Restriction map for pF1KB4964
##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

Acc65I      AccIII      AflII       Aor51HI     AscI        
AseI        AsnI        Asp718I     AspI        AviII       
BbvCI       BfrI        BsaBI       BseAI       BsgI        
BsiWI       BspEI       BsrDI       BssHII      Bst1107I    
BstZ17I     EagI        EclXI       Eco47III    Eco52I      
EcoRV       FseI        FspI        KpnI        KspI        
MamI        MroI        NdeI        NotI        NruI        
PacI        PaeR7I      PflFI       PmeI        PshBI       
PvuI        SacII       SbfI        ScaI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII         420  GACGTC               GACGT^C
AclI         1550  AACGTT               AA^CGTT
AgeI         2275  ACCGGT               A^CCGGT
AocI         1152  CCTGAGG              CC^T[ACGT]AGG
ApaI          165  GGGCCC               GGGCC^C
Asp700I      3949  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         2900  TGGCCA               TGG^CCA
BbeI           63  GGCGCC               GGCGC^C
BcgI         1522  GCAGTTGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1747  GGATAC               GTATCC(6/5)
BglII        3347  AGATCT               A^GATCT
BlpI         1146  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1146  GCTGAGC              GC^T[ACGT]AGC
Bsp1407I     4358  TGTACA               T^GTACA
BspDI        3742  ATCGAT               AT^CGAT
BspLU11I     2678  ACATGT               A^CATGT
BspMI        3792  GCAGGT               ACCTGC(4/8)
BsrBI         729  CCGCTC               CCGCTC(-3/-3)
BsrGI        4358  TGTACA               T^GTACA
BssSI         292  CTCGTG               CACGAG(-5/-1)
BstBI         152  TTCGAA               TT^CGAA
BstXI        1076  CCAGGCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1152  CCTGAGG              CC^T[ACGT]AGG
CelII        1146  GCTGAGC              GC^T[ACGT]AGC
ClaI         3742  ATCGAT               AT^CGAT
CpoI           57  CGGTCCG              CG^G[AT]CCG
Eco81I       1152  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1230  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         544  GAATTC               G^AATTC
EcoT22I      2101  ATGCAT               ATGCA^T
HindIII      1396  AAGCTT               A^AGCTT
HpaI         1465  GTTAAC               GTT^AAC
KasI           63  GGCGCC               G^GCGCC
MfeI         1799  CAATTG               C^AATTG
MluI         4353  ACGCGT               A^CGCGT
MluNI        2900  TGGCCA               TGG^CCA
MscI         2900  TGGCCA               TGG^CCA
MunI         1799  CAATTG               C^AATTG
NaeI           36  GCCGGC               GCC^GGC
NarI           63  GGCGCC               GG^CGCC
NgoMIV         36  GCCGGC               G^CCGGC
NheI         1927  GCTAGC               G^CTAGC
NsiI         2101  ATGCAT               ATGCA^T
NspV          152  TTCGAA               TT^CGAA
PinAI        2275  ACCGGT               A^CCGGT
PshAI         642  GACACAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1550  AACGTT               AA^CGTT
RsrII          57  CGGTCCG              CG^G[AT]CCG
SacI          796  GAGCTC               GAGCT^C
SalI         3886  GTCGAC               G^TCGAC
SapI         2577  GCTCTTC              GCTCTTC(1/4)
SexAI        1064  ACCAGGT              A^CC[AT]GGT
SfoI           63  GGCGCC               GGC^GCC
SfuI          152  TTCGAA               TT^CGAA
SspBI        4358  TGTACA               T^GTACA
SspI          103  AATATT               AAT^ATT
XcmI         1877  CCAGCAAGATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         3949  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AhdI          262  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2833  GACAACAGGTC         
Alw44I       1069  GTGCAC               G^TGCAC
             2620  GTGCAC              
AlwNI         331  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3642  CAGAAGCTG           
ApaLI        1069  GTGCAC               G^TGCAC
             2620  GTGCAC              
AspEI         262  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2833  GACAACAGGTC         
AvrII        3145  CCTAGG               C^CTAGG
             3895  CCTAGG              
BanI           63  GGCGCC               G^G[CT][GA]CC
             1624  GGCACC              
BbrPI         253  CACGTG               CAC^GTG
             3204  CACGTG              
BclI          398  TGATCA               T^GATCA
              608  TGATCA              
BglI         1939  GCCAATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2476  GCCGCCATGGC         
BlnI         3145  CCTAGG               C^CTAGG
             3895  CCTAGG              
BsaAI         253  CACGTG               [CT]AC^GT[GA]
             3204  CACGTG              
BsiEI        2562  CGACCG               CG[GA][CT]^CG
             4204  CGGTCG              
BsmBI        1629  CGTCTC               CGTCTC(1/5)
             1279  GAGACG              
BspHI        1955  TCATGA               T^CATGA
             2019  TCATGA              
DraI         3256  TTTAAA               TTT^AAA
             4332  TTTAAA              
DraIII        130  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1159  CACGCCGTG           
DrdI          496  GACTGGCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2143  GACGCCTTGGTC        
EaeI         2900  TGGCCA               [CT]^GGCC[GA]
             2993  TGGCCG              
Eam1105I      262  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2833  GACAACAGGTC         
FbaI          398  TGATCA               T^GATCA
              608  TGATCA              
HaeII          63  GGCGCC               [GA]GCGC^[CT]
             1660  AGCGCC              
NcoI         1782  CCATGG               C^CATGG
             2480  CCATGG              
PmaCI         253  CACGTG               CAC^GTG
             3204  CACGTG              
PmlI          253  CACGTG               CAC^GTG
             3204  CACGTG              
PstI         3639  CTGCAG               CTGCA^G
             4341  CTGCAG              
RcaI         1955  TCATGA               T^CATGA
             2019  TCATGA              
XbaI         1647  TCTAGA               T^CTAGA
             3022  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        ApoI        
AspHI       AvaI        AvaII       BamHI       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
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