fragments arranged by size: 

                 801     603     522     414
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     112    2340 
   Size:        112    2228
 
BtsI GCAGTG_nn'

Cuts at:      0    1934    2313    2340 
   Size:       1934     379      27

  Fragments arranged by size: 

                1934     379      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1323    1358    1800    1835    2340 
   Size:       1323      35     442      35     505

  Fragments arranged by size: 

                1323     505     442      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     978    1080    2160    2340 
   Size:        978     102    1080     180

  Fragments arranged by size: 

                1080     978     180     102
 
EaeI y'GGCC_r

Cuts at:      0     181     274     287     314     930    1196    1331    1356 
   Size:        181      93      13      27     616     266     135      25

Cuts at:   1356    1496    2340 
   Size:        140     844

  Fragments arranged by size: 

                 844     616     266     181     140     135      93      27
                  25      13
 
EagI C'GGCC_G

Cuts at:      0     181     274     930    2340 
   Size:        181      93     656    1410

  Fragments arranged by size: 

                1410     656     181      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     691     766    1054    1235    1591    1723    1756 
   Size:        307     384      75     288     181     356     132      33

Cuts at:   1756    2047    2116    2206    2233    2340 
   Size:        291      69      90      27     107

  Fragments arranged by size: 

                 384     356     307     291     288     181     132     107
                  90      75      69      33      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1416    1756    2340 
   Size:       1416     340     584

  Fragments arranged by size: 

                1416     584     340
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1052    1731    1740    2106    2181    2240    2340 
   Size:       1052     679       9     366      75      59     100

  Fragments arranged by size: 

                1052     679     366     100      75      59       9
 
EcoRI G'AATT_C

Cuts at:      0     961    2340 
   Size:        961    1379
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     990    2340 
   Size:        990    1350
 
FspI TGC'GCA

Cuts at:      0    1897    2340 
   Size:       1897     443
 
HaeII r_GCGC'y

Cuts at:      0       7    1185    1692    2340 
   Size:          7    1178     507     648

  Fragments arranged by size: 

                1178     648     507       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     497     529     693     725     808     840    1774    1806 
   Size:        497      32     164      32      83      32     934      32

Cuts at:   1806    1822    1854    2340 
   Size:         16      32     486

  Fragments arranged by size: 

                 934     497     486     164      83      32      32      32
                  32      32      16
 
KasI G'GCGC_C

Cuts at:      0       3    2340 
   Size:          3    2337
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1865    2340 
   Size:       1865     475
 
MscI TGG'CCA

Cuts at:      0     289     316    1198    1333    1358    1498    2340 
   Size:        289      27     882     135      25     140     842

  Fragments arranged by size: 

                 882     842     289     140     135      27      25
 
MslI CAynn'nnrTG

Cuts at:      0     221     835     883    1046    1132    2340 
   Size:        221     614      48     163      86    1208

  Fragments arranged by size: 

                1208     614     221     163      86      48
 
MspA1I CmG'CkG

Cuts at:      0     186     338    1192    1655    1795    2340 
   Size:        186     152     854     463     140     545

  Fragments arranged by size: 

                 854     545     463     186     152     140
 
NarI GG'CG_CC

Cuts at:      0       4    2340 
   Size:          4    2336
 
NcoI C'CATG_G

Cuts at:      0    1215    2340 
   Size:       1215    1125
 
NspI r_CATG'y

Cuts at:      0    1666    2020    2340 
   Size:       1666     354     320

  Fragments arranged by size: 

                1666     354     320
 
PasI CC'CwG_GG

Cuts at:      0     662    1615    2340 
   Size:        662     953     725

  Fragments arranged by size: 

                 953     725     662
 
PfoI T'CCnGG_A

Cuts at:      0    1700    2340 
   Size:       1700     640
 
PpuMI rG'GwC_Cy

Cuts at:      0    1731    1740    2240    2340 
   Size:       1731       9     500     100

  Fragments arranged by size: 

                1731     500     100       9
 
PsiI TTA'TAA

Cuts at:      0     452    2340 
   Size:        452    1888
 
PspOMI G'GGCC_C

Cuts at:      0    1051    2340 
   Size:       1051    1289
 
PspXI vC'TCGA_Gb

Cuts at:      0    2004    2340 
   Size:       2004     336
 
PstI C_TGCA'G

Cuts at:      0      35     471    1697    1748    2340 
   Size:         35     436    1226      51     592

  Fragments arranged by size: 

                1226     592     436      51      35
 
PvuII CAG'CTG

Cuts at:      0    1192    1795    2340 
   Size:       1192     603     545

  Fragments arranged by size: 

                1192     603     545
 
RsrII CG'GwC_CG

Cuts at:      0     279    2340 
   Size:        279    2061
 
SanDI GG'GwC_CC

Cuts at:      0    2240    2340 
   Size:       2240     100
 
SfcI C'TryA_G

Cuts at:      0       9      31     467    1693    1744    2340 
   Size:          9      22     436    1226      51     596

  Fragments arranged by size: 

                1226     596     436      51      22       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     313    2340 
   Size:        313    2027
 
SfoI GGC'GCC

Cuts at:      0       5    2340 
   Size:          5    2335
 
SmlI C'TyrA_G

Cuts at:      0    1222    1305    1513    1810    2004    2340 
   Size:       1222      83     208     297     194     336

  Fragments arranged by size: 

                1222     336     297     208     194      83
 
SphI G_CATG'C

Cuts at:      0    2020    2340 
   Size:       2020     320
 
StyI C'CwwG_G

Cuts at:      0      89     347     428     600     680    1215    1643    2040 
   Size:         89     258      81     172      80     535     428     397

Cuts at:   2040    2340 
   Size:        300

  Fragments arranged by size: 

                 535     428     397     300     258     172      89      81
                  80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      34     744     847    1306    1546    1652    1656    1959 
   Size:         34     710     103     459     240     106       4     303

Cuts at:   1959    2340 
   Size:        381

  Fragments arranged by size: 

                 710     459     381     303     240     106     103      34
                   4
 
TatI w'GTAC_w

Cuts at:      0     122    2340 
   Size:        122    2218
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     658     690    1233    1265    2340 
   Size:        658      32     543      32    1075

  Fragments arranged by size: 

                1075     658     543      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     483     586    1206    2340 
   Size:        483     103     620    1134

  Fragments arranged by size: 

                1134     620     483     103
 
XhoI C'TCGA_G

Cuts at:      0    2004    2340 
   Size:       2004     336
 
XmnI GAAnn'nnTTC

Cuts at:      0    2191    2340 
   Size:       2191     149
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI
    BstYI     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI
     PasI     PfoI    PpuMI     PsiI   PspOMI    PspXI     PstI    PvuII
    RsrII    SanDI     SfcI     SfiI     SfoI     SmlI     SphI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BbvCI    BciVI     BclI    BfrBI     BmtI     BplI    BsaAI    BseRI
  BsiHKAI    BsiWI     BsmI    BspHI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI     DraI     DrdI     EarI     EciI
  EcoICRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsrI     PvuI     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XmaI     ZraI
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