Restriction map for pF1KB4700
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AhdI        
Alw44I      ApaI        ApaLI       AscI        AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BclI        BfaI        BfrI        BglI        
BlnI        BsaAI       BseAI       BsiWI       BsmBI       
BsmI        BspEI       BspHI       BspMI       BsrBI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstXI       DraIII      Eam1105I    EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HpaI        
KasI        KspI        MaeI        MluI        MroI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NsiI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SmaI        
SnaBI       SpeI        Sse8387I    StuI        SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

Acc65I       1537  GGTACC               G^GTACC
AcyI           60  GGCGTC               G[GA]^CG[CT]C
AgeI         2164  ACCGGT               A^CCGGT
AocI          829  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        18  AGCGCT               AGC^GCT
Asp700I      1522  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1537  GGTACC               G^GTACC
AspHI        2610  GAGCTC               G[AT]GC[AT]^C
AvaI          645  CTCGGG               C^[CT]CG[GA]G
BalI          453  TGGCCA               TGG^CCA
BbsI         2341  GAAGAC               GAAGAC(2/6)
BciVI        2457  GTATCC               GTATCC(6/5)
BglII        1775  AGATCT               A^GATCT
BlpI         1984  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1984  GCTCAGC              GC^T[ACGT]AGC
BpuAI        2341  GAAGAC               GAAGAC(2/6)
BsaBI        1219  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          60  GGCGTC               G[GA]^CG[CT]C
BsaI          328  GAGACC               GGTCTC(1/5)
BsiEI        1100  CGGCCG               CG[GA][CT]^CG
BsiHKAI      2610  GAGCTC               G[AT]GC[AT]^C
BsoBI         645  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1256  TGTACA               T^GTACA
BspDI        1574  ATCGAT               AT^CGAT
BsrGI        1256  TGTACA               T^GTACA
Bst1107I     1032  GTATAC               GTA^TAC
BstAPI        871  GCAATTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      1032  GTATAC               GTA^TAC
Bsu36I        829  CCTGAGG              CC^T[ACGT]AGG
CelII        1984  GCTCAGC              GC^T[ACGT]AGC
ClaI         1574  ATCGAT               AT^CGAT
CpoI         2462  CGGTCCG              CG^G[AT]CCG
DrdI         1321  GACGGGTTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1100  CGGCCG               C^GGCCG
EclXI        1100  CGGCCG               C^GGCCG
Eco47III       18  AGCGCT               AGC^GCT
Eco52I       1100  CGGCCG               C^GGCCG
Eco81I        829  CCTGAGG              CC^T[ACGT]AGG
EcoNI          51  CCTGCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1412  CCAAGG               C^C[AT][AT]GG
HaeII          18  AGCGCT               [GA]GCGC^[CT]
HgaI           61  GCGTC                GACGC(5/10)
Hin1I          60  GGCGTC               G[GA]^CG[CT]C
HindIII       273  AAGCTT               A^AGCTT
KpnI         1537  GGTACC               GGTAC^C
MamI         1219  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         453  TGGCCA               TGG^CCA
MscI          453  TGGCCA               TGG^CCA
NruI          678  TCGCGA               TCG^CGA
PinAI        2164  ACCGGT               A^CCGGT
PshAI        1116  GACAGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        2462  CGGTCCG              CG^G[AT]CCG
SacI         2610  GAGCTC               GAGCT^C
SexAI         806  ACCAGGT              A^CC[AT]GGT
SgrAI        2163  CACCGGTG             C[GA]^CCGG[CT]G
SphI          294  GCATGC               GCATG^C
SspBI        1256  TGTACA               T^GTACA
StyI         1412  CCAAGG               C^C[AT][AT]GG
XmnI         1522  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1032  GTATAC               GT^[AC][GT]AC
             1186  GTAGAC              
AccII         679  CGCG                 CG^CG
             2089  CGCG                
AflIII        956  ACATGT               A^C[GA][CT]GT
             2696  ACATGT              
AlwNI         599  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              824  CAGTTCCTG           
AspI          478  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              754  GACATTGTC           
BanII        2449  GAGCCC               G[GA]GC[CT]^C
             2610  GAGCTC              
BcnI          692  CCCGG                CC^[GC]GG
             2244  CCGGG               
BsaWI        2164  ACCGGT               [AT]^CCGG[AT]
             2460  TCCGGT              
BseRI         385  GAGGAG               GAGGAG(10/8)
             2333  GAGGAG              
BsgI         2124  CTGCAC               GTGCAG(16/14)
              125  GTGCAG              
BspLU11I      956  ACATGT               A^CATGT
             2696  ACATGT              
BsrDI        2175  CATTGC               GCAATG(2/0)
              188  GCAATG              
BsrFI        1534  GCCGGT               [GA]^CCGG[CT]
             2164  ACCGGT              
BstUI         679  CGCG                 CG^CG
             2089  CGCG                
CfoI           19  GCGC                 GCG^C
             2088  GCGC                
Cfr10I       1534  GCCGGT               [GA]^CCGG[CT]
             2164  ACCGGT              
DraI         1995  TTTAAA               TTT^AAA
             2310  TTTAAA              
EaeI          453  TGGCCA               [CT]^GGCC[GA]
             1100  CGGCCG              
EcoRI        1418  GAATTC               G^AATTC
             1742  GAATTC              
HhaI           19  GCGC                 GCG^C
             2088  GCGC                
HinP1I         19  GCGC                 G^CGC
             2088  GCGC                
HincII       1437  GTCAAC               GT[CT]^[GA]AC
             1545  GTTGAC              
HindII       1437  GTCAAC               GT[CT]^[GA]AC
             1545  GTTGAC              
MvnI          679  CGCG                 CG^CG
             2089  CGCG                
NciI          692  CCCGG                CC^[GC]GG
             2244  CCGGG               
PflFI         478  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              754  GACATTGTC           
PflMI        1894  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2479  CCACACCCTGG         
Tth111I       478  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              754  GACATTGTC           
Van91I       1894  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2479  CCACACCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        AvaII       BanI        
BbvI        BcgI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MfeI        MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MwoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PshBI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       
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