Restriction map for pF1KB4944
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaI        BseRI       BsgI        BsiWI       BsmBI       
BspDI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MscI        MspA1I      MunI        MvaI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII       1382  TCCGGA               T^CCGGA
AcsI          787  GAATTT               [GA]^AATT[CT]
AcyI         1285  GGCGTC               G[GA]^CG[CT]C
AocI          201  CCTGAGG              CC^T[ACGT]AGG
ApoI          787  GAATTT               [GA]^AATT[CT]
AspI           34  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1343  CCCGGG               C^[CT]CG[GA]G
AviII        1500  TGCGCA               TGC^GCA
BanII         824  GAGCCC               G[GA]GC[CT]^C
BbvCI        1481  CCTCAGC              CCTCAGC(-5/-2)
BsaHI        1285  GGCGTC               G[GA]^CG[CT]C
BsaWI        1382  TCCGGA               [AT]^CCGG[AT]
BseAI        1382  TCCGGA               T^CCGGA
BsiEI          33  CGACCG               CG[GA][CT]^CG
BsmFI          52  GTCCC                GGGAC(10/14)
BsoBI        1343  CCCGGG               C^[CT]CG[GA]G
BspEI        1382  TCCGGA               T^CCGGA
BspMI         674  ACCTGC               ACCTGC(4/8)
BsrDI         707  GCAATG               GCAATG(2/0)
BstXI         837  CCAAGATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1379  AGATCC               [GA]^GATC[CT]
Bsu36I        201  CCTGAGG              CC^T[ACGT]AGG
DraII        1253  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eco81I        201  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1253  AGGGCCT              [GA]G^G[ACGT]CC[CT]
FauI          652  CCCGC                CCCGC(4/6)
FspI         1500  TGCGCA               TGC^GCA
HaeII          11  GGCGCT               [GA]GCGC^[CT]
Hin1I        1285  GGCGTC               G[GA]^CG[CT]C
MflI         1379  AGATCC               [GA]^GATC[CT]
MroI         1382  TCCGGA               T^CCGGA
NdeI         1098  CATATG               CA^TATG
NlaIV         645  GGAACC               GG[ACGT]^[ACGT]CC
NspI         1494  ACATGC               [GA]CATG^[CT]
PflFI          34  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          776  CTGCAG               CTGCA^G
SapI          864  GAAGAGC              GCTCTTC(1/4)
SmaI         1343  CCCGGG               CCC^GGG
SmlI         1249  CTCAAG               C^T[CT][GA]AG
SspI          494  AATATT               AAT^ATT
TfiI          488  GAATC                G^A[AT]TC
TspRI         741  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        34  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1379  AGATCC               [GA]^GATC[CT]
XmaCI        1343  CCCGGG               C^CCGGG
XmaI         1343  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          37  CGCG                 CG^CG
             1400  CGCG                
AfaI          580  GTAC                 GT^AC
              858  GTAC                
AluI           48  AGCT                 AG^CT
              942  AGCT                
AseI          400  ATTAAT               AT^TAAT
             1417  ATTAAT              
AsnI          400  ATTAAT               AT^TAAT
             1417  ATTAAT              
AvaII         661  GGACC                G^G[AT]CC
             1410  GGACC               
BfaI          311  CTAG                 C^TAG
              922  CTAG                
BsmAI         526  GAGAC                GTCTC(1/5)
             1315  GAGAC               
BsmI          442  GAATGC               GAATGC(1/-1)
             1427  GAATGC              
Bsp1407I      579  TGTACA               T^GTACA
              857  TGTACA              
BspHI         438  TCATGA               T^CATGA
              971  TCATGA              
BsrGI         579  TGTACA               T^GTACA
              857  TGTACA              
BsrI          153  ACTGG                ACTGG(1/-1)
             1010  ACTGG               
BstUI          37  CGCG                 CG^CG
             1400  CGCG                
Cac8I         446  GCAGGC               GC[ACGT]^[ACGT]GC
             1301  GCAAGC              
DraI          123  TTTAAA               TTT^AAA
              393  TTTAAA              
EarI          157  GAAGAG               CTCTTC(1/4)
              864  GAAGAG              
EcoT14I       719  CCTTGG               C^C[AT][AT]GG
             1515  CCAAGG              
HgaI           38  GCGTC                GACGC(5/10)
             1286  GCGTC               
Ksp632I       157  GAAGAG               CTCTTC(1/4)
              864  GAAGAG              
MaeI          311  CTAG                 C^TAG
              922  CTAG                
MvnI           37  CGCG                 CG^CG
             1400  CGCG                
PleI         1245  GACTC                GAGTC(4/5)
             1260  GACTC               
PshBI         400  ATTAAT               AT^TAAT
             1417  ATTAAT              
RcaI          438  TCATGA               T^CATGA
              971  TCATGA              
RsaI          580  GTAC                 GT^AC
              858  GTAC                
SspBI         579  TGTACA               T^GTACA
              857  TGTACA              
StyI          719  CCTTGG               C^C[AT][AT]GG
             1515  CCAAGG              
XcmI         1000  CCACACCCCTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1187  CCATTGGGAAGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AlwI        AspHI       BbsI        BbvI        
BcnI        BmyI        BpuAI       BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
CfoI        Cfr13I      DdeI        DpnII       Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MwoI        NciI        NdeII       NlaIII      
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TthHB8I     
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