Restriction map for pF1KB4980
 (Circular) (Six-base) MAPSORT of: pF1KB4980.seq  Check: 3366  from: 1  to: 5654



pF1KB4980  5654 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    191    2282     191 
   Size:       2091    3563
 
AccI GT'mk_AC

Cuts at:    167    2692     167 
   Size:       2525    3129
 
Acc65I G'GTAC_C

Cuts at:   2670    2670 
   Size:       5654
 
AclI AA'CG_TT

Cuts at:   5494    5494 
   Size:       5654
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    283     406     721     976    1498    1779    3479    3911    4264 
   Size:        123     315     255     522     281    1700     432     353

Cuts at:   4264    4926     283 
   Size:        662    1011

  Fragments arranged by size: 

                1700    1011     662     522     432     353     315     281
                 255     123
 
AflIII A'CryG_T

Cuts at:   2246    3006    4806    5087    2246 
   Size:        760    1800     281    2813

  Fragments arranged by size: 

                2813    1800     760     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    355     355 
   Size:       5654
 
AlwNI CAG_nnn'CTG

Cuts at:    286     735     954    1323    2337    2959    4397     286 
   Size:        449     219     369    1014     622    1438    1543

  Fragments arranged by size: 

                1543    1438    1014     622     449     369     219
 
ApaI G_GGCC'C

Cuts at:   1020    1020 
   Size:       5654
 
ApaLI G'TGCA_C

Cuts at:   4492    4492 
   Size:       5654
 
ApoI r'AATT_y

Cuts at:   2658    5007    5263    2658 
   Size:       2349     256    3049

  Fragments arranged by size: 

                3049    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5654
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5654
 
AvaI C'yCGr_G

Cuts at:    866    2674     866 
   Size:       1808    3846
 
BamHI G'GATC_C

Cuts at:    707    2679     707 
   Size:       1972    3682
 
BanI G'GyrC_C

Cuts at:   1443    2106    2172    2670    3084    3332    3367    1443 
   Size:        663      66     498     414     248      35    3730

  Fragments arranged by size: 

                3730     663     498     414     248      66      35
 
BanII G_rGCy'C

Cuts at:    860    1020    1772    2090    2668    3698     860 
   Size:        160     752     318     578    1030    2816

  Fragments arranged by size: 

                2816    1030     752     578     318     160
 
BbeI G_GCGC'C

Cuts at:   3088    3336    3088 
   Size:        248    5406
 
BbsI GAAGACnn'nnnn_

Cuts at:    964    2420    2477     964 
   Size:       1456      57    4141

  Fragments arranged by size: 

                4141    1456      57
 
BbvCI CC'TCA_GC

Cuts at:    572    1490     572 
   Size:        918    4736
 
BciVI GTATCCnnnnn_n'

Cuts at:   3552    4608    5654    3552 
   Size:       1056    1046    3552

  Fragments arranged by size: 

                3552    1056    1046
 
BclI T'GATC_A

Cuts at:   1718    3174    1718 
   Size:       1456    4198
 
BglI GCCn_nnn'nGGC

Cuts at:   1850    1850 
   Size:       5654
 
BglII A'GATC_T

Cuts at:   1547    3169    5206    1547 
   Size:       1622    2037    1995

  Fragments arranged by size: 

                2037    1995    1622
 
BlpI GC'TnA_GC

Cuts at:   1436    1937    2766    1436 
   Size:        501     829    4324

  Fragments arranged by size: 

                4324     829     501
 
Bme1580I G_kGCm'C

Cuts at:    273    1020    1145    1234    1424    1448    2177    2458    2568 
   Size:        747     125      89     190      24     729     281     110

Cuts at:   2568    3279    3372    4496     273 
   Size:        711      93    1124    1431

  Fragments arranged by size: 

                1431    1124     747     729     711     281     190     125
                 110      93      89      24
 
BmrI ACTGGGnnnn_n'

Cuts at:    635    1921    2636    2866    2947    3282    5426     635 
   Size:       1286     715     230      81     335    2144     863

  Fragments arranged by size: 

                2144    1286     863     715     335     230      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1103    4021    1103 
   Size:       2918    2736
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    229     689     763    1001    1124    1139    1300    2074    2548 
   Size:        460      74     238     123      15     161     774     474

Cuts at:   2548     229 
   Size:       3335

  Fragments arranged by size: 

                3335     774     474     460     238     161     123      74
                  15
 
Bpu10I CC'TnA_GC

Cuts at:    572    1490    2425     572 
   Size:        918     935    3801

  Fragments arranged by size: 

                3801     935     918
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    379     475     874    1023    1186    2830    3709    4423    4721 
   Size:         96     399     149     163    1644     879     714     298

Cuts at:   4721     379 
   Size:       1312

  Fragments arranged by size: 

                1644    1312     879     714     399     298     163     149
                  96
 
BsaI GGTCTCn'nnnn_

Cuts at:    132     655    5358     132 
   Size:        523    4703     428

  Fragments arranged by size: 

                4703     523     428
 
BsaAI yAC'GTr

Cuts at:   3007    3007 
   Size:       5654
 
BsaBI GATnn'nnATC

Cuts at:      6    3168    5205       6 
   Size:       3162    2037     455

  Fragments arranged by size: 

                3162    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    245    3085    3333    4035    5531     245 
   Size:       2840     248     702    1496     368

  Fragments arranged by size: 

                2840    1496     702     368     248
 
BsaWI w'CCGG_w

Cuts at:   1694    1982    2843    3364    4042    4453    4600    4874    1694 
   Size:        288     861     521     678     411     147     274    2474

  Fragments arranged by size: 

                2474     861     678     521     411     288     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1444    1474    1676    1706    2185    2215    2558    2588    1444 
   Size:         30     202      30     479      30     343      30    4510

  Fragments arranged by size: 

                4510     479     343     202      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    775    1264    1777    2474     775 
   Size:        489     513     697    3955

  Fragments arranged by size: 

                3955     697     513     489
 
BseYI C'CCAG_C

Cuts at:    815    1020    1629    2034    2287    3079    3837    4502    4910 
   Size:        205     609     405     253     792     758     665     408

Cuts at:   4910     815 
   Size:       1559

  Fragments arranged by size: 

                1559     792     758     665     609     408     405     253
                 205
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    172     577     172 
   Size:        405    5249
 
BsiEI CG_ry'CG

Cuts at:    104    3196    3242    4472     104 
   Size:       3092      46    1230    1286

  Fragments arranged by size: 

                3092    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    334    1268    1526    2090    2668    3446    3636    4496     334 
   Size:        934     258     564     578     778     190     860    1492

  Fragments arranged by size: 

                1492     934     860     778     578     564     258     190
            
 
BsmI GAATG_Cn'

Cuts at:   1157    1157 
   Size:       5654
 
Bsp1286I G_dGCh'C

Cuts at:    273     334     860    1020    1145    1234    1268    1424    1448 
   Size:         61     526     160     125      89      34     156      24

Cuts at:   1448    1526    1772    2090    2177    2458    2568    2668    3279 
   Size:         78     246     318      87     281     110     100     611

Cuts at:   3279    3372    3446    3636    3698    4496     273 
   Size:         93      74     190      62     798    1431

  Fragments arranged by size: 

                1431     798     611     526     318     281     246     190
                 160     156     125     110     100      93      89      87
                  78      74      62      61      34      24
 
BspEI T'CCGG_A

Cuts at:   1694    1982    1694 
   Size:        288    5366
 
BspHI T'CATG_A

Cuts at:   4086    4086 
   Size:       5654
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    191    1202    2282    2704    2918    3220    3601     191 
   Size:       1011    1080     422     214     302     381    2244

  Fragments arranged by size: 

                2244    1080    1011     422     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1224    1440    3946    4000    5484    5649      50 
   Size:       1174     216    2506      54    1484     165      55

  Fragments arranged by size: 

                2506    1484    1174     216     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1823    3566    1823 
   Size:       1743    3911
 
BsrFI r'CCGG_y

Cuts at:    884    1712    3652    3833     884 
   Size:        828    1940     181    2705

  Fragments arranged by size: 

                2705    1940     828     181
 
BssSI C'ACGA_G

Cuts at:   3925    4633    3925 
   Size:        708    4946
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3031    3031 
   Size:       5654
 
BstBI TT'CG_AA

Cuts at:   4015    4015 
   Size:       5654
 
BstYI r'GATC_y

Cuts at:      1     536     707    1547    1622    1929    2606    2679    3146 
   Size:        535     171     840      75     307     677      73     467

Cuts at:   3146    3169    3504    3750    4056    4068    4154    5206       1 
   Size:         23     335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052     840     677     535     467     449     335     307
                 306     246     171      86      75      73      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:   1088    2354    2450    2629    1088 
   Size:       1266      96     179    4113

  Fragments arranged by size: 

                4113    1266     179      96
 
BtgI C'CryG_G

Cuts at:   1350    1635    2054    2102    2405    1350 
   Size:        285     419      48     303    4599

  Fragments arranged by size: 

                4599     419     303     285      48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    857    3604    3785    3948     857 
   Size:       2747     181     163    2563

  Fragments arranged by size: 

                2747    2563     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1660    2897    1660 
   Size:       1237    4417
 
DraI TTT'AAA

Cuts at:   2653    2653 
   Size:       5654
 
DrdI GACnn_nn'nnGTC

Cuts at:   3360    4704    3360 
   Size:       1344    4310
 
EaeI y'GGCC_r

Cuts at:    395     579     644     882    3239    3413    3804    3831    5176 
   Size:        184      65     238    2357     174     391      27    1345

Cuts at:   5176     395 
   Size:        873

  Fragments arranged by size: 

                2357    1345     873     391     238     184     174      65
                  27
 
EagI C'GGCC_G

Cuts at:   3239    3239 
   Size:       5654
 
EarI CTCTTCn'nnn_

Cuts at:    697    1273    1537    3677    3887     697 
   Size:        576     264    2140     210    2464

  Fragments arranged by size: 

                2464    2140     576     264     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    439    3862    4604    4750    5464     439 
   Size:       3423     742     146     714     629

  Fragments arranged by size: 

                3423     742     714     629     146
 
EcoICRI GAG'CTC

Cuts at:   2088    2666    2088 
   Size:        578    5076
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    229     283     406     689     721     763     976    1001    1124 
   Size:         54     123     283      32      42     213      25     123

Cuts at:   1124    1139    1300    1498    1779    2074    2548    3479    3911 
   Size:         15     161     198     281     295     474     931     432

Cuts at:   3911    4264    4926     229 
   Size:        353     662     957

  Fragments arranged by size: 

                 957     931     662     474     432     353     295     283
                 281     213     198     161     123     123      54      42
                  32      25      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:    562     562 
   Size:       5654
 
EcoO109I rG'GnC_Cy

Cuts at:    378     639    1016    1017    1093    1320    2334    2508    2555 
   Size:        261     377       1      76     227    1014     174      47

Cuts at:   2555    2793     378 
   Size:        238    3239

  Fragments arranged by size: 

                3239    1014     377     261     238     227     174      76
                  47       1
 
EcoRI G'AATT_C

Cuts at:   2658    2658 
   Size:       5654
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    522    3673     522 
   Size:       3151    2503
 
FspI TGC'GCA

Cuts at:   3435    3435 
   Size:       5654
 
HaeII r_GCGC'y

Cuts at:   3088    3336    4566    5337    3088 
   Size:        248    1230     771    3405

  Fragments arranged by size: 

                3405    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    354     386     594     626    1112    1144    3496    3526    3528 
   Size:         32     208      32     486      32    2352      30       2

Cuts at:   3528    3558     354 
   Size:         30    2450

  Fragments arranged by size: 

                2450    2352     486     208      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    168    2244    2693     168 
   Size:       2076     449    3129

  Fragments arranged by size: 

                3129    2076     449
 
HindIII A'AGCT_T

Cuts at:   2518    2518 
   Size:       5654
 
KasI G'GCGC_C

Cuts at:   3084    3332    3084 
   Size:        248    5406
 
KpnI G_GTAC'C

Cuts at:   2674    2674 
   Size:       5654
 
MluI A'CGCG_T

Cuts at:   5087    5087 
   Size:       5654
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    266    4413    4597     266 
   Size:       4147     184    1323

  Fragments arranged by size: 

                4147    1323     184
 
MscI TGG'CCA

Cuts at:    397     581     646    3415     397 
   Size:        184      65    2769    2636

  Fragments arranged by size: 

                2769    2636     184      65
 
MslI CAynn'nnrTG

Cuts at:   2047    2149    3643    3770    2047 
   Size:        102    1494     127    3931

  Fragments arranged by size: 

                3931    1494     127     102
 
MspA1I CmG'CkG

Cuts at:    156     735    1035    2420    2765    3079    3439    4223    4468 
   Size:        579     300    1385     345     314     360     784     245

Cuts at:   4468     156 
   Size:       1342

  Fragments arranged by size: 

                1385    1342     784     579     360     345     314     300
                 245
 
NaeI GCC'GGC

Cuts at:    886    3835     886 
   Size:       2949    2705
 
NarI GG'CG_CC

Cuts at:   3085    3333    3085 
   Size:        248    5406
 
NcoI C'CATG_G

Cuts at:   1350    2102    2405    1350 
   Size:        752     303    4599

  Fragments arranged by size: 

                4599     752     303
 
NgoMIV G'CCGG_C

Cuts at:    884    3833     884 
   Size:       2949    2705
 
NspI r_CATG'y

Cuts at:   2154    2365    2707    3207    4810    2154 
   Size:        211     342     500    1603    2998

  Fragments arranged by size: 

                2998    1603     500     342     211
 
PasI CC'CwG_GG

Cuts at:    221    1448    2331     221 
   Size:       1227     883    3544

  Fragments arranged by size: 

                3544    1227     883
 
PciI A'CATG_T

Cuts at:   4806    4806 
   Size:       5654
 
PfoI T'CCnGG_A

Cuts at:    779    1670    1925    2261    2525     779 
   Size:        891     255     336     264    3908

  Fragments arranged by size: 

                3908     891     336     264     255
 
PmeI GTTT'AAAC

Cuts at:   2653    2653 
   Size:       5654
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1159    1191    2495    2527    4100    4132    1159 
   Size:         32    1304      32    1573      32    2681

  Fragments arranged by size: 

                2681    1573    1304      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    378    1093    2508    2555     378 
   Size:        715    1415      47    3477

  Fragments arranged by size: 

                3477    1415     715      47
 
PspOMI G'GGCC_C

Cuts at:   1016    1016 
   Size:       5654
 
PstI C_TGCA'G

Cuts at:    521     563     659    2279    2582    2701    3386     521 
   Size:         42      96    1620     303     119     685    2789

  Fragments arranged by size: 

                2789    1620     685     303     119      96      42
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5654
 
PvuII CAG'CTG

Cuts at:    735    2420    3079    3439     735 
   Size:       1685     659     360    2950

  Fragments arranged by size: 

                2950    1685     659     360
 
RsrII CG'GwC_CG

Cuts at:   3849    3849 
   Size:       5654
 
SacI G_AGCT'C

Cuts at:   2090    2668    2090 
   Size:        578    5076
 
SalI G'TCGA_C

Cuts at:    166    2691     166 
   Size:       2525    3129
 
SanDI GG'GwC_CC

Cuts at:    378     378 
   Size:       5654
 
SapI GCTCTTCn'nnn_

Cuts at:   1273    3677    3887    1273 
   Size:       2404     210    3040

  Fragments arranged by size: 

                3040    2404     210
 
SbfI CC_TGCA'GG

Cuts at:   2701    2701 
   Size:       5654
 
ScaI AGT'ACT

Cuts at:   5171    5171 
   Size:       5654
 
SfcI C'TryA_G

Cuts at:     33     517     559     655     820    2275    2578    2697    3382 
   Size:        484      42      96     165    1455     303     119     685

Cuts at:   3382    4350    4541      33 
   Size:        968     191    1146

  Fragments arranged by size: 

                1455    1146     968     685     484     303     191     165
                 119      96      42
 
SfoI GGC'GCC

Cuts at:   3086    3334    3086 
   Size:        248    5406
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5654
 
SmaI CCC'GGG

Cuts at:    868    2676     868 
   Size:       1808    3846
 
SmlI C'TyrA_G

Cuts at:    358     490     853    1002    1201    2809    3724    4438    4700 
   Size:        132     363     149     199    1608     915     714     262

Cuts at:   4700     358 
   Size:       1312

  Fragments arranged by size: 

                1608    1312     915     714     363     262     199     149
                 132
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5654
 
SphI G_CATG'C

Cuts at:   2154    2365    2707    3207    2154 
   Size:        211     342     500    4601

  Fragments arranged by size: 

                4601     500     342     211
 
StuI AGG'CCT

Cuts at:    304    1579    2222     304 
   Size:       1275     643    3736

  Fragments arranged by size: 

                3736    1275     643
 
StyI C'CwwG_G

Cuts at:   1145    1350    1967    2102    2121    2405    2558    2788    3142 
   Size:        205     617     135      19     284     153     230     354

Cuts at:   3142    1145 
   Size:       3657

  Fragments arranged by size: 

                3657     617     354     284     230     205     153     135
                  19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    187    1934    3237    3370    3829    4038     187 
   Size:       1747    1303     133     459     209    1803

  Fragments arranged by size: 

                1803    1747    1303     459     209     133
 
TatI w'GTAC_w

Cuts at:   5169    5169 
   Size:       5654
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1444    1476    2185    2217    2558    2590    5171    5203    1444 
   Size:         32     709      32     341      32    2581      32    1895

  Fragments arranged by size: 

                2581    1895     709     341      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:    286    3451     286 
   Size:       3165    2489
 
XbaI T'CTAG_A

Cuts at:   2685    2685 
   Size:       5654
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    924    1032    1908    3149     924 
   Size:        108     876    1241    3429

  Fragments arranged by size: 

                3429    1241     876     108
 
XmaI C'CCGG_G

Cuts at:    866    2674     866 
   Size:       1808    3846
 
XmnI GAAnn'nnTTC

Cuts at:   1549    1549 
   Size:       5654
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AleI     AloI     AscI
    AvrII     BaeI     BcgI    BfrBI    BmgBI     BmtI    BsiWI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII
    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PmlI    PshAI     PsiI    PspXI
     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     ZraI
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