Restriction map for pF1KB4712
 (Linear) (Six-base) MAPSORT of: KIBB4712.seq  Check: 8345  from: 1  to: 741



KIBB4712.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AclI AA'CG_TT

Cuts at:      0     152     741 
   Size:        152     589
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     425     741 
   Size:        391      34     316

  Fragments arranged by size: 

                 391     316      34
 
AfeI AGC'GCT

Cuts at:      0      55     741 
   Size:         55     686
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     534     741 
   Size:        211     323     207

  Fragments arranged by size: 

                 323     211     207
 
BclI T'GATC_A

Cuts at:      0     323     741 
   Size:        323     418
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37      46     741 
   Size:         37       9     695

  Fragments arranged by size: 

                 695      37       9
 
BlpI GC'TnA_GC

Cuts at:      0     301     741 
   Size:        301     440
 
Bme1580I G_kGCm'C

Cuts at:      0     165     741 
   Size:        165     576
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     339     741 
   Size:        339     402
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     741 
   Size:        198     543
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     334     741 
   Size:        334     407
 
BsaHI Gr'CG_yC

Cuts at:      0     608     741 
   Size:        608     133
 
BsaWI w'CCGG_w

Cuts at:      0     415     741 
   Size:        415     326
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     271     301     625     637     655     667     741 
   Size:        271      30     324      12      18      12      74

  Fragments arranged by size: 

                 324     271      74      30      18      12      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     664     741 
   Size:        664      77
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     741 
   Size:         43     698
 
BsiHKAI G_wGCw'C

Cuts at:      0     491     741 
   Size:        491     250
 
Bsp1286I G_dGCh'C

Cuts at:      0     165     491     741 
   Size:        165     326     250

  Fragments arranged by size: 

                 326     250     165
 
BsrBI CCG'CTC

Cuts at:      0     170     741 
   Size:        170     571
 
BsrDI GCAATG_nn'

Cuts at:      0     228     741 
   Size:        228     513
 
BssSI C'ACGA_G

Cuts at:      0     472     741 
   Size:        472     269
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      21     741 
   Size:         21     720
 
BstYI r'GATC_y

Cuts at:      0     554     741 
   Size:        554     187
 
BtgI C'CryG_G

Cuts at:      0      71     741 
   Size:         71     670
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     597     741 
   Size:        597     144
 
EaeI y'GGCC_r

Cuts at:      0      38      68      74     410     587     741 
   Size:         38      30       6     336     177     154

  Fragments arranged by size: 

                 336     177     154      38      30       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     177     741 
   Size:        177     564
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      64     741 
   Size:         64     677
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     391     425     741 
   Size:        198     193      34     316

  Fragments arranged by size: 

                 316     198     193      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     634     741 
   Size:        634     107
 
HaeII r_GCGC'y

Cuts at:      0      57     741 
   Size:         57     684
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      94     126     741 
   Size:         94      32     615

  Fragments arranged by size: 

                 615      94      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     741 
   Size:        257     484
 
MscI TGG'CCA

Cuts at:      0     412     589     741 
   Size:        412     177     152

  Fragments arranged by size: 

                 412     177     152
 
MspA1I CmG'CkG

Cuts at:      0      73     741 
   Size:         73     668
 
NspI r_CATG'y

Cuts at:      0     495     741 
   Size:        495     246
 
PfoI T'CCnGG_A

Cuts at:      0     175     741 
   Size:        175     566
 
PstI C_TGCA'G

Cuts at:      0     216     741 
   Size:        216     525
 
SacII CC_GC'GG

Cuts at:      0      74     741 
   Size:         74     667
 
SapI GCTCTTCn'nnn_

Cuts at:      0     177     741 
   Size:        177     564
 
SfcI C'TryA_G

Cuts at:      0     212     459     741 
   Size:        212     247     282

  Fragments arranged by size: 

                 282     247     212
 
StuI AGG'CCT

Cuts at:      0     457     521     741 
   Size:        457      64     220

  Fragments arranged by size: 

                 457     220      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     517     741 
   Size:        517     224
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     271     303     741 
   Size:        271      32     438

  Fragments arranged by size: 

                 438     271      32
 

 Enzymes that do cut:

     AclI     AcuI     AfeI     BbsI     BclI     BglI     BlpI Bme1580I
     BmrI     BpmI     BsaI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
  BsiHKAI Bsp1286I    BsrBI    BsrDI    BssSI   BstAPI    BstYI     BtgI
    BtgZI     EaeI     EarI     EciI  Eco57MI    EcoNI    HaeII    Hin4I
     MmeI     MscI   MspA1I     NspI     PfoI     PstI    SacII     SapI
     SfcI     StuI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI    BglII    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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