Restriction map for pF1KB4745
 (Circular) (Six-base) MAPSORT of: pF1KB4745.seq  Check: 7444  from: 1  to: 4232



pF1KB4745  4232 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:    947    1270     947 
   Size:        323    3909
 
Acc65I G'GTAC_C

Cuts at:   1248    1248 
   Size:       4232
 
AclI AA'CG_TT

Cuts at:   4072    4072 
   Size:       4232
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    388    2057    2489    2842    3504     388 
   Size:       1669     432     353     662    1116

  Fragments arranged by size: 

                1669    1116     662     432     353
 
AfeI AGC'GCT

Cuts at:    529     529 
   Size:       4232
 
AflIII A'CryG_T

Cuts at:   1584    3384    3665    1584 
   Size:       1800     281    2151

  Fragments arranged by size: 

                2151    1800     281
 
AleI CACnn'nnGTG

Cuts at:    805     805 
   Size:       4232
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    271     303     271 
   Size:         32    4200
 
AlwNI CAG_nnn'CTG

Cuts at:    204     717    1537    2975     204 
   Size:        513     820    1438    1461

  Fragments arranged by size: 

                1461    1438     820     513
 
ApaI G_GGCC'C

Cuts at:    449     449 
   Size:       4232
 
ApaLI G'TGCA_C

Cuts at:   1079    3070    1079 
   Size:       1991    2241
 
ApoI r'AATT_y

Cuts at:   1236    3585    3841    1236 
   Size:       2349     256    1627

  Fragments arranged by size: 

                2349    1627     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4232
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4232
 
AvaI C'yCGr_G

Cuts at:    453    1252     453 
   Size:        799    3433
 
BamHI G'GATC_C

Cuts at:   1257    1257 
   Size:       4232
 
BanI G'GyrC_C

Cuts at:   1248    1662    1910    1945    1248 
   Size:        414     248      35    3535

  Fragments arranged by size: 

                3535     414     248      35
 
BanII G_rGCy'C

Cuts at:    141     449     659    1246    2276     141 
   Size:        308     210     587    1030    2097

  Fragments arranged by size: 

                2097    1030     587     308     210
 
BbeI G_GCGC'C

Cuts at:   1666    1914    1666 
   Size:        248    3984
 
BciVI GTATCCnnnnn_n'

Cuts at:   2130    3186    4232    2130 
   Size:       1056    1046    2130

  Fragments arranged by size: 

                2130    1056    1046
 
BclI T'GATC_A

Cuts at:   1752    1752 
   Size:       4232
 
BglII A'GATC_T

Cuts at:   1747    3784    1747 
   Size:       2037    2195
 
BlpI GC'TnA_GC

Cuts at:   1344    1344 
   Size:       4232
 
Bme1580I G_kGCm'C

Cuts at:    449    1083    1857    1950    3074     449 
   Size:        634     774      93    1124    1607

  Fragments arranged by size: 

                1607    1124     774     634      93
 
BmgBI CAC'GTC

Cuts at:    972     972 
   Size:       4232
 
BmrI ACTGGGnnnn_n'

Cuts at:    452    1444    1525    1860    4004     452 
   Size:        992      81     335    2144     680

  Fragments arranged by size: 

                2144     992     680     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1235    2599    1235 
   Size:       1364    2868
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     763     907    1045     587 
   Size:        176     144     138    3774

  Fragments arranged by size: 

                3774     176     144     138
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1408    2287    3001    3299    1408 
   Size:        879     714     298    2341

  Fragments arranged by size: 

                2341     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    126    3936     126 
   Size:       3810     422
 
BsaAI yAC'GTr

Cuts at:    147    1585     147 
   Size:       1438    2794
 
BsaBI GATnn'nnATC

Cuts at:      6    1746    3783       6 
   Size:       1740    2037     455

  Fragments arranged by size: 

                2037    1740     455
 
BsaHI Gr'CG_yC

Cuts at:    979    1663    1911    2613    4109     979 
   Size:        684     248     702    1496    1102

  Fragments arranged by size: 

                1496    1102     702     684     248
 
BsaWI w'CCGG_w

Cuts at:    698    1421    1942    2620    3031    3178    3452     698 
   Size:        723     521     678     411     147     274    1478

  Fragments arranged by size: 

                1478     723     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    271     301     271 
   Size:         30    4202
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    569     685     760     569 
   Size:        116      75    4041

  Fragments arranged by size: 

                4041     116      75
 
BseYI C'CCAG_C

Cuts at:   1657    2415    3080    3488    1657 
   Size:        758     665     408    2401

  Fragments arranged by size: 

                2401     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    127     730     805     816     127 
   Size:        603      75      11    3543

  Fragments arranged by size: 

                3543     603      75      11
 
BsiEI CG_ry'CG

Cuts at:    104    1774    1820    3050     104 
   Size:       1670      46    1230    1286

  Fragments arranged by size: 

                1670    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    141     659     903     920    1083    1246    2024    2214    3074 
   Size:        518     244      17     163     163     778     190     860

Cuts at:   3074     141 
   Size:       1299

  Fragments arranged by size: 

                1299     860     778     518     244     190     163     163
                  17
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1194    1194 
   Size:       4232
 
Bsp1286I G_dGCh'C

Cuts at:    141     449     659     903     920    1083    1246    1857    1950 
   Size:        308     210     244      17     163     163     611      93

Cuts at:   1950    2024    2214    2276    3074     141 
   Size:         74     190      62     798    1299

  Fragments arranged by size: 

                1299     798     611     308     244     210     190     163
                 163      93      74      62      17
 
BspEI T'CCGG_A

Cuts at:    698     698 
   Size:       4232
 
BspHI T'CATG_A

Cuts at:   2664    2664 
   Size:       4232
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    510    1282    1496    1798    2179     510 
   Size:        772     214     302     381    2563

  Fragments arranged by size: 

                2563     772     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     729    2524    2578    4062    4227      50 
   Size:        679    1795      54    1484     165      55

  Fragments arranged by size: 

                1795    1484     679     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2144    2144 
   Size:       4232
 
BsrFI r'CCGG_y

Cuts at:    835    1000    1148    2230    2411     835 
   Size:        165     148    1082     181    2656

  Fragments arranged by size: 

                2656    1082     181     165     148
 
BssSI C'ACGA_G

Cuts at:   2503    3211    2503 
   Size:        708    3524
 
BstAPI GCAn_nnn'nTGC

Cuts at:    717    1609     717 
   Size:        892    3340
 
BstBI TT'CG_AA

Cuts at:   2593    2593 
   Size:       4232
 
BstXI CCAn_nnnn'nTGG

Cuts at:    819    1132     819 
   Size:        313    3919
 
BstYI r'GATC_y

Cuts at:      1     689    1257    1724    1747    2082    2328    2634    2646 
   Size:        688     568     467      23     335     246     306      12

Cuts at:   2646    2732    3784       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     688     568     467     449     335     306     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     275     431    1022    1100     107 
   Size:        168     156     591      78    3239

  Fragments arranged by size: 

                3239     591     168     156      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1002    2182    2363    2526    1002 
   Size:       1180     181     163    2708

  Fragments arranged by size: 

                2708    1180     181     163
 
BtsI GCAGTG_nn'

Cuts at:    900    1475     900 
   Size:        575    3657
 
DraI TTT'AAA

Cuts at:   1231    1231 
   Size:       4232
 
DraIII CAC_nnn'GTG

Cuts at:    150     255     150 
   Size:        105    4127
 
DrdI GACnn_nn'nnGTC

Cuts at:   1186    1938    3282    1186 
   Size:        752    1344    2136

  Fragments arranged by size: 

                2136    1344     752
 
EaeI y'GGCC_r

Cuts at:    428     809    1025    1817    1991    2382    2409    3754     428 
   Size:        381     216     792     174     391      27    1345     906

  Fragments arranged by size: 

                1345     906     792     391     381     216     174      27
            
 
EagI C'GGCC_G

Cuts at:   1817    1817 
   Size:       4232
 
EarI CTCTTCn'nnn_

Cuts at:   2255    2465    2255 
   Size:        210    4022
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    846    2440    3182    3328    4042     846 
   Size:       1594     742     146     714    1036

  Fragments arranged by size: 

                1594    1036     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    139     657    1244     139 
   Size:        518     587    3127

  Fragments arranged by size: 

                3127     587     518
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    388     587     763     907    1045    2057    2489    2842    3504 
   Size:        199     176     144     138    1012     432     353     662

Cuts at:   3504     388 
   Size:       1116

  Fragments arranged by size: 

                1116    1012     662     432     353     199     176     144
                 138
 
EcoNI CCTnn'n_nnAGG

Cuts at:    700     700 
   Size:       4232
 
EcoO109I rG'GnC_Cy

Cuts at:    113     201     402     446    1179    1371     113 
   Size:         88     201      44     733     192    2974

  Fragments arranged by size: 

                2974     733     201     192      88      44
 
EcoRI G'AATT_C

Cuts at:   1236    1236 
   Size:       4232
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    258     351    2251     258 
   Size:         93    1900    2239

  Fragments arranged by size: 

                2239    1900      93
 
FspI TGC'GCA

Cuts at:   2013    2013 
   Size:       4232
 
HaeII r_GCGC'y

Cuts at:    531    1666    1914    3144    3915     531 
   Size:       1135     248    1230     771     848

  Fragments arranged by size: 

                1230    1135     848     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    761     793     978    1010    2074    2104    2106    2136     761 
   Size:         32     185      32    1064      30       2      30    2857

  Fragments arranged by size: 

                2857    1064     185      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1038    1271    1038 
   Size:        233    3999
 
KasI G'GCGC_C

Cuts at:   1662    1910    1662 
   Size:        248    3984
 
KpnI G_GTAC'C

Cuts at:   1252    1252 
   Size:       4232
 
MluI A'CGCG_T

Cuts at:   3665    3665 
   Size:       4232
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2991    3175    2991 
   Size:        184    4048
 
MscI TGG'CCA

Cuts at:    430     811    1027    1993     430 
   Size:        381     216     966    2669

  Fragments arranged by size: 

                2669     966     381     216
 
MslI CAynn'nnrTG

Cuts at:    223     251     544     781     805    1021    2221    2348     223 
   Size:         28     293     237      24     216    1200     127    2107

  Fragments arranged by size: 

                2107    1200     293     237     216     127      28      24
            
 
MspA1I CmG'CkG

Cuts at:    195     714    1053    1343    1657    2017    2801    3046     195 
   Size:        519     339     290     314     360     784     245    1381

  Fragments arranged by size: 

                1381     784     519     360     339     314     290     245
            
 
NaeI GCC'GGC

Cuts at:   1002    1150    2413    1002 
   Size:        148    1263    2821

  Fragments arranged by size: 

                2821    1263     148
 
NarI GG'CG_CC

Cuts at:   1663    1911    1663 
   Size:        248    3984
 
NcoI C'CATG_G

Cuts at:    107    1100     107 
   Size:        993    3239
 
NgoMIV G'CCGG_C

Cuts at:   1000    1148    2411    1000 
   Size:        148    1263    2821

  Fragments arranged by size: 

                2821    1263     148
 
NspI r_CATG'y

Cuts at:   1285    1785    3388    1285 
   Size:        500    1603    2129

  Fragments arranged by size: 

                2129    1603     500
 
PciI A'CATG_T

Cuts at:   3384    3384 
   Size:       4232
 
PflMI CCAn_nnn'nTGG

Cuts at:    255     255 
   Size:       4232
 
PmeI GTTT'AAAC

Cuts at:   1231    1231 
   Size:       4232
 
PmlI CAC'GTG

Cuts at:    147     147 
   Size:       4232
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    271     303    2678    2710     271 
   Size:         32    2375      32    1793

  Fragments arranged by size: 

                2375    1793      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    113    1179     113 
   Size:       1066    3166
 
PspOMI G'GGCC_C

Cuts at:    445     445 
   Size:       4232
 
PstI C_TGCA'G

Cuts at:    200     392    1279    1964     200 
   Size:        192     887     685    2468

  Fragments arranged by size: 

                2468     887     685     192
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4232
 
PvuII CAG'CTG

Cuts at:    195     714    1657    2017     195 
   Size:        519     943     360    2410

  Fragments arranged by size: 

                2410     943     519     360
 
RsrII CG'GwC_CG

Cuts at:   2427    2427 
   Size:       4232
 
SacI G_AGCT'C

Cuts at:    141     659    1246     141 
   Size:        518     587    3127

  Fragments arranged by size: 

                3127     587     518
 
SalI G'TCGA_C

Cuts at:   1269    1269 
   Size:       4232
 
SanDI GG'GwC_CC

Cuts at:    113     113 
   Size:       4232
 
SapI GCTCTTCn'nnn_

Cuts at:   2255    2465    2255 
   Size:        210    4022
 
SbfI CC_TGCA'GG

Cuts at:   1279    1279 
   Size:       4232
 
ScaI AGT'ACT

Cuts at:   3749    3749 
   Size:       4232
 
SexAI A'CCwGG_T

Cuts at:    818     818 
   Size:       4232
 
SfcI C'TryA_G

Cuts at:     33     196     388    1275    1960    2928    3119      33 
   Size:        163     192     887     685     968     191    1146

  Fragments arranged by size: 

                1146     968     887     685     192     191     163
 
SfoI GGC'GCC

Cuts at:   1664    1912    1664 
   Size:        248    3984
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4232
 
SmaI CCC'GGG

Cuts at:    455    1254     455 
   Size:        799    3433
 
SmlI C'TyrA_G

Cuts at:   1387    2302    3016    3278    1387 
   Size:        915     714     262    2341

  Fragments arranged by size: 

                2341     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4232
 
SphI G_CATG'C

Cuts at:   1285    1785    1285 
   Size:        500    3732
 
StuI AGG'CCT

Cuts at:    499    1066     499 
   Size:        567    3665
 
StyI C'CwwG_G

Cuts at:    107    1100    1366    1720     107 
   Size:        993     266     354    2619

  Fragments arranged by size: 

                2619     993     354     266
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    148    1815    1948    2407    2616     148 
   Size:       1667     133     459     209    1764

  Fragments arranged by size: 

                1764    1667     459     209     133
 
TatI w'GTAC_w

Cuts at:    773    3747     773 
   Size:       2974    1258
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1073    1105    3749    3781    1073 
   Size:         32    2644      32    1524

  Fragments arranged by size: 

                2644    1524      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2029    2029 
   Size:       4232
 
XbaI T'CTAG_A

Cuts at:   1263    1263 
   Size:       4232
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1020    1727    1020 
   Size:        707    3525
 
XmaI C'CCGG_G

Cuts at:    453    1252     453 
   Size:        799    3433
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AhdI     AlfI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI     BmtI   Bpu10I
    BsiWI     BsmI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI    PshAI
     PsiI    PspXI     PsrI    SacII     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     XmnI     ZraI
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