Restriction map for pF1KB4712
 (Circular) (Six-base) MAPSORT of: pF1KB4712.seq  Check: 9122  from: 1  to: 3854



pF1KB4712  3854 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:    892     892 
   Size:       3854
 
Acc65I G'GTAC_C

Cuts at:    870     870 
   Size:       3854
 
AclI AA'CG_TT

Cuts at:    260    3694     260 
   Size:       3434     420
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    499     533    1679    2111    2464    3126     499 
   Size:         34    1146     432     353     662    1227

  Fragments arranged by size: 

                1227    1146     662     432     353      34
 
AfeI AGC'GCT

Cuts at:    163     163 
   Size:       3854
 
AflIII A'CryG_T

Cuts at:   1206    3006    3287    1206 
   Size:       1800     281    1773

  Fragments arranged by size: 

                1800    1773     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1159    2597    1159 
   Size:       1438    2416
 
ApaLI G'TGCA_C

Cuts at:   2692    2692 
   Size:       3854
 
ApoI r'AATT_y

Cuts at:    858    3207    3463     858 
   Size:       2349     256    1249

  Fragments arranged by size: 

                2349    1249     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3854
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3854
 
AvaI C'yCGr_G

Cuts at:    874     874 
   Size:       3854
 
BamHI G'GATC_C

Cuts at:    879     879 
   Size:       3854
 
BanI G'GyrC_C

Cuts at:    870    1284    1532    1567     870 
   Size:        414     248      35    3157

  Fragments arranged by size: 

                3157     414     248      35
 
BanII G_rGCy'C

Cuts at:    868    1898     868 
   Size:       1030    2824
 
BbeI G_GCGC'C

Cuts at:   1288    1536    1288 
   Size:        248    3606
 
BbsI GAAGACnn'nnnn_

Cuts at:    319     642     319 
   Size:        323    3531
 
BciVI GTATCCnnnnn_n'

Cuts at:   1752    2808    3854    1752 
   Size:       1056    1046    1752

  Fragments arranged by size: 

                1752    1056    1046
 
BclI T'GATC_A

Cuts at:    431    1374     431 
   Size:        943    2911
 
BglI GCCn_nnn'nGGC

Cuts at:    145     154     145 
   Size:          9    3845
 
BglII A'GATC_T

Cuts at:   1369    3406    1369 
   Size:       2037    1817
 
BlpI GC'TnA_GC

Cuts at:    409     966     409 
   Size:        557    3297
 
Bme1580I G_kGCm'C

Cuts at:    273    1479    1572    2696     273 
   Size:       1206      93    1124    1431

  Fragments arranged by size: 

                1431    1206    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    447    1066    1147    1482    3626     447 
   Size:        619      81     335    2144     675

  Fragments arranged by size: 

                2144     675     619     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2221    2221 
   Size:       3854
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    306     306 
   Size:       3854
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1030    1909    2623    2921    1030 
   Size:        879     714     298    1963

  Fragments arranged by size: 

                1963     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    442    3558     442 
   Size:       3116     738
 
BsaAI yAC'GTr

Cuts at:   1207    1207 
   Size:       3854
 
BsaBI GATnn'nnATC

Cuts at:      6    1368    3405       6 
   Size:       1362    2037     455

  Fragments arranged by size: 

                2037    1362     455
 
BsaHI Gr'CG_yC

Cuts at:    716    1285    1533    2235    3731     716 
   Size:        569     248     702    1496     839

  Fragments arranged by size: 

                1496     839     702     569     248
 
BsaWI w'CCGG_w

Cuts at:    523    1043    1564    2242    2653    2800    3074     523 
   Size:        520     521     678     411     147     274    1303

  Fragments arranged by size: 

                1303     678     521     520     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    379     409     733     745     763     775     379 
   Size:         30     324      12      18      12    3458

  Fragments arranged by size: 

                3458     324      30      18      12      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    772     772 
   Size:       3854
 
BseYI C'CCAG_C

Cuts at:   1279    2037    2702    3110    1279 
   Size:        758     665     408    2023

  Fragments arranged by size: 

                2023     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    151     151 
   Size:       3854
 
BsiEI CG_ry'CG

Cuts at:    104    1396    1442    2672     104 
   Size:       1292      46    1230    1286

  Fragments arranged by size: 

                1292    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    599     868    1646    1836    2696     599 
   Size:        269     778     190     860    1757

  Fragments arranged by size: 

                1757     860     778     269     190
 
Bsp1286I G_dGCh'C

Cuts at:    273     599     868    1479    1572    1646    1836    1898    2696 
   Size:        326     269     611      93      74     190      62     798

Cuts at:   2696     273 
   Size:       1431

  Fragments arranged by size: 

                1431     798     611     326     269     190      93      74
                  62
 
BspHI T'CATG_A

Cuts at:   2286    2286 
   Size:       3854
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    904    1118    1420    1801     904 
   Size:        214     302     381    2957

  Fragments arranged by size: 

                2957     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     278    2146    2200    3684    3849      50 
   Size:        228    1868      54    1484     165      55

  Fragments arranged by size: 

                1868    1484     228     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    336    1766     336 
   Size:       1430    2424
 
BsrFI r'CCGG_y

Cuts at:   1852    2033    1852 
   Size:        181    3673
 
BssSI C'ACGA_G

Cuts at:    580    2125    2833     580 
   Size:       1545     708    1601

  Fragments arranged by size: 

                1601    1545     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    129    1231     129 
   Size:       1102    2752
 
BstBI TT'CG_AA

Cuts at:   2215    2215 
   Size:       3854
 
BstYI r'GATC_y

Cuts at:      1     662     879    1346    1369    1704    1950    2256    2268 
   Size:        661     217     467      23     335     246     306      12

Cuts at:   2268    2354    3406       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     661     467     449     335     306     246     217
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     179     107 
   Size:         72    3782
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    705    1804    1985    2148     705 
   Size:       1099     181     163    2411

  Fragments arranged by size: 

                2411    1099     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1097    1097 
   Size:       3854
 
DraI TTT'AAA

Cuts at:    853     853 
   Size:       3854
 
DrdI GACnn_nn'nnGTC

Cuts at:   1560    2904    1560 
   Size:       1344    2510
 
EaeI y'GGCC_r

Cuts at:    146     176     182     518     695    1439    1613    2004    2031 
   Size:         30       6     336     177     744     174     391      27

Cuts at:   2031    3376     146 
   Size:       1345     624

  Fragments arranged by size: 

                1345     744     624     391     336     177     174      30
                  27       6
 
EagI C'GGCC_G

Cuts at:   1439    1439 
   Size:       3854
 
EarI CTCTTCn'nnn_

Cuts at:    285    1877    2087     285 
   Size:       1592     210    2052

  Fragments arranged by size: 

                2052    1592     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    172    2062    2804    2950    3664     172 
   Size:       1890     742     146     714     362

  Fragments arranged by size: 

                1890     742     714     362     146
 
EcoICRI GAG'CTC

Cuts at:    866     866 
   Size:       3854
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    306     499     533    1679    2111    2464    3126     306 
   Size:        193      34    1146     432     353     662    1034

  Fragments arranged by size: 

                1146    1034     662     432     353     193      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:    742     742 
   Size:       3854
 
EcoO109I rG'GnC_Cy

Cuts at:    993     993 
   Size:       3854
 
EcoRI G'AATT_C

Cuts at:    858     858 
   Size:       3854
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1873    1873 
   Size:       3854
 
FspI TGC'GCA

Cuts at:   1635    1635 
   Size:       3854
 
HaeII r_GCGC'y

Cuts at:    165    1288    1536    2766    3537     165 
   Size:       1123     248    1230     771     482

  Fragments arranged by size: 

                1230    1123     771     482     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    202     234    1696    1726    1728    1758     202 
   Size:         32    1462      30       2      30    2298

  Fragments arranged by size: 

                2298    1462      32      30      30       2
 
HincII GTy'rAC

Cuts at:    893     893 
   Size:       3854
 
KasI G'GCGC_C

Cuts at:   1284    1532    1284 
   Size:        248    3606
 
KpnI G_GTAC'C

Cuts at:    874     874 
   Size:       3854
 
MluI A'CGCG_T

Cuts at:   3287    3287 
   Size:       3854
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    365    2613    2797     365 
   Size:       2248     184    1422

  Fragments arranged by size: 

                2248    1422     184
 
MscI TGG'CCA

Cuts at:    520     697    1615     520 
   Size:        177     918    2759

  Fragments arranged by size: 

                2759     918     177
 
MslI CAynn'nnrTG

Cuts at:   1843    1970    1843 
   Size:        127    3727
 
MspA1I CmG'CkG

Cuts at:    181     965    1279    1639    2423    2668     181 
   Size:        784     314     360     784     245    1367

  Fragments arranged by size: 

                1367     784     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   2035    2035 
   Size:       3854
 
NarI GG'CG_CC

Cuts at:   1285    1533    1285 
   Size:        248    3606
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3854
 
NgoMIV G'CCGG_C

Cuts at:   2033    2033 
   Size:       3854
 
NspI r_CATG'y

Cuts at:    603     907    1407    3010     603 
   Size:        304     500    1603    1447

  Fragments arranged by size: 

                1603    1447     500     304
 
PciI A'CATG_T

Cuts at:   3006    3006 
   Size:       3854
 
PfoI T'CCnGG_A

Cuts at:    283     283 
   Size:       3854
 
PmeI GTTT'AAAC

Cuts at:    853     853 
   Size:       3854
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2300    2332    2300 
   Size:         32    3822
 
PstI C_TGCA'G

Cuts at:    324     901    1586     324 
   Size:        577     685    2592

  Fragments arranged by size: 

                2592     685     577
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3854
 
PvuII CAG'CTG

Cuts at:   1279    1639    1279 
   Size:        360    3494
 
RsrII CG'GwC_CG

Cuts at:   2049    2049 
   Size:       3854
 
SacI G_AGCT'C

Cuts at:    868     868 
   Size:       3854
 
SacII CC_GC'GG

Cuts at:    182     182 
   Size:       3854
 
SalI G'TCGA_C

Cuts at:    891     891 
   Size:       3854
 
SapI GCTCTTCn'nnn_

Cuts at:    285    1877    2087     285 
   Size:       1592     210    2052

  Fragments arranged by size: 

                2052    1592     210
 
SbfI CC_TGCA'GG

Cuts at:    901     901 
   Size:       3854
 
ScaI AGT'ACT

Cuts at:   3371    3371 
   Size:       3854
 
SfcI C'TryA_G

Cuts at:     33     320     567     897    1582    2550    2741      33 
   Size:        287     247     330     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     330     287     247     191
 
SfoI GGC'GCC

Cuts at:   1286    1534    1286 
   Size:        248    3606
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3854
 
SmaI CCC'GGG

Cuts at:    876     876 
   Size:       3854
 
SmlI C'TyrA_G

Cuts at:   1009    1924    2638    2900    1009 
   Size:        915     714     262    1963

  Fragments arranged by size: 

                1963     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3854
 
SphI G_CATG'C

Cuts at:    907    1407     907 
   Size:        500    3354
 
StuI AGG'CCT

Cuts at:    565     629     565 
   Size:         64    3790
 
StyI C'CwwG_G

Cuts at:    107     988    1342     107 
   Size:        881     354    2619

  Fragments arranged by size: 

                2619     881     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    625    1437    1570    2029    2238     625 
   Size:        812     133     459     209    2241

  Fragments arranged by size: 

                2241     812     459     209     133
 
TatI w'GTAC_w

Cuts at:   3369    3369 
   Size:       3854
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    379     411    3371    3403     379 
   Size:         32    2960      32     830

  Fragments arranged by size: 

                2960     830      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1651    1651 
   Size:       3854
 
XbaI T'CTAG_A

Cuts at:    885     885 
   Size:       3854
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1349    1349 
   Size:       3854
 
XmaI C'CCGG_G

Cuts at:    874     874 
   Size:       3854
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI   Bpu10I    BsiWI     BsmI    BsmBI    BspEI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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