Restriction map for pF1KB4737
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
BamHI       BbeI        BcgI        BfrI        BglII       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssHII      BstBI       ClaI        
CpoI        DraI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FseI        FspI        HaeII       HindIII     HpaI        
KasI        KpnI        Ksp632I     MamI        MluI        
MroI        NarI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        ScaI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SwaI        Tth111I     XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI         4063  GAATTC               [GA]^AATT[CT]
AcyI         1243  GGCGTC               G[GA]^CG[CT]C
Alw44I       2882  GTGCAC               G^TGCAC
ApaLI        2882  GTGCAC               G^TGCAC
ApoI         4063  GAATTC               [GA]^AATT[CT]
Asp700I      2446  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        3154  CCTAGG               C^CTAGG
BalI         3779  TGGCCA               TGG^CCA
BbrPI         119  CACGTG               CAC^GTG
BbvCI        3904  GCTGAGG              CCTCAGC(-5/-2)
BclI         3117  TGATCA               T^GATCA
BlnI         3154  CCTAGG               C^CTAGG
BsaAI         119  CACGTG               [CT]AC^GT[GA]
BsaHI        1243  GGCGTC               G[GA]^CG[CT]C
BsrBI        1068  GAGCGG               CCGCTC(-3/-3)
Bst1107I     3577  GTATAC               GTA^TAC
BstAPI       3084  GCAAGGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        3740  CCAAGGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1162  GGATCT               [GA]^GATC[CT]
BstZ17I      3577  GTATAC               GTA^TAC
EcoRI        4063  GAATTC               G^AATTC
FbaI         3117  TGATCA               T^GATCA
HgaI         1244  GCGTC                GACGC(5/10)
Hin1I        1243  GGCGTC               G[GA]^CG[CT]C
KspI          978  CCGCGG               CCGC^GG
MfeI         1436  CAATTG               C^AATTG
MflI         1162  GGATCT               [GA]^GATC[CT]
MluNI        3779  TGGCCA               TGG^CCA
MscI         3779  TGGCCA               TGG^CCA
MslI         3109  CACCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1436  CAATTG               C^AATTG
NruI         2811  TCGCGA               TCG^CGA
PaeR7I       2631  CTCGAG               C^TCGAG
PflMI        3638  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         119  CACGTG               CAC^GTG
PmlI          119  CACGTG               CAC^GTG
PshAI        2470  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         3654  GAGCTC               GAGCT^C
SacII         978  CCGCGG               CCGC^GG
SbfI          382  CCTGCAGG             CCTGCA^GG
Sse8387I      382  CCTGCAGG             CCTGCA^GG
SspI         2857  AATATT               AAT^ATT
TfiI         1478  GATTC                G^A[AT]TC
Van91I       3638  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3463  TCTAGA               T^CTAGA
XhoI         2631  CTCGAG               C^TCGAG
XhoII        1162  GGATCT               [GA]^GATC[CT]
XmnI         2446  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         2648  GTCTAC               GT^[AC][GT]AC
             3577  GTATAC              
AfaI         2558  GTAC                 GT^AC
             3215  GTAC                
AflIII        669  ACGTGT               A^C[GA][CT]GT
             3672  ACACGT              
AlwI         1507  GATCC                GGATC(4/5)
             1162  GGATC               
BciVI          15  GGATAC               GTATCC(6/5)
             3608  GTATCC              
BlpI            3  GCTCAGC              GC^T[ACGT]AGC
             3368  GCTCAGC             
Bpu1102I        3  GCTCAGC              GC^T[ACGT]AGC
             3368  GCTCAGC             
BsgI          308  GTGCAG               GTGCAG(16/14)
             2504  GTGCAG              
BsmI         3008  GCATTC               GAATGC(1/-1)
             3233  GCATTC              
BsrDI        2694  CATTGC               GCAATG(2/0)
             3825  CATTGC              
CelII           3  GCTCAGC              GC^T[ACGT]AGC
             3368  GCTCAGC             
CfoI         2841  GCGC                 GCG^C
             3771  GCGC                
EaeI         2484  TGGCCG               [CT]^GGCC[GA]
             3779  TGGCCA              
EcoNI        3544  CCTAACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3855  CCTGAAAAAGG         
HhaI         2841  GCGC                 GCG^C
             3771  GCGC                
HinP1I       2841  GCGC                 G^CGC
             3771  GCGC                
MseI          283  TTAA                 T^TAA
             3064  TTAA                
NaeI         1168  GCCGGC               GCC^GGC
             2698  GCCGGC              
NcoI         1725  CCATGG               C^CATGG
             3111  CCATGG              
NgoMIV       1168  GCCGGC               G^CCGGC
             2698  GCCGGC              
NspI           61  ACATGC               [GA]CATG^[CT]
             3484  ACATGC              
PstI          383  CTGCAG               CTGCA^G
             2876  CTGCAG              
RsaI         2558  GTAC                 GT^AC
             3215  GTAC                
StuI          864  AGGCCT               AGG^CCT
             1426  AGGCCT              
Tru9I         283  TTAA                 T^TAA
             3064  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AocI        
ApaI        AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BssSI       BstEII      
BstF5I      BstNI       BstPI       BstUI       Bsu36I      
Cac8I       Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        Eco81I      
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PvuII       Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SmlI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com