Restriction map for
pF1KB4737
(Linear) (Six-base) MAPSORT of: KIBB4737.seq Check: 6043 from: 1 to: 4098
KIBB4737.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 376 2869 4098
Size: 376 2493 1229
Fragments arranged by size:
2493 1229 376
AccI GT'mk_AC
Cuts at: 0 2649 3578 4098
Size: 2649 929 520
Fragments arranged by size:
2649 929 520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3436 3476 4098
Size: 3436 40 622
Fragments arranged by size:
3436 622 40
AflIII A'CryG_T
Cuts at: 0 669 3672 4098
Size: 669 3003 426
Fragments arranged by size:
3003 669 426
AleI CACnn'nnGTG
Cuts at: 0 3113 4098
Size: 3113 985
AlwNI CAG_nnn'CTG
Cuts at: 0 1570 1668 1849 4098
Size: 1570 98 181 2249
Fragments arranged by size:
2249 1570 181 98
ApaI G_GGCC'C
Cuts at: 0 100 1103 1255 2715 3047 3163 3618 4098
Size: 100 1003 152 1460 332 116 455 480
Fragments arranged by size:
1460 1003 480 455 332 152 116 100
ApaLI G'TGCA_C
Cuts at: 0 2882 4098
Size: 2882 1216
ApoI r'AATT_y
Cuts at: 0 4063 4098
Size: 4063 35
AvaI C'yCGr_G
Cuts at: 0 1553 2120 2631 4098
Size: 1553 567 511 1467
Fragments arranged by size:
1553 1467 567 511
AvrII C'CTAG_G
Cuts at: 0 3154 4098
Size: 3154 944
BanI G'GyrC_C
Cuts at: 0 604 676 694 775 919 1018 2044 4098
Size: 604 72 18 81 144 99 1026 2054
Fragments arranged by size:
2054 1026 604 144 99 81 72 18
BanII G_rGCy'C
Cuts at: 0 100 321 645 960 1088 1103 1255 2715
Size: 100 221 324 315 128 15 152 1460
Cuts at: 2715 3032 3047 3163 3618 3658 3883 4098
Size: 317 15 116 455 40 225 215
Fragments arranged by size:
1460 455 324 317 315 225 221 215
152 128 116 100 40 15 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 850 1210 1345 2587 3046 4098
Size: 850 360 135 1242 459 1052
Fragments arranged by size:
1242 1052 850 459 360 135
BbvCI CC'TCA_GC
Cuts at: 0 3905 4098
Size: 3905 193
BciVI GTATCCnnnnn_n'
Cuts at: 0 9 3619 4098
Size: 9 3610 479
Fragments arranged by size:
3610 479 9
BclI T'GATC_A
Cuts at: 0 3117 4098
Size: 3117 981
BglI GCCn_nnn'nGGC
Cuts at: 0 149 413 764 872 944 1412 1439 3005
Size: 149 264 351 108 72 468 27 1566
Cuts at: 3005 3266 4098
Size: 261 832
Fragments arranged by size:
1566 832 468 351 264 261 149 108
72 27
BlpI GC'TnA_GC
Cuts at: 0 4 3369 4098
Size: 4 3365 729
Fragments arranged by size:
3365 729 4
Bme1580I G_kGCm'C
Cuts at: 0 100 609 681 699 780 1103 1255 1894
Size: 100 509 72 18 81 323 152 639
Cuts at: 1894 2049 2715 2886 3047 3163 3618 4098
Size: 155 666 171 161 116 455 480
Fragments arranged by size:
666 639 509 480 455 323 171 161
155 152 116 100 81 72 18
BmgBI CAC'GTC
Cuts at: 0 670 3675 4098
Size: 670 3005 423
Fragments arranged by size:
3005 670 423
BmrI ACTGGGnnnn_n'
Cuts at: 0 1115 4078 4098
Size: 1115 2963 20
Fragments arranged by size:
2963 1115 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1107 4098
Size: 1107 2991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 611 746 920 1160 1244 1307 1469
Size: 335 276 135 174 240 84 63 162
Cuts at: 1469 1547 1637 1973 2081 2513 2564 2567 2765
Size: 78 90 336 108 432 51 3 198
Cuts at: 2765 3284 4098
Size: 519 814
Fragments arranged by size:
814 519 432 336 335 276 240 198
174 162 135 108 90 84 78 63
51 3
Bpu10I CC'TnA_GC
Cuts at: 0 1589 3905 4098
Size: 1589 2316 193
Fragments arranged by size:
2316 1589 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 3516 4098
Size: 314 3202 582
Fragments arranged by size:
3202 582 314
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 1339 1726 1744 1987 2626 3433 4098
Size: 220 1119 387 18 243 639 807 665
Fragments arranged by size:
1119 807 665 639 387 243 220 18
BsaAI yAC'GTr
Cuts at: 0 121 4098
Size: 121 3977
BsaHI Gr'CG_yC
Cuts at: 0 1244 4098
Size: 1244 2854
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 165 195 3510 3540 4098
Size: 165 30 3315 30 558
Fragments arranged by size:
3315 558 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 482 1472 2237 3856 4098
Size: 482 990 765 1619 242
Fragments arranged by size:
1619 990 765 482 242
BseYI C'CCAG_C
Cuts at: 0 99 687 952 1096 2086 2289 2437 3474
Size: 99 588 265 144 990 203 148 1037
Cuts at: 3474 3633 4098
Size: 159 465
Fragments arranged by size:
1037 990 588 465 265 203 159 148
144 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 2525 4098
Size: 329 2196 1573
Fragments arranged by size:
2196 1573 329
BsiHKAI G_wGCw'C
Cuts at: 0 393 492 582 1554 1563 1590 2022 2031
Size: 393 99 90 972 9 27 432 9
Cuts at: 2031 2607 2760 2886 3129 3658 4098
Size: 576 153 126 243 529 440
Fragments arranged by size:
972 576 529 440 432 393 243 153
126 99 90 27 9 9
BsmI GAATG_Cn'
Cuts at: 0 3008 3233 4098
Size: 3008 225 865
Fragments arranged by size:
3008 865 225
Bsp1286I G_dGCh'C
Cuts at: 0 100 321 393 492 582 609 645 681
Size: 100 221 72 99 90 27 36 36
Cuts at: 681 699 780 960 1088 1103 1255 1554 1563
Size: 18 81 180 128 15 152 299 9
Cuts at: 1563 1590 1894 2022 2031 2049 2607 2715 2760
Size: 27 304 128 9 18 558 108 45
Cuts at: 2760 2886 3032 3047 3129 3163 3618 3658 3883
Size: 126 146 15 82 34 455 40 225
Cuts at: 3883 4098
Size: 215
Fragments arranged by size:
558 455 304 299 225 221 215 180
152 146 128 128 126 108 100 99
90 82 81 72 45 40 36 36
34 27 27 18 18 15 15 9
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 354 376 2869 3234 3908 4098
Size: 354 22 2493 365 674 190
Fragments arranged by size:
2493 674 365 354 190 22
BsrBI CCG'CTC
Cuts at: 0 1070 4098
Size: 1070 3028
BsrDI GCAATG_nn'
Cuts at: 0 2693 3824 4098
Size: 2693 1131 274
Fragments arranged by size:
2693 1131 274
BsrFI r'CCGG_y
Cuts at: 0 835 1006 1168 1798 2698 4098
Size: 835 171 162 630 900 1400
Fragments arranged by size:
1400 900 835 630 171 162
BssSI C'ACGA_G
Cuts at: 0 392 491 1211 2021 2462 4098
Size: 392 99 720 810 441 1636
Fragments arranged by size:
1636 810 720 441 392 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3090 4098
Size: 3090 1008
BstEII G'GTnAC_C
Cuts at: 0 433 1999 2071 2467 2845 3106 3250 4098
Size: 433 1566 72 396 378 261 144 848
Fragments arranged by size:
1566 848 433 396 378 261 144 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3747 4098
Size: 3747 351
BstYI r'GATC_y
Cuts at: 0 1162 4098
Size: 1162 2936
BstZ17I GTA'TAC
Cuts at: 0 3579 4098
Size: 3579 519
Bsu36I CC'TnA_GG
Cuts at: 0 1680 2237 2588 3245 4098
Size: 1680 557 351 657 853
Fragments arranged by size:
1680 853 657 557 351
BtgI C'CryG_G
Cuts at: 0 645 699 725 843 978 1725 2715 2903
Size: 645 54 26 118 135 747 990 188
Cuts at: 2903 2931 3111 3137 4098
Size: 28 180 26 961
Fragments arranged by size:
990 961 747 645 188 180 135 118
54 28 26 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2828 4098
Size: 2828 1270
BtsI GCAGTG_nn'
Cuts at: 0 711 1892 3528 3810 4098
Size: 711 1181 1636 282 288
Fragments arranged by size:
1636 1181 711 288 282
DraIII CAC_nnn'GTG
Cuts at: 0 307 487 1612 4098
Size: 307 180 1125 2486
Fragments arranged by size:
2486 1125 307 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 548 1349 2384 4098
Size: 548 801 1035 1714
Fragments arranged by size:
1714 1035 801 548
EaeI y'GGCC_r
Cuts at: 0 2484 3779 4098
Size: 2484 1295 319
Fragments arranged by size:
2484 1295 319
EcoICRI GAG'CTC
Cuts at: 0 3656 4098
Size: 3656 442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 611 746 920 1160 1244 1307 1469
Size: 335 276 135 174 240 84 63 162
Cuts at: 1469 1547 1637 1973 2081 2513 2564 2567 2765
Size: 78 90 336 108 432 51 3 198
Cuts at: 2765 3284 3436 3476 4098
Size: 519 152 40 622
Fragments arranged by size:
622 519 432 336 335 276 240 198
174 162 152 135 108 90 84 78
63 51 40 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3548 3859 4098
Size: 3548 311 239
Fragments arranged by size:
3548 311 239
EcoO109I rG'GnC_Cy
Cuts at: 0 96 142 178 289 325 343 721 730
Size: 96 46 36 111 36 18 378 9
Cuts at: 730 1100 1108 1144 1180 1252 1297 1342 1396
Size: 370 8 36 36 72 45 45 54
Cuts at: 1396 1495 1567 1594 1639 1684 1828 1846 2233
Size: 99 72 27 45 45 144 18 387
Cuts at: 2233 2269 2341 2395 2539 2584 2711 2908 2989
Size: 36 72 54 144 45 127 197 81
Cuts at: 2989 3043 3142 3159 3160 3241 3259 4098
Size: 54 99 17 1 81 18 839
Fragments arranged by size:
839 387 378 370 197 144 144 127
111 99 99 96 81 81 72 72
72 54 54 54 46 45 45 45
45 45 36 36 36 36 36 27
18 18 18 17 9 8 1
EcoRI G'AATT_C
Cuts at: 0 4063 4098
Size: 4063 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 874 2467 4098
Size: 874 1593 1631
Fragments arranged by size:
1631 1593 874
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1185 1217 1229 1261 3677 3709 3934 3966
Size: 1185 32 12 32 2416 32 225 32
Cuts at: 3966 4098
Size: 132
Fragments arranged by size:
2416 1185 225 132 32 32 32 32
12
HincII GTy'rAC
Cuts at: 0 2200 2751 2821 4098
Size: 2200 551 70 1277
Fragments arranged by size:
2200 1277 551 70
MfeI C'AATT_G
Cuts at: 0 1436 4098
Size: 1436 2662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 456 861 2256 3201 4098
Size: 237 219 405 1395 945 897
Fragments arranged by size:
1395 945 897 405 237 219
MscI TGG'CCA
Cuts at: 0 3781 4098
Size: 3781 317
MslI CAynn'nnrTG
Cuts at: 0 3113 4098
Size: 3113 985
MspA1I CmG'CkG
Cuts at: 0 313 403 691 980 1051 1384 1447 1645
Size: 313 90 288 289 71 333 63 198
Cuts at: 1645 1690 2293 4098
Size: 45 603 1805
Fragments arranged by size:
1805 603 333 313 289 288 198 90
71 63 45
NaeI GCC'GGC
Cuts at: 0 1170 2700 4098
Size: 1170 1530 1398
Fragments arranged by size:
1530 1398 1170
NcoI C'CATG_G
Cuts at: 0 1725 3111 4098
Size: 1725 1386 987
Fragments arranged by size:
1725 1386 987
NgoMIV G'CCGG_C
Cuts at: 0 1168 2698 4098
Size: 1168 1530 1400
Fragments arranged by size:
1530 1400 1168
NruI TCG'CGA
Cuts at: 0 2813 4098
Size: 2813 1285
NspI r_CATG'y
Cuts at: 0 65 3488 4098
Size: 65 3423 610
Fragments arranged by size:
3423 610 65
PasI CC'CwG_GG
Cuts at: 0 1321 1843 2788 4098
Size: 1321 522 945 1310
Fragments arranged by size:
1321 1310 945 522
PflMI CCAn_nnn'nTGG
Cuts at: 0 3644 4098
Size: 3644 454
PfoI T'CCnGG_A
Cuts at: 0 510 897 1176 1221 1599 2742 3291 4098
Size: 510 387 279 45 378 1143 549 807
Fragments arranged by size:
1143 807 549 510 387 378 279 45
PmlI CAC'GTG
Cuts at: 0 121 4098
Size: 121 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1967 1999 4098
Size: 1967 32 2099
Fragments arranged by size:
2099 1967 32
PpuMI rG'GwC_Cy
Cuts at: 0 289 343 721 730 1108 1180 1297 1342
Size: 289 54 378 9 378 72 117 45
Cuts at: 1342 1396 1567 1594 1639 1684 1828 2395 2539
Size: 54 171 27 45 45 144 567 144
Cuts at: 2539 2584 2908 2989 3142 4098
Size: 45 324 81 153 956
Fragments arranged by size:
956 567 378 378 324 289 171 153
144 144 117 81 72 54 54 45
45 45 45 27 9
PshAI GACnn'nnGTC
Cuts at: 0 2474 4098
Size: 2474 1624
PspOMI G'GGCC_C
Cuts at: 0 96 1099 1251 2711 3043 3159 3614 4098
Size: 96 1003 152 1460 332 116 455 484
Fragments arranged by size:
1460 1003 484 455 332 152 116 96
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1191 1223 2633 2665 3790 3822 4098
Size: 1191 32 1410 32 1125 32 276
Fragments arranged by size:
1410 1191 1125 276 32 32 32
PstI C_TGCA'G
Cuts at: 0 387 2880 4098
Size: 387 2493 1218
Fragments arranged by size:
2493 1218 387
PvuII CAG'CTG
Cuts at: 0 313 403 691 1051 2293 4098
Size: 313 90 288 360 1242 1805
Fragments arranged by size:
1805 1242 360 313 288 90
SacI G_AGCT'C
Cuts at: 0 3658 4098
Size: 3658 440
SacII CC_GC'GG
Cuts at: 0 981 4098
Size: 981 3117
SanDI GG'GwC_CC
Cuts at: 0 721 1342 1639 2539 4098
Size: 721 621 297 900 1559
Fragments arranged by size:
1559 900 721 621 297
SbfI CC_TGCA'GG
Cuts at: 0 387 4098
Size: 387 3711
SexAI A'CCwGG_T
Cuts at: 0 192 303 366 456 600 1869 1977 2202
Size: 192 111 63 90 144 1269 108 225
Cuts at: 2202 2652 2679 2886 4098
Size: 450 27 207 1212
Fragments arranged by size:
1269 1212 450 225 207 192 144 111
108 90 63 27
SfcI C'TryA_G
Cuts at: 0 383 2039 2876 3348 3874 4098
Size: 383 1656 837 472 526 224
Fragments arranged by size:
1656 837 526 472 383 224
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 872 1439 3266 4098
Size: 872 567 1827 832
Fragments arranged by size:
1827 872 832 567
SmlI C'TyrA_G
Cuts at: 0 329 2631 3495 4098
Size: 329 2302 864 603
Fragments arranged by size:
2302 864 603 329
SspI AAT'ATT
Cuts at: 0 2859 4098
Size: 2859 1239
StuI AGG'CCT
Cuts at: 0 866 1428 4098
Size: 866 562 2670
Fragments arranged by size:
2670 866 562
StyI C'CwwG_G
Cuts at: 0 339 788 923 1104 1725 2472 3111 3154
Size: 339 449 135 181 621 747 639 43
Cuts at: 3154 3255 3389 3549 3659 3740 4098
Size: 101 134 160 110 81 358
Fragments arranged by size:
747 639 621 449 358 339 181 160
135 134 110 101 81 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2140 2662 3524 4098
Size: 2140 522 862 574
Fragments arranged by size:
2140 862 574 522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 165 197 1491 1523 1884 1916 2568 2600
Size: 165 32 1294 32 361 32 652 32
Cuts at: 2600 3192 3224 4098
Size: 592 32 874
Fragments arranged by size:
1294 874 652 592 361 165 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 3463 4098
Size: 3463 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 346 1111 1669 1732 2479 3262 3641 4098
Size: 346 765 558 63 747 783 379 457
Fragments arranged by size:
783 765 747 558 457 379 346 63
XhoI C'TCGA_G
Cuts at: 0 2631 4098
Size: 2631 1467
XmnI GAAnn'nnTTC
Cuts at: 0 2450 4098
Size: 2450 1648
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI AvrII BanI BanII BbsI BbvCI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HincII MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NruI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuII SacI SacII SanDI SbfI SexAI SfcI
SfiI SmlI SspI StuI StyI TaqII TstI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI BaeI BamHI BbeI BcgI
BfrBI BglII BmtI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BstBI ClaI CspCI DraI
EagI EarI EciI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI NarI NdeI NheI
NotI NsiI PacI PciI PmeI PsiI PspXI PvuI
RsrII SalI SapI ScaI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TatI Tth111I XmaI
ZraI