Restriction map for pF1KB4980
 (Linear) (Six-base) MAPSORT of: KIBB4980.seq  Check: 7086  from: 1  to: 2541



KIBB4980.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      83    2174    2541 
   Size:         83    2091     367

  Fragments arranged by size: 

                2091     367      83
 
AccI GT'mk_AC

Cuts at:      0      59    2541 
   Size:         59    2482
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     298     613     868    1390    1671    2541 
   Size:        175     123     315     255     522     281     870

  Fragments arranged by size: 

                 870     522     315     281     255     175     123
 
AflIII A'CryG_T

Cuts at:      0    2138    2541 
   Size:       2138     403
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     247    2541 
   Size:        247    2294
 
AlwNI CAG_nnn'CTG

Cuts at:      0     178     627     846    1215    2229    2541 
   Size:        178     449     219     369    1014     312

  Fragments arranged by size: 

                1014     449     369     312     219     178
 
ApaI G_GGCC'C

Cuts at:      0     912    2541 
   Size:        912    1629
 
AvaI C'yCGr_G

Cuts at:      0     758    2541 
   Size:        758    1783
 
BamHI G'GATC_C

Cuts at:      0     599    2541 
   Size:        599    1942
 
BanI G'GyrC_C

Cuts at:      0    1335    1998    2064    2541 
   Size:       1335     663      66     477

  Fragments arranged by size: 

                1335     663     477      66
 
BanII G_rGCy'C

Cuts at:      0     752     912    1664    1982    2541 
   Size:        752     160     752     318     559

  Fragments arranged by size: 

                 752     752     559     318     160
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     856    2312    2369    2541 
   Size:        856    1456      57     172

  Fragments arranged by size: 

                1456     856     172      57
 
BbvCI CC'TCA_GC

Cuts at:      0     464    1382    2541 
   Size:        464     918    1159

  Fragments arranged by size: 

                1159     918     464
 
BclI T'GATC_A

Cuts at:      0    1610    2541 
   Size:       1610     931
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1742    2541 
   Size:       1742     799
 
BglII A'GATC_T

Cuts at:      0    1439    2541 
   Size:       1439    1102
 
BlpI GC'TnA_GC

Cuts at:      0    1328    1829    2541 
   Size:       1328     501     712

  Fragments arranged by size: 

                1328     712     501
 
Bme1580I G_kGCm'C

Cuts at:      0     165     912    1037    1126    1316    1340    2069    2350 
   Size:        165     747     125      89     190      24     729     281

Cuts at:   2350    2460    2541 
   Size:        110      81

  Fragments arranged by size: 

                 747     729     281     190     165     125     110      89
                  81      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527    1813    2528    2541 
   Size:        527    1286     715      13

  Fragments arranged by size: 

                1286     715     527      13
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     995    2541 
   Size:        995    1546
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     581     655     893    1016    1031    1192    1966 
   Size:        121     460      74     238     123      15     161     774

Cuts at:   1966    2440    2541 
   Size:        474     101

  Fragments arranged by size: 

                 774     474     460     238     161     123     121     101
                  74      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     464    1382    2317    2541 
   Size:        464     918     935     224

  Fragments arranged by size: 

                 935     918     464     224
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     367     766     915    1078    2541 
   Size:        271      96     399     149     163    1463

  Fragments arranged by size: 

                1463     399     271     163     149      96
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      24     547    2541 
   Size:         24     523    1994

  Fragments arranged by size: 

                1994     523      24
 
BsaHI Gr'CG_yC

Cuts at:      0     137    2541 
   Size:        137    2404
 
BsaWI w'CCGG_w

Cuts at:      0    1586    1874    2541 
   Size:       1586     288     667

  Fragments arranged by size: 

                1586     667     288
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1336    1366    1568    1598    2077    2107    2450    2480 
   Size:       1336      30     202      30     479      30     343      30

Cuts at:   2480    2541 
   Size:         61

  Fragments arranged by size: 

                1336     479     343     202      61      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     667    1156    1669    2366    2541 
   Size:        667     489     513     697     175

  Fragments arranged by size: 

                 697     667     513     489     175
 
BseYI C'CCAG_C

Cuts at:      0     707     912    1521    1926    2179    2541 
   Size:        707     205     609     405     253     362

  Fragments arranged by size: 

                 707     609     405     362     253     205
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     469    2541 
   Size:         64     405    2072

  Fragments arranged by size: 

                2072     405      64
 
BsiHKAI G_wGCw'C

Cuts at:      0     226    1160    1418    1982    2541 
   Size:        226     934     258     564     559

  Fragments arranged by size: 

                 934     564     559     258     226
 
BsmI GAATG_Cn'

Cuts at:      0    1049    2541 
   Size:       1049    1492
 
Bsp1286I G_dGCh'C

Cuts at:      0     165     226     752     912    1037    1126    1160    1316 
   Size:        165      61     526     160     125      89      34     156

Cuts at:   1316    1340    1418    1664    1982    2069    2350    2460    2541 
   Size:         24      78     246     318      87     281     110      81

  Fragments arranged by size: 

                 526     318     281     246     165     160     156     125
                 110      89      87      81      78      61      34      24
            
 
BspEI T'CCGG_A

Cuts at:      0    1586    1874    2541 
   Size:       1586     288     667

  Fragments arranged by size: 

                1586     667     288
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83    1094    2174    2541 
   Size:         83    1011    1080     367

  Fragments arranged by size: 

                1080    1011     367      83
 
BsrBI CCG'CTC

Cuts at:      0    1116    1332    2541 
   Size:       1116     216    1209

  Fragments arranged by size: 

                1209    1116     216
 
BsrDI GCAATG_nn'

Cuts at:      0    1715    2541 
   Size:       1715     826
 
BsrFI r'CCGG_y

Cuts at:      0     776    1604    2541 
   Size:        776     828     937

  Fragments arranged by size: 

                 937     828     776
 
BstYI r'GATC_y

Cuts at:      0     428     599    1439    1514    1821    2498    2541 
   Size:        428     171     840      75     307     677      43

  Fragments arranged by size: 

                 840     677     428     307     171      75      43
 
Bsu36I CC'TnA_GG

Cuts at:      0     980    2246    2342    2521    2541 
   Size:        980    1266      96     179      20

  Fragments arranged by size: 

                1266     980     179      96      20
 
BtgI C'CryG_G

Cuts at:      0    1242    1527    1946    1994    2297    2541 
   Size:       1242     285     419      48     303     244

  Fragments arranged by size: 

                1242     419     303     285     244      48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     749    2541 
   Size:        749    1792
 
BtsI GCAGTG_nn'

Cuts at:      0    1552    2541 
   Size:       1552     989
 
EaeI y'GGCC_r

Cuts at:      0     287     471     536     774    2541 
   Size:        287     184      65     238    1767

  Fragments arranged by size: 

                1767     287     238     184      65
 
EarI CTCTTCn'nnn_

Cuts at:      0     589    1165    1429    2541 
   Size:        589     576     264    1112

  Fragments arranged by size: 

                1112     589     576     264
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     331    2541 
   Size:        331    2210
 
EcoICRI GAG'CTC

Cuts at:      0    1980    2541 
   Size:       1980     561
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     175     298     581     613     655     868     893 
   Size:        121      54     123     283      32      42     213      25

Cuts at:    893    1016    1031    1192    1390    1671    1966    2440    2541 
   Size:        123      15     161     198     281     295     474     101

  Fragments arranged by size: 

                 474     295     283     281     213     198     161     123
                 123     121     101      54      42      32      25      15
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     454    2541 
   Size:        454    2087
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270     531     908     909     985    1212    2226    2400 
   Size:        270     261     377       1      76     227    1014     174

Cuts at:   2400    2447    2541 
   Size:         47      94

  Fragments arranged by size: 

                1014     377     270     261     227     174      94      76
                  47       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     414    2541 
   Size:        414    2127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278     486     518    1004    1036    2541 
   Size:        246      32     208      32     486      32    1505

  Fragments arranged by size: 

                1505     486     246     208      32      32      32
 
HincII GTy'rAC

Cuts at:      0      60    2136    2541 
   Size:         60    2076     405

  Fragments arranged by size: 

                2076     405      60
 
HindIII A'AGCT_T

Cuts at:      0    2410    2541 
   Size:       2410     131
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    2541 
   Size:        158    2383
 
MscI TGG'CCA

Cuts at:      0     289     473     538    2541 
   Size:        289     184      65    2003

  Fragments arranged by size: 

                2003     289     184      65
 
MslI CAynn'nnrTG

Cuts at:      0    1939    2041    2541 
   Size:       1939     102     500

  Fragments arranged by size: 

                1939     500     102
 
MspA1I CmG'CkG

Cuts at:      0      48     627     927    2312    2541 
   Size:         48     579     300    1385     229

  Fragments arranged by size: 

                1385     579     300     229      48
 
NaeI GCC'GGC

Cuts at:      0     778    2541 
   Size:        778    1763
 
NcoI C'CATG_G

Cuts at:      0    1242    1994    2297    2541 
   Size:       1242     752     303     244

  Fragments arranged by size: 

                1242     752     303     244
 
NgoMIV G'CCGG_C

Cuts at:      0     776    2541 
   Size:        776    1765
 
NspI r_CATG'y

Cuts at:      0    2046    2257    2541 
   Size:       2046     211     284

  Fragments arranged by size: 

                2046     284     211
 
PasI CC'CwG_GG

Cuts at:      0     113    1340    2223    2541 
   Size:        113    1227     883     318

  Fragments arranged by size: 

                1227     883     318     113
 
PfoI T'CCnGG_A

Cuts at:      0     671    1562    1817    2153    2417    2541 
   Size:        671     891     255     336     264     124

  Fragments arranged by size: 

                 891     671     336     264     255     124
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1051    1083    2387    2419    2541 
   Size:       1051      32    1304      32     122

  Fragments arranged by size: 

                1304    1051     122      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     270     985    2400    2447    2541 
   Size:        270     715    1415      47      94

  Fragments arranged by size: 

                1415     715     270      94      47
 
PspOMI G'GGCC_C

Cuts at:      0     908    2541 
   Size:        908    1633
 
PstI C_TGCA'G

Cuts at:      0     413     455     551    2171    2474    2541 
   Size:        413      42      96    1620     303      67

  Fragments arranged by size: 

                1620     413     303      96      67      42
 
PvuII CAG'CTG

Cuts at:      0     627    2312    2541 
   Size:        627    1685     229

  Fragments arranged by size: 

                1685     627     229
 
SacI G_AGCT'C

Cuts at:      0    1982    2541 
   Size:       1982     559
 
SalI G'TCGA_C

Cuts at:      0      58    2541 
   Size:         58    2483
 
SanDI GG'GwC_CC

Cuts at:      0     270    2541 
   Size:        270    2271
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1165    2541 
   Size:       1165    1376
 
SfcI C'TryA_G

Cuts at:      0     409     451     547     712    2167    2470    2541 
   Size:        409      42      96     165    1455     303      71

  Fragments arranged by size: 

                1455     409     303     165      96      71      42
 
SmaI CCC'GGG

Cuts at:      0     760    2541 
   Size:        760    1781
 
SmlI C'TyrA_G

Cuts at:      0     250     382     745     894    1093    2541 
   Size:        250     132     363     149     199    1448

  Fragments arranged by size: 

                1448     363     250     199     149     132
 
SphI G_CATG'C

Cuts at:      0    2046    2257    2541 
   Size:       2046     211     284

  Fragments arranged by size: 

                2046     284     211
 
StuI AGG'CCT

Cuts at:      0     196    1471    2114    2541 
   Size:        196    1275     643     427

  Fragments arranged by size: 

                1275     643     427     196
 
StyI C'CwwG_G

Cuts at:      0    1037    1242    1859    1994    2013    2297    2450    2541 
   Size:       1037     205     617     135      19     284     153      91

  Fragments arranged by size: 

                1037     617     284     205     153     135      91      19
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79    1826    2541 
   Size:         79    1747     715

  Fragments arranged by size: 

                1747     715      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1336    1368    2077    2109    2450    2482    2541 
   Size:       1336      32     709      32     341      32      59

  Fragments arranged by size: 

                1336     709     341      59      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     178    2541 
   Size:        178    2363
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     816     924    1800    2541 
   Size:        816     108     876     741

  Fragments arranged by size: 

                 876     816     741     108
 
XmaI C'CCGG_G

Cuts at:      0     758    2541 
   Size:        758    1783
 
XmnI GAAnn'nnTTC

Cuts at:      0    1441    2541 
   Size:       1441    1100
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AlfI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbsI    BbvCI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI     PfoI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI     SalI    SanDI
     SapI     SfcI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI     BcgI    BciVI    BfrBI    BmgBI     BmtI    BsaAI    BsaBI
    BsiEI    BsiWI    BsmBI    BspHI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     TatI     XbaI     XhoI     ZraI
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