Restriction map for pF1KB4980
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BbeI        BbrPI       
BcgI        BciVI       BfrI        BlnI        BsaAI       
BsaBI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tsp509I     Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI           58  GTCGAC               GT^[AC][GT]AC
AcyI          136  GACGCC               G[GA]^CG[CT]C
AflIII       2138  ACGTGT               A^C[GA][CT]GT
ApaI          908  GGGCCC               GGGCC^C
Asp700I      1437  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          175  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          758  CCCGGG               C^[CT]CG[GA]G
BamHI         599  GGATCC               G^GATCC
BclI         1610  TGATCA               T^GATCA
BglI         1736  GCCAGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1439  AGATCT               A^GATCT
BsaHI         136  GACGCC               G[GA]^CG[CT]C
BsmI         1049  GCATTC               GAATGC(1/-1)
BsoBI         758  CCCGGG               C^[CT]CG[GA]G
BsrDI        1716  CATTGC               GCAATG(2/0)
EcoNI         450  CCTGCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1610  TGATCA               T^GATCA
HgaI          136  GACGC                GACGC(5/10)
Hin1I         136  GACGCC               G[GA]^CG[CT]C
HindIII      2410  AAGCTT               A^AGCTT
MseI          389  TTAA                 T^TAA
NaeI          776  GCCGGC               GCC^GGC
NgoMIV        776  GCCGGC               G^CCGGC
PflFI         175  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI         1978  GAGCTC               GAGCT^C
SalI           58  GTCGAC               G^TCGAC
SapI         1158  GCTCTTC              GCTCTTC(1/4)
SmaI          758  CCCGGG               CCC^GGG
Tru9I         389  TTAA                 T^TAA
Tth111I       175  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         758  CCCGGG               C^CCGGG
XmaI          758  CCCGGG               C^CCGGG
XmnI         1437  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         962  CGCG                 CG^CG
             1309  CGCG                
AccIII       1586  TCCGGA               T^CCGGA
             1874  TCCGGA              
BbvCI         463  GCTGAGG              CCTCAGC(-5/-2)
             1381  GCTGAGG             
BlpI         1327  GCTGAGC              GC^T[ACGT]AGC
             1828  GCTCAGC             
Bpu1102I     1327  GCTGAGC              GC^T[ACGT]AGC
             1828  GCTCAGC             
BsaI          553  GAGACC               GGTCTC(1/5)
               18  GGTCTC              
BsaWI        1586  TCCGGA               [AT]^CCGG[AT]
             1874  TCCGGA              
BseAI        1586  TCCGGA               T^CCGGA
             1874  TCCGGA              
BsgI          484  CTGCAC               GTGCAG(16/14)
               43  GTGCAG              
BspEI        1586  TCCGGA               T^CCGGA
             1874  TCCGGA              
BsrBI        1114  GAGCGG               CCGCTC(-3/-3)
             1330  GAGCGG              
BsrFI         776  GCCGGC               [GA]^CCGG[CT]
             1604  ACCGGC              
BstUI         962  CGCG                 CG^CG
             1309  CGCG                
CelII        1327  GCTGAGC              GC^T[ACGT]AGC
             1828  GCTCAGC             
Cfr10I        776  GCCGGC               [GA]^CCGG[CT]
             1604  ACCGGC              
FauI          963  GCGGG                CCCGC(4/6)
             1332  GCGGG               
HincII         58  GTCGAC               GT[CT]^[GA]AC
             2134  GTCAAC              
HindII         58  GTCGAC               GT[CT]^[GA]AC
             2134  GTCAAC              
MaeII        1637  ACGT                 A^CGT
             2138  ACGT                
MroI         1586  TCCGGA               T^CCGGA
             1874  TCCGGA              
MslI         1935  CATCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2037  CATGAGCATG          
MvnI          962  CGCG                 CG^CG
             1309  CGCG                
NspI         2042  GCATGC               [GA]CATG^[CT]
             2253  GCATGC              
PvuII         625  CAGCTG               CAG^CTG
             2310  CAGCTG              
SphI         2042  GCATGC               GCATG^C
             2253  GCATGC              
TaqI           59  TCGA                 T^CGA
              879  TCGA                
TthHB8I        59  TCGA                 T^CGA
              879  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        AspHI       AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TfiI        TseI        Tsp45I      TspRI       
XcmI        XhoII       
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