Restriction map for pF1KB4944
 (Circular) (Six-base) MAPSORT of: pF1KB4944.seq  Check: 2608  from: 1  to: 4649



pF1KB4944  4649 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:   1687    1687 
   Size:       4649
 
Acc65I G'GTAC_C

Cuts at:   1665    1665 
   Size:       4649
 
AclI AA'CG_TT

Cuts at:   4489    4489 
   Size:       4649
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1462    2474    2906    3259    3921    1462 
   Size:       1012     432     353     662    2190

  Fragments arranged by size: 

                2190    1012     662     432     353
 
AflIII A'CryG_T

Cuts at:   2001    3801    4082    2001 
   Size:       1800     281    2568

  Fragments arranged by size: 

                2568    1800     281
 
AleI CACnn'nnGTG

Cuts at:   1565    1565 
   Size:       4649
 
AlwNI CAG_nnn'CTG

Cuts at:   1954    3392    1954 
   Size:       1438    3211
 
ApaLI G'TGCA_C

Cuts at:   3487    3487 
   Size:       4649
 
ApoI r'AATT_y

Cuts at:    895    1653    4002    4258     895 
   Size:        758    2349     256    1286

  Fragments arranged by size: 

                2349    1286     758     256
 
AseI AT'TA_AT

Cuts at:     20     509    1526      20 
   Size:        489    1017    3143

  Fragments arranged by size: 

                3143    1017     489
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4649
 
AvaI C'yCGr_G

Cuts at:   1451    1669    1451 
   Size:        218    4431
 
BamHI G'GATC_C

Cuts at:   1674    1674 
   Size:       4649
 
BanI G'GyrC_C

Cuts at:   1665    2079    2327    2362    1665 
   Size:        414     248      35    3952

  Fragments arranged by size: 

                3952     414     248      35
 
BanII G_rGCy'C

Cuts at:    936    1663    2693     936 
   Size:        727    1030    2892

  Fragments arranged by size: 

                2892    1030     727
 
BbeI G_GCGC'C

Cuts at:   2083    2331    2083 
   Size:        248    4401
 
BbsI GAAGACnn'nnnn_

Cuts at:    328     640     829     328 
   Size:        312     189    4148

  Fragments arranged by size: 

                4148     312     189
 
BbvCI CC'TCA_GC

Cuts at:   1590    1590 
   Size:       4649
 
BciVI GTATCCnnnnn_n'

Cuts at:   2547    3603    4649    2547 
   Size:       1056    1046    2547

  Fragments arranged by size: 

                2547    1056    1046
 
BclI T'GATC_A

Cuts at:   2169    2169 
   Size:       4649
 
BglII A'GATC_T

Cuts at:   2164    4201    2164 
   Size:       2037    2612
 
BlpI GC'TnA_GC

Cuts at:   1761    1761 
   Size:       4649
 
Bme1580I G_kGCm'C

Cuts at:   2274    2367    3491    2274 
   Size:         93    1124    3432

  Fragments arranged by size: 

                3432    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1861    1942    2277    4421    1861 
   Size:         81     335    2144    2089

  Fragments arranged by size: 

                2144    2089     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3016    3016 
   Size:       4649
 
Bpu10I CC'TnA_GC

Cuts at:   1590    1590 
   Size:       4649
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1342    1825    2704    3418    3716    1342 
   Size:        483     879     714     298    2275

  Fragments arranged by size: 

                2275     879     714     483     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4353    4353 
   Size:       4649
 
BsaAI yAC'GTr

Cuts at:   2002    2002 
   Size:       4649
 
BsaBI GATnn'nnATC

Cuts at:      6    2163    4200       6 
   Size:       2157    2037     455

  Fragments arranged by size: 

                2157    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1394    2080    2328    3030    4526    1394 
   Size:        686     248     702    1496    1517

  Fragments arranged by size: 

                1517    1496     702     686     248
 
BsaWI w'CCGG_w

Cuts at:   1490    1838    2359    3037    3448    3595    3869    1490 
   Size:        348     521     678     411     147     274    2270

  Fragments arranged by size: 

                2270     678     521     411     348     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    741     771     741 
   Size:         30    4619
 
BseYI C'CCAG_C

Cuts at:    952    2074    2832    3497    3905     952 
   Size:       1122     758     665     408    1696

  Fragments arranged by size: 

                1696    1122     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104     144    2191    2237    3467     104 
   Size:         40    2047      46    1230    1286

  Fragments arranged by size: 

                2047    1286    1230      46      40
 
BsiHKAI G_wGCw'C

Cuts at:    836     865    1202    1663    2441    2631    3491     836 
   Size:         29     337     461     778     190     860    1994

  Fragments arranged by size: 

                1994     860     778     461     337     190      29
 
BsmI GAATG_Cn'

Cuts at:    556    1541     556 
   Size:        985    3664
 
Bsp1286I G_dGCh'C

Cuts at:    836     865     936    1202    1663    2274    2367    2441    2631 
   Size:         29      71     266     461     611      93      74     190

Cuts at:   2631    2693    3491     836 
   Size:         62     798    1994

  Fragments arranged by size: 

                1994     798     611     461     266     190      93      74
                  71      62      29
 
BspEI T'CCGG_A

Cuts at:   1490    1490 
   Size:       4649
 
BspHI T'CATG_A

Cuts at:    546    1079    3081     546 
   Size:        533    2002    2114

  Fragments arranged by size: 

                2114    2002     533
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    791    1699    1913    2215    2596     791 
   Size:        908     214     302     381    2844

  Fragments arranged by size: 

                2844     908     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2941    2995    4479    4644      50 
   Size:       2891      54    1484     165      55

  Fragments arranged by size: 

                2891    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    822    2561     822 
   Size:       1739    2910
 
BsrFI r'CCGG_y

Cuts at:   2647    2828    2647 
   Size:        181    4468
 
BsrGI T'GTAC_A

Cuts at:    687     965     687 
   Size:        278    4371
 
BssSI C'ACGA_G

Cuts at:   2920    3628    2920 
   Size:        708    3941
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2026    2026 
   Size:       4649
 
BstBI TT'CG_AA

Cuts at:   3010    3010 
   Size:       4649
 
BstXI CCAn_nnnn'nTGG

Cuts at:    952     952 
   Size:       4649
 
BstYI r'GATC_y

Cuts at:      1    1487    1674    2141    2164    2499    2745    3051    3063 
   Size:       1486     187     467      23     335     246     306      12

Cuts at:   3063    3149    4201       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1486    1052     467     449     335     306     246     187
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    310     310 
   Size:       4649
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2599    2780    2943    2599 
   Size:        181     163    4305

  Fragments arranged by size: 

                4305     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1892    1892 
   Size:       4649
 
DraI TTT'AAA

Cuts at:    233     503    1648     233 
   Size:        270    1145    3234

  Fragments arranged by size: 

                3234    1145     270
 
DrdI GACnn_nn'nnGTC

Cuts at:   2355    3699    2355 
   Size:       1344    3305
 
EaeI y'GGCC_r

Cuts at:   2234    2408    2799    2826    4171    2234 
   Size:        174     391      27    1345    2712

  Fragments arranged by size: 

                2712    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2234    2234 
   Size:       4649
 
EarI CTCTTCn'nnn_

Cuts at:    260     967    2672    2882     260 
   Size:        707    1705     210    2027

  Fragments arranged by size: 

                2027    1705     707     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2857    3599    3745    4459    2857 
   Size:        742     146     714    3047

  Fragments arranged by size: 

                3047     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1661    1661 
   Size:       4649
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1462    2474    2906    3259    3921    1462 
   Size:       1012     432     353     662    2190

  Fragments arranged by size: 

                2190    1012     662     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:   1362    1788    1362 
   Size:        426    4223
 
EcoRI G'AATT_C

Cuts at:   1653    1653 
   Size:       4649
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2668    2668 
   Size:       4649
 
FspI TGC'GCA

Cuts at:   1610    2430    1610 
   Size:        820    3829
 
HaeII r_GCGC'y

Cuts at:    123    2083    2331    3561    4332     123 
   Size:       1960     248    1230     771     440

  Fragments arranged by size: 

                1960    1230     771     440     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    498     530    2491    2521    2523    2553     498 
   Size:         32    1961      30       2      30    2594

  Fragments arranged by size: 

                2594    1961      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1688    1688 
   Size:       4649
 
KasI G'GCGC_C

Cuts at:   2079    2327    2079 
   Size:        248    4401
 
KpnI G_GTAC'C

Cuts at:   1669    1669 
   Size:       4649
 
MluI A'CGCG_T

Cuts at:   4082    4082 
   Size:       4649
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    196     387    3408    3592     196 
   Size:        191    3021     184    1253

  Fragments arranged by size: 

                3021    1253     191     184
 
MscI TGG'CCA

Cuts at:   2410    2410 
   Size:       4649
 
MslI CAynn'nnrTG

Cuts at:   1183    1565    1601    2638    2765    1183 
   Size:        382      36    1037     127    3067

  Fragments arranged by size: 

                3067    1037     382     127      36
 
MspA1I CmG'CkG

Cuts at:   1760    2074    2434    3218    3463    1760 
   Size:        314     360     784     245    2946

  Fragments arranged by size: 

                2946     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   2830    2830 
   Size:       4649
 
NarI GG'CG_CC

Cuts at:   2080    2328    2080 
   Size:        248    4401
 
NdeI CA'TA_TG

Cuts at:   1207    1207 
   Size:       4649
 
NgoMIV G'CCGG_C

Cuts at:   2828    2828 
   Size:       4649
 
NspI r_CATG'y

Cuts at:   1606    1702    2202    3805    1606 
   Size:         96     500    1603    2450

  Fragments arranged by size: 

                2450    1603     500      96
 
PciI A'CATG_T

Cuts at:   3801    3801 
   Size:       4649
 
PfoI T'CCnGG_A

Cuts at:    112     112 
   Size:       4649
 
PmeI GTTT'AAAC

Cuts at:   1648    1648 
   Size:       4649
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3095    3127    3095 
   Size:         32    4617
 
PstI C_TGCA'G

Cuts at:    888    1696    2381     888 
   Size:        808     685    3156

  Fragments arranged by size: 

                3156     808     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4649
 
PvuII CAG'CTG

Cuts at:   2074    2434    2074 
   Size:        360    4289
 
RsrII CG'GwC_CG

Cuts at:   2844    2844 
   Size:       4649
 
SacI G_AGCT'C

Cuts at:   1663    1663 
   Size:       4649
 
SalI G'TCGA_C

Cuts at:   1686    1686 
   Size:       4649
 
SapI GCTCTTCn'nnn_

Cuts at:    967    2672    2882     967 
   Size:       1705     210    2734

  Fragments arranged by size: 

                2734    1705     210
 
SbfI CC_TGCA'GG

Cuts at:   1696    1696 
   Size:       4649
 
ScaI AGT'ACT

Cuts at:   4166    4166 
   Size:       4649
 
SfcI C'TryA_G

Cuts at:     33     873     884    1256    1692    2377    3345    3536      33 
   Size:        840      11     372     436     685     968     191    1146

  Fragments arranged by size: 

                1146     968     840     685     436     372     191      11
            
 
SfoI GGC'GCC

Cuts at:   2081    2329    2081 
   Size:        248    4401
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4649
 
SmaI CCC'GGG

Cuts at:   1453    1671    1453 
   Size:        218    4431
 
SmlI C'TyrA_G

Cuts at:   1357    1804    2719    3433    3695    1357 
   Size:        447     915     714     262    2311

  Fragments arranged by size: 

                2311     915     714     447     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4649
 
SphI G_CATG'C

Cuts at:   1702    2202    1702 
   Size:        500    4149
 
SspI AAT'ATT

Cuts at:    604     604 
   Size:       4649
 
StyI C'CwwG_G

Cuts at:    827    1623    1783    2137     827 
   Size:        796     160     354    3339

  Fragments arranged by size: 

                3339     796     354     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    433    1535    2232    2365    2824    3033     433 
   Size:       1102     697     133     459     209    2049

  Fragments arranged by size: 

                2049    1102     697     459     209     133
 
TatI w'GTAC_w

Cuts at:    687     965    4164     687 
   Size:        278    3199    1172

  Fragments arranged by size: 

                3199    1172     278
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    975    1007    4166    4198     975 
   Size:         32    3159      32    1426

  Fragments arranged by size: 

                3159    1426      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    145    2446     145 
   Size:       2301    2348
 
XbaI T'CTAG_A

Cuts at:   1680    1680 
   Size:       4649
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1115    1302    2144    1115 
   Size:        187     842    3620

  Fragments arranged by size: 

                3620     842     187
 
XmaI C'CCGG_G

Cuts at:   1451    1669    1451 
   Size:        218    4431
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NspI
     PciI     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI    BseRI     BsgI    BsiWI    BsmBI   BssHII   BstEII
  BstZ17I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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