Restriction map for pF1KB5026
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NaeI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        Tsp45I      Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI          538  GTCTAC               GT^[AC][GT]AC
AccII          81  CGCG                 CG^CG
AcyI          969  GGCGCC               G[GA]^CG[CT]C
AflIII        771  ACATGT               A^C[GA][CT]GT
Alw44I         97  GTGCAC               G^TGCAC
ApaI         1336  GGGCCC               GGGCC^C
ApaLI          97  GTGCAC               G^TGCAC
Asp700I      1001  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          97  GTGCAC               G[AT]GC[AT]^C
AvaII         379  GGACC                G^G[AT]CC
BalI          474  TGGCCA               TGG^CCA
BanI          969  GGCGCC               G^G[CT][GA]CC
BanII        1336  GGGCCC               G[GA]GC[CT]^C
BbeI          969  GGCGCC               GGCGC^C
BbsI          441  GTCTTC               GAAGAC(2/6)
BbvI          395  GCAGC                GCAGC(8/12)
BpuAI         441  GTCTTC               GAAGAC(2/6)
BsaBI         196  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         969  GGCGCC               G[GA]^CG[CT]C
BseRI        1315  GAGGAG               GAGGAG(10/8)
BsgI         1258  GTGCAG               GTGCAG(16/14)
BsiHKAI        97  GTGCAC               G[AT]GC[AT]^C
BsmBI         845  GAGACG               CGTCTC(1/5)
BspLU11I      771  ACATGT               A^CATGT
BsrDI         238  GCAATG               GCAATG(2/0)
BstUI          81  CGCG                 CG^CG
BstXI         260  CCACAGTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          828  TTTAAA               TTT^AAA
DraIII       1188  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          474  TGGCCA               [CT]^GGCC[GA]
EarI          118  CTCTTC               CTCTTC(1/4)
EcoRV         500  GATATC               GAT^ATC
Hin1I         969  GGCGCC               G[GA]^CG[CT]C
KasI          969  GGCGCC               G^GCGCC
Ksp632I       118  CTCTTC               CTCTTC(1/4)
MaeIII        114  GTTAC                ^GT[ACGT]AC
MamI          196  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         474  TGGCCA               TGG^CCA
MscI          474  TGGCCA               TGG^CCA
MspA1I        919  CAGCTG               C[AC]G^C[GT]G
MvnI           81  CGCG                 CG^CG
NarI          969  GGCGCC               GG^CGCC
NcoI           32  CCATGG               C^CATGG
NheI            9  GCTAGC               G^CTAGC
NspI          771  ACATGT               [GA]CATG^[CT]
PvuII         919  CAGCTG               CAG^CTG
SexAI          41  ACCAGGT              A^CC[AT]GGT
SfaNI          59  GCATC                GCATC(5/9)
SfoI          969  GGCGCC               GGC^GCC
SspI          570  AATATT               AAT^ATT
StuI          807  AGGCCT               AGG^CCT
TaqI          204  TCGA                 T^CGA
TseI          395  GCAGC                G^C[AT]GC
TthHB8I       204  TCGA                 T^CGA
XmnI         1001  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          719  GTAC                 GT^AC
             1177  GTAC                
AvaI          371  CTCGGG               C^[CT]CG[GA]G
              558  CCCGAG              
BcnI          104  CCGGG                CC^[GC]GG
             1305  CCGGG               
BmyI           97  GTGCAC               G[AGT]GC[ACT]^C
             1336  GGGCCC              
BsmAI         845  GAGAC                GTCTC(1/5)
              406  GTCTC               
BsmFI         506  GGGAC                GGGAC(10/14)
              556  GTCCC               
BsoBI         371  CTCGGG               C^[CT]CG[GA]G
              558  CCCGAG              
Bsp1286I       97  GTGCAC               G[AGT]GC[ACT]^C
             1336  GGGCCC              
Bsp1407I      718  TGTACA               T^GTACA
             1176  TGTACA              
BsrGI         718  TGTACA               T^GTACA
             1176  TGTACA              
DraII        1335  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1336  GGGCCCC             
DsaI           32  CCATGG               C^C[GA][CT]GG
             1283  CCGTGG              
EcoO109I     1335  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1336  GGGCCCC             
HaeII         969  GGCGCC               [GA]GCGC^[CT]
             1308  GGCGCT              
MaeII         848  ACGT                 A^CGT
              943  ACGT                
NciI          104  CCGGG                CC^[GC]GG
             1305  CCGGG               
PflMI          42  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
               70  CCAAATAGTGG         
PstI          304  CTGCAG               CTGCA^G
             1114  CTGCAG              
RsaI          719  GTAC                 GT^AC
             1177  GTAC                
SspBI         718  TGTACA               T^GTACA
             1176  TGTACA              
Van91I         42  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
               70  CCAAATAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApoI        BfaI        BpmI        BsaJI       BsiYI       
BslI        BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfcI        StyI        TfiI        Tru9I       Tsp509I     
TspRI       XhoII       
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